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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/08/2013 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; NOGUEIRA, E. O. P. L.; TINOCO, M. L. P.; FARIA, J. C. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, CENARGEN; ELSA OLIVEIRA P E LAGO NOGUEIRA, CENARGEN; MARIA LAINE P. TINOCO, CENARGEN; JOSIAS CORREA DE FARIA, CNPAF. |
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Título: |
Molecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Biotechnology, Amsterdam, v. 66, n. 1/2, p. 42-50, June 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.jbiotec.2013.04.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Golden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market. MenosGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the ... Mostrar Tudo |
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Thesagro: |
Engenharia genética; Feijão. |
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Thesaurus Nal: |
Begomovirus; Dry beans; Geminiviridae; Genetic engineering; RNA interference. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02424naa a2200253 a 4500
001 1968340
005 2023-03-08
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jbiotec.2013.04.009$2DOI
100 1 $aARAGÃO, F. J. L.
245 $aMolecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus.$h[electronic resource]
260 $c2013
520 $aGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market.
650 $aBegomovirus
650 $aDry beans
650 $aGeminiviridae
650 $aGenetic engineering
650 $aRNA interference
650 $aEngenharia genética
650 $aFeijão
700 1 $aNOGUEIRA, E. O. P. L.
700 1 $aTINOCO, M. L. P.
700 1 $aFARIA, J. C.
773 $tJournal of Biotechnology, Amsterdam$gv. 66, n. 1/2, p. 42-50, June 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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| Registros recuperados : 40 | |
| 1. |  | RAGA, A.; SOUZA FILHO, M. F.; THOMAZINI, M. J.; RAMIRO, Z. A. Ação de tubos mata-moscas sobre adultos de Ceratitis capitata, em condições de laboratório. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 17.; ENCONTRO NACIONAL DE FITOSSANITARISTAS, 8., 1998, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: Universidade Federal Rural do Rio de Janeiro, 1998. v. 1, p. 472.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 2. | | SAVARIS, M.; LAMPERT, S.; MARSARO JÚNIOR, A. L.; ADAIME, R.; SOUZA FILHO, M. F. de. First record of Anastrepha fraterculus and Ceratitis capitata (Diptera, Tephritidae) on Arecaceae in Brazil. The Florida Entomologist, Gainesville, v. 96, n. 4, p. 1597-1599, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amapá; Embrapa Trigo. |
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| 3. | | ADAIME, R.; STRIKIS, P. C.; SOUZA-FILHO, M. F. de; LIMA, C. R.; LASA, R. First report of Lonchaeidae (Diptera) infesting fruits of Byrsonima crassifolia in Brazil. Revista Colombiana de Entomología, Bogotá, v. 38, n. 2, p. 363-364, 2012.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Amapá. |
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| 4. | | MARSARO JUNIOR, A. L.; SOUZA-FILHO, M. F. de; SILVA, R. A. da; STRIKIS, P. C. First report of natural infestation of Pereskia aculeata Mill., (Cactaceae) by Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) in Brazil. Revista de Agricultura, Piracicaba, v. 86, n. 2, p. 151-154, set. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Amapá. |
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| 6. | | DEUS, E. G.; ADAIME, R.; JESUS, C. R.; SOUZA-FILHO, M. F. Primeiro registro de Anastrepha shannoni Stone (Diptera: Tephritidae) no Estado do Amapá, Brasil. Arquivos do Instituto Biológico, São Paulo, v. 76, n. 4, p. 725-728, out./dez. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amapá. |
