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| Registros recuperados : 142 | |
| 6. |  | FONSECA, J. F. da; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SILVA, M. R.; OLIVEIRA, M. E. F.; BARTLEWSKI, P. M.; SOUZA-FABJAN, J. M. G. Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin. Animal Reproduction Science, v. 203, p. 25-32, Apr. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 7. | | LEITE, C. R.; FONSECA, J. F. da; FERNANDES, D. A. M.; SOUZA-FABJAN, J. M. G.; ASCOLI, F. O.; BRANDÃO, F. Z. Cervical relaxation for non-surgical uterus access in Santa Inês ewes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.70, n. 6, p. 1671-1679, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | MORAIS, M. C. C.; ARRAIS, A. M.; SILVA, N. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Association of controlled light program, cloprostenol and gonadotropins for synchronous estrus induction and fixed time artificial insemination in dairy goats during the non-breeding season: preliminary results. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | PINTO, P. H. N.; FREITAS, J. A.; FONSECA, J. D.; PILE, E. A.; ESTEVES, L. V.; SOUZA, V. L.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Artificial insemination in Cape Verdean Goats with cooled semen stored for 24 or 48 Hours. Australian Journal of Basic and Applied Sciences, v. 8, n. 18, p. 473-478, Dec. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | FONSECA, J. F. da; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; LEITE, C. R.; NASCIMENTO-PENIDO, P. M. P.; BRANDÃO, F. Z.; LEHLOENYA, K. C. Nonsurgical embryo recovery and transfer in sheep and goats. Theriogenology, New York, v. 86, n. 1, p. 144-151, Jul. 2016.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | FONSECA, J. F. da; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BATISTA, R. I. T. P.; GARCIA, A. R.; BARTLEWSKIE, P. M.; SOUZA-FABJAN, J. M. G. Non-surgical embryo transfer in goats and sheep: the Brazilian experience. Reproduction Fertility and Development, v. 31, n. 1 p. 17-26, Dec. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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| 12. | | PINHEIRO, J. B. S.; SOUZA-FABJAN, J. M. G.; CORREIA, L. F. L.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Differences between 5- and 6-day progestogen-based oestrus induction protocol in Saanen and Toggenburg goats. Reproduction, Fertility, and Development, v. 33, n. 2, p. 166, 2021. Edição dos Proceedings of the 47th Annual Conference of the International Embryo Technology (IETS) Virtual Meeting, January 18-21, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | MAIA, A. L. R. e S.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; BRANDÃO, F. Z.; FONSECA, J. F. da. Distúrbios reprodutivos em cabras leiteiras e impactos potenciais nos sistemas de produção. Revista Acadêmica; Ciência Animal, Curitiba, v. 15, p. 77-89, 2017. Suplemento 2. Edição dos trabalhos apresentados no XI Congresso Brasileiro de Buiatria, Foz do Iguaçu, PR, 11 a 14 set. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | ROCHA, M. S.; MAIA, A. L. R. S.; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; FONSECA, J. F. da; OLIVEIRA, C. A.; SOUZA-FABJAN, J. M. G. Occurrence of premature regression of corpus luteum in MOET programs in Dorper ewes under subtropical climate. Livestock Science, v. 255, 104808, 2022.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | MAIA, A. L. R. e S.; BRANDÃO, F. Z.; VEIGA, M. de O.; SOUZA-FABJAN, J. M. G.; FACO, O.; FONSECA, J. F. da. Occurrence of hydrometra in dairy goats after estrous induction by either light program or hormonal protocol. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 499, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | SOUZA-FABJAN, J. M. G.; TORRES, C. A. A.; MAIA, A. L. R. S.; BRANDÃO, F. Z.; OBA, E.; BELTOLDO, M. J.; FONSECA, J. F. da. Re-used progesterone devices efficiently synchronise oestrus and ovulation after autoclaving process in Toggenburg goats during the breeding season. Animal Production Science, Victoria, v. 55, n. 6, p. 818-822, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; GUIMARÃES, M. P. P.; BRANDÃO, F. Z.; BARTLEWSK, P. M.; FONSECA, J. F. da. Review: Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants. Animal. The International Journal of Animal Biosciences, v. 17, e100787, May, 2023. Supplement 1. Review.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | BRAIR, V. L.; ANDRADE, A. B. P.; MORAIS, M. C. C.; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Short-term hormonal protocol efficiency either with or without equine chorionic gonadotrophin to promote oestrous synchronization in cyclic dairy goats. Reproduction, Fertility and Development, v. 33, n. 1/2, p. 165, 2021. Edição dos Proceedings of the 47th Annual Conference of the International Embryo Technology (IETS) Virtual Meeting, January 18-21, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 142 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus NAL: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
LEADER 02452naa a2200265 a 4500
001 2118709
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Embrapa Gado de Leite (CNPGL) |
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