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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/01/2009 |
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Data da última atualização: |
18/01/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
MARTINS, M. L.; FELIPE, C. A. S.; OLIVEIRA, M. G. de C.; BASSINELLO, P. Z.; SIMÕES, D. M.; VITORINO, G. F. |
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Afiliação: |
Maria Lúcia Martins, bolsista CNPq; Carlos Alberto Severo Felipe, UEG Ipameri; Márcia Gonzaga de Castro Oliveira, mestranda UEG Anápolis; PRISCILA ZACZUK BASSINELLO, CNPAF; Douglas Meyer Simões, UEG Ipameri; Gabriel Freitas Vitorino, UEG Ipameri. |
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Título: |
Comparação entre diferentes formas de fornecer micronutrientes às sementes de feijão. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. |
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Descrição Física: |
1 CD-ROM. |
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Série: |
(IAC. Documentos, 85). |
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Idioma: |
Português |
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Conteúdo: |
O presente trabalho teve como objetivo comparar diferentes formas de aplicação dos micronutrientes: Boro (B), Cobalto (Co) e Molibdênio (Mo) em sementes de feijão. As formas de aplicação avaliadas foram: recobrimento de sementes em leito de jorro, recobrimento em sacos plásticos e o fornecimento via sulco. |
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Palavras-Chave: |
CONAFE; Micronutriente. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Semente. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74760/1/155.pdf
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Marc: |
LEADER 01135nam a2200253 a 4500
001 1217672
005 2013-01-18
008 2008 bl uuuu u00u1 u #d
100 1 $aMARTINS, M. L.
245 $aComparação entre diferentes formas de fornecer micronutrientes às sementes de feijão.
260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico$c2008
300 $c1 CD-ROM.
490 $a(IAC. Documentos, 85).
520 $aO presente trabalho teve como objetivo comparar diferentes formas de aplicação dos micronutrientes: Boro (B), Cobalto (Co) e Molibdênio (Mo) em sementes de feijão. As formas de aplicação avaliadas foram: recobrimento de sementes em leito de jorro, recobrimento em sacos plásticos e o fornecimento via sulco.
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aSemente
653 $aCONAFE
653 $aMicronutriente
700 1 $aFELIPE, C. A. S.
700 1 $aOLIVEIRA, M. G. de C.
700 1 $aBASSINELLO, P. Z.
700 1 $aSIMÕES, D. M.
700 1 $aVITORINO, G. F.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
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Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
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Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Santa ines. |
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Thesagro: |
Ovis Aries. |
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Thesaurus NAL: |
cryopreservation; gene expression. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02970naa a2200289 a 4500
001 2085659
005 2023-01-27
008 2018 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource]
260 $c2018
520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $acryopreservation
650 $agene expression
650 $aOvis Aries
653 $aIn vivo embryo production
653 $aSanta ines
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDAO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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