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| 8. | | SILVA, R. A. da; SOUZA, M. S. M.; SILVA, W. R.; SOUZA FILHO, M. F. Novo registro de hospedeiro de Anastrepha coronilli Carrejo & González (Diptera: Tephritidae) no Brasil. O Biológico, São Paulo, v. 71, n. 2, p. 135, 2009. Edição dos Resumos da 22ª Reunião Anual do Instituto Biológico- RAIB, São Paulo, nov. 2009. Resumo 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 10. | | SOUZA FILHO, M. F. de; SUPLICY FILHO, N; SATO, M. E.; TAKEMATSU, A. P. Suscetibilidade do ácaro-rajado proviniente de videira de Pilar do Sul, SP, a diversos acaricidas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 29, n. 8, p. 1187-1192. ago. 1994. Título em inglês: Susceptibility of the two-spotted spider mite collected from grape crop (Vitis spp.) in Pilar do Sul, São Paulo, Brazil, to some acaricides.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | MARSARO JÚNIOR, A. L.; ADAIME, R.; SOUZA FILHO, M. F. de; LIMA, C. R; TRASSATO, L. C. Anastrepha fruit flies (Diptera, Tephritidae) from two municipalites of the state of Roraima, Brazil, with three new records. Revista de Agricultura, Piracicaba, v. 88, n. 1, p. 41-43, jun. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amapá; Embrapa Trigo. |
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| 13. | | JESUS, C. R. de; OLIVEIRA, M. N. de; SOUZA FILHO, M. F. de; SILVA, R. A. da; ZUCCHI, R. A. First record of Anastrepha parishi Stone (Diptera, Tephritidae) and its host in Brazil. Revista Brasileira de Entomologia, v. 52, n. 1, p. 135-136, mar. 2008. Título em português: Primeiro registro de Anastrepha parishi Stone (Diptera, Tephritidae) e seu hospedeiro no Brasil.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Amapá. |
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| 14. | | ADAIME, R.; MARSARO JUNIOR, A. L.; SOUZA-FILHO, M. F.; CHAGAS, E. A.; LIMA, C. G. B. New host of Anastrepha parishi Stone (Diptera: Tephritidae) reported in Brazil. Brazilian Journal of Biology, São Carlos, v. 72, n. 1, p. 227, Feb. 2012.| Tipo: Nota Técnica/Nota Científica | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amapá; Embrapa Trigo. |
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| 15. | | SILVA, R. A. da; MARSARO JUNIOR, A. L.; SOUZA-FILHO, M. F.; CHAGAS, E. A.; LIMA, C. G. B. New host of Anastrepha Parishi Stone (Diptera: Tephritidae) reported in Brazil. Brazilian Journal of Biology, São Carlos, v. 72, n. 1, p. 227, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Roraima. |
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| 18. | | BARROS, C. R. de J.; ADAIME, R.; OLIVEIRA, M. N.; SILVA, W. R.; COSTA NETO, S. V.; SOUZA FILHO, M. F. Anastrepha (Diptera: Tephritidae) species, their hosts and parasitoids (Hymenoptera: Braconidae) in five municipalities of the State of Amapá, Brazil. Florida Entomologist, Florida, v. 95, n. 3, p. 694-705, Sep. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amapá. |
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| 19. | | SILVA, R. A. da; DEUS, E. da G. de; PEREIRA, J. D. B.; JESUS, C. R. de; SOUZA-FILHO, M. F. de; ZUCCHI, R. A. Conhecimento sobre moscas-das-frutas no Estado do Amapá. In: SILVA, R. A. da; LEMOS, W. de P.; ZUCCHI, R. A. (Ed.). Moscas-das-frutas na Amazônia brasileira: diversidade, hospedeiros e inimigos naturais. Macapá: Embrapa Amapá, 2011. p. 223-236.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amapá. |
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| 20. | | ARAÚJO, A. S.; ZUCCHI, R. A.; NORRBOM, A. L.; NANINI, F.; CORRÊA, A. S.; ALVARENGA, C. D.; SOUZA-FILHO, M. F.; NAVA, D. E.; SAVARIS, M. Integrative approach reveals the identity of Brazilian specimens previously recognized as Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae). Zootaxa, v. 5228, n. 3, p. 317–336, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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