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| Registros recuperados : 8 | |
| 1. |  | KRUG, M. G. Z.; STIPP, L. C. L.; RODRIGUEZ, A. P. M.; MENDES, B. M. J. In vitro organogenesis in watermelon cotyledons. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 9, p. 861-865, set. 2005 Título em português: Organogênese in vitro em cotilédones de melancia.| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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| 4. | | BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | ATTÍLIO, L. B.; MOURÃO FILHO, F. de A. A.; HARAKAVA, R.; SILVA, T. L. da; MIYATA, L. Y.; STIPP, L. C. L.; MENDES, B. M. J. Genetic transformation of sweet oranges with the D4E1 gene driven by tha AtPP2 promoter. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 7, p. 741-747, jul. 2013. Título em português: Transformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | ASTUA, J. de F.; DOCEMA, M. L.; STIPP, L. C. L.; HARAKAVA, R.; TAVANO, E. C. R.; ATTILIO, T. S. M. Transgenic "Hamlin" sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to huanglongbing and greater callose accumulation in the leaves. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Os avanços da Fitopatologia na Era Genômica: anais. Recife: SBF: UFPRE/PPGF, 2019. 1 p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | MENDONÇA, JÉSSIKA-ANGELOTTI; OLIVEIRA, P. N. de; ANSANTE, N. F.; STIPP, L. C. L.; ASTUA, J. de F.; HURTADO, F. M. M.; BELASQUE, J.; MOURÃO FILHO, F. de A. A. Expression of the Citrus sinensis EDS5 gene, MATE family, in Solanum lycopersicum L. cv. Micro?Tom enhances resistance to tomato spot disease. Tropical Plant Pathology, January, 2022.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | DOCEMA, M. L.; MORAES, T. DE S.; ATTÍLIO, L. B.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; BELASQUE JUNIOR, J.; AZEVEDO, R. A.; GAZIOLA, S. A.; MOURÃO FILHO, F. DE A. A. Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease. Plant Cell, Tissue and Organ Culture, 2022.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 8 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. |
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Título: |
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. |
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ISSN: |
1806-9967 |
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Idioma: |
Português |
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Conteúdo: |
New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
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Marc: |
LEADER 02150naa a2200205 a 4500
001 2136203
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
022 $a1806-9967
100 1 $aBEZERRA, Y. C. dos A.
245 $aHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.$h[electronic resource]
260 $c2021
520 $aNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
650 $aFruta Cítrica
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aATTÍLIO, L. B.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 43, n. 4,e-005, 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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