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| Registros recuperados : 366 | |
| 122. |  | SOUZA, F. V. D.; GARCIA-SOGO, B.; SOUZA, A. da S.; SAN-JUÁN, A. P.; MORENO, V. Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro. Brazilian Archives of Biology and Technology, Curitiba, v. 49, n. 1, p.21-27, Jan. 2006.| Biblioteca(s): Embrapa Hortaliças. |
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| 123. | | MENEZES, M. C.; VIDAL, A. M.; SANTA ROSA, S.; SOUZA, K. A. de; SOUZA, A. da S. Suplementação do meio de cultivo para a micropropagação da mandioca. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 124. | | CARMO JUNIOR, U. R.; SOARES FILHO, W. dos S.; SOUZA, A. da S.; GESTEIRA, A. da S. Tangerineiras triploides de citros com frutos sem sementes. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro : [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 127. | | SILVEIRA, D. G.; SOUZA, A. da S.; VIDAL, Á. M.; OLIVEIRA, E. J. de; MENEZES, M. C. Embriogênese somática no mamoeiro 'Tainung nº 1' influenciada pelos cotilédones e doses de 2,4-D. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 128. | | SILVEIRA, D. G.; MORAIS-LINO, L. S.; SOUZA, F. V. D.; SOUZA, A. da S.; SANTANA, J. R. F. de. Embriogêneses somática em caroá. Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, p.1230-1234, 2007. Suplemento. Edição dos Resumos do XVI Congresso Brasileiro de Floricultura e Plantas Ornamentais; III Congresso Brasileiro de Cultura de Tecidos e Plantas; I Simpósio de Plantas Ornamentais Nativas, Goiânia, set. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 129. | | JUNGHANS, T. G.; SIMÕES, K. da S.; CALDAS, C. dos S.; SOUZA, A. da S.; ANDRADE, S. R. M. de. Estabelecimento in vitro de ápices caulinares de Passiflora edulis Sims visando limpeza clonal. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 130. | | JUNGHANS, T. G.; SIMÕES, K. da S.; CALDAS, C. dos S.; SOUZA, A. da S.; ANDRADE, S. R. M. de. Estabelecimento in vitro de ápices caulinares de Passiflora edulis Sims visando limpeza clonal. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 133. | | JUNGHANS, T. G.; CALDAS, C. dos S.; SIMÕES, K. da S.; SOUZA, A. da S.; ANDRADE, S. R. M. de. Estabelecimento in vitro de ápices caulinares provenientes de plantas adultas de Passiflora alata Curtis visando limpeza clonal. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Biblioteca(s): Embrapa Cerrados; Embrapa Mandioca e Fruticultura. |
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| 135. | | JUNGHANS, T. G.; SIMÕES, K. da S.; CALDAS, C. dos S.; SOUZA, A. da S.; ANDRADE, S. R. M. de. Comportamento in vitro de ápices caulinares provenientes de plantas adultas de Passiflora alata Curtis. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 136. | | JUNGHANS, T. G.; SIMÕES, K. da S.; CALDAS, C. dos S.; SOUZA, A. da S.; ANDRADE, S. R. M. D. Comportamento in vitro de ápices caulinares provenientes de plantas adultas de Passiflora alata Curtis In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 12.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4., 2009, Aracajú. Ciência, inovação e sustentabilidade: [resumos]. Aracajú: Embrapa Tabuleiro Costeiros; Cruz das Almas: Embrapa Mandioca e fruticultura, 2009. 1 CD-ROM. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 366 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/11/2017 |
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Data da última atualização: |
06/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, M. J. S.; OLIVEIRA, E. J. de; SOUZA, A. da S.; PEREIRA, J. S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de. |
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Afiliação: |
M. J. S. CARVALHO; EDER JORGE DE OLIVEIRA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; J. S. PEREIRA; M. S .A. S. DIAMANTINO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
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Título: |
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n.2, 2017. |
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Série: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. MenosThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic ro... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus NAL: |
cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02559naa a2200217 a 4500
001 2078909
005 2017-11-06
008 2017 bl --- 0-- u #d
100 1 $aCARVALHO, M. J. S.
245 $aCleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture.$h[electronic resource]
260 $c2017
490 $a1676-5680
520 $aThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease.
650 $acassava
650 $aMandioca
700 1 $aOLIVEIRA, E. J. de
700 1 $aSOUZA, A. da S.
700 1 $aPEREIRA, J. S.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aOLIVEIRA, S. A. S. de
773 $tGenetics and Molecular Research$gv. 16, n.2, 2017.
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