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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Algodão; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Milho e Sorgo; Embrapa Semiárido; Embrapa Soja; Embrapa Trigo; Embrapa Uva e Vinho. |
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Data corrente: |
06/08/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARBEDO, J. G. A.; KOENIGKAN, L. V.; HALFELD-VIEIRA, B. de A.; COSTA, R. V. da; NECHET, K. de L.; GODOY, C. V.; LOBO JUNIOR, M.; PATRÍCIO, F. R. A.; TALAMINI, V.; CHITARRA, L. G.; OLIVEIRA, S. A. S. de; ISHIDA, A. K. N.; FERNANDES, J. M. C.; SANTOS, T. T.; CAVALCANTI, F. R.; TERAO, D.; ANGELOTTI, F. |
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Afiliação: |
JAYME GARCIA ARNAL BARBEDO, CNPTIA; LUCIANO VIEIRA KOENIGKAN, CNPTIA; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; RODRIGO VERAS DA COSTA, CNPMS; KATIA DE LIMA NECHET, CNPMA; CLAUDIA VIEIRA GODOY, CNPSO; MURILLO LOBO JUNIOR, CNPAF; F. R. A. PATRÍCIO, Instituto Biológico, Campinas, SP; VIVIANE TALAMINI, CPATC; LUIZ GONZAGA CHITARRA, CNPA; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; JOSE MAURICIO CUNHA FERNANDES, CNPT; THIAGO TEIXEIRA SANTOS, CNPTIA; FABIO ROSSI CAVALCANTI, CNPUV; DANIEL TERAO, CNPMA; FRANCISLENE ANGELOTTI, CPATSA. |
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Título: |
Annotated plant pathology databases for image-based detection and recognition of diseases. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
IEEE Latin America Transactions, v. 16, n. 6, p. 1749-1757, June 2018. |
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Idioma: |
Inglês
Português |
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Notas: |
Na publicação: B. A. Halfeld-Vieira, R. V. Costa, K. L. Nechet, S. A. S. Oliveira. |
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Conteúdo: |
Over the last few years, considerable effort has been spent by Embrapa in the construction of a plant disease database representative enough for the development of effective methods for automatic plant disease detection and recognition. In October of 2016, this database, called PDDB, had 2326 images of 171 diseases and other disorders affecting 21 plant species. PDDB size, although considerable, is not enough to allow the use of powerful techniques such as deep learning. In order to increase its size, each image was subdivided according to certain criteria, increasing the number of images to 46,513. Both the original (PDDB) and subdivided (XDB) databases are now being made freely available for academic research purposes, thus supporting new studies and contributing to speed up the advances in the area. Both collections are expected to grow continuously in order to expand their reach. PDDB and XDB can be accessed in the link https://www.digipathosrep.cnptia.embrapa.br/. Keywords— plant pathology, database, deep learning, image processing. |
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Palavras-Chave: |
Aprendizagem profunda; Banco de dados; Deep learning; Imagem em processamento; Patologia vegetal; Processamento de imagem. |
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Thesagro: |
Doença de Planta. |
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Thesaurus Nal: |
Databases; Image analysis; Plant diseases and disorders; Plant pathology. |
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Categoria do assunto: |
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H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182246/1/16TLA6-27GarciaArnalBarbedo.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181156/1/16TLA6-27GarciaArnalBarbedo.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198847/1/ID44389-2018v16n6p1749IEEELatinAmericaTransaction.pdf
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Marc: |
LEADER 02472naa a2200457 a 4500
001 2094883
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
100 1 $aBARBEDO, J. G. A.
245 $aAnnotated plant pathology databases for image-based detection and recognition of diseases.$h[electronic resource]
260 $c2018
500 $aNa publicação: B. A. Halfeld-Vieira, R. V. Costa, K. L. Nechet, S. A. S. Oliveira.
520 $aOver the last few years, considerable effort has been spent by Embrapa in the construction of a plant disease database representative enough for the development of effective methods for automatic plant disease detection and recognition. In October of 2016, this database, called PDDB, had 2326 images of 171 diseases and other disorders affecting 21 plant species. PDDB size, although considerable, is not enough to allow the use of powerful techniques such as deep learning. In order to increase its size, each image was subdivided according to certain criteria, increasing the number of images to 46,513. Both the original (PDDB) and subdivided (XDB) databases are now being made freely available for academic research purposes, thus supporting new studies and contributing to speed up the advances in the area. Both collections are expected to grow continuously in order to expand their reach. PDDB and XDB can be accessed in the link https://www.digipathosrep.cnptia.embrapa.br/. Keywords— plant pathology, database, deep learning, image processing.
650 $aDatabases
650 $aImage analysis
650 $aPlant diseases and disorders
650 $aPlant pathology
650 $aDoença de Planta
653 $aAprendizagem profunda
653 $aBanco de dados
653 $aDeep learning
653 $aImagem em processamento
653 $aPatologia vegetal
653 $aProcessamento de imagem
700 1 $aKOENIGKAN, L. V.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aCOSTA, R. V. da
700 1 $aNECHET, K. de L.
700 1 $aGODOY, C. V.
700 1 $aLOBO JUNIOR, M.
700 1 $aPATRÍCIO, F. R. A.
700 1 $aTALAMINI, V.
700 1 $aCHITARRA, L. G.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aISHIDA, A. K. N.
700 1 $aFERNANDES, J. M. C.
700 1 $aSANTOS, T. T.
700 1 $aCAVALCANTI, F. R.
700 1 $aTERAO, D.
700 1 $aANGELOTTI, F.
773 $tIEEE Latin America Transactions$gv. 16, n. 6, p. 1749-1757, June 2018.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/05/2017 |
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Data da última atualização: |
29/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. |
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Afiliação: |
Carolina Cerqueira Sarmento Olivares; Joanna Maria Gonçalves de Souza-Fabjan; JEFERSON FERREIRA DA FONSECA, CNPC; Mario Felipe Alvarez Balaro; Vicente José de Figueirêdo Freitas; Rodrigo Vasconcelos de Oliveira; Felipe Zandonadi Brandão. |
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Título: |
Comparison of different sperm selection techniques in ram frozen-thawed sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 45, p. 1431, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique. MenosResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of inte... Mostrar Tudo |
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Palavras-Chave: |
CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques; Sperm preparation. |
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Thesagro: |
Espermatozóide; Ovino; Reprodução animal; Sêmen. |
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Thesaurus NAL: |
Reproduction; Sheep; sperm capacitation; Spermatozoa; Treaties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160322/1/CNPC-2017-Comparison.pdf
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Marc: |
LEADER 04855naa a2200373 a 4500
001 2070178
005 2017-05-29
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different sperm selection techniques in ram frozen-thawed sperm.
260 $c2017
520 $aResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique.
650 $aReproduction
650 $aSheep
650 $asperm capacitation
650 $aSpermatozoa
650 $aTreaties
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSêmen
653 $aCASA
653 $aCTC
653 $aPlasma membrane integrity
653 $aProtocolo
653 $aReproductive techniques
653 $aSperm preparation
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aFREITAS, V. J. de F.
700 1 $aOLIVEIRA, R. V. de
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 45, p. 1431, 2017.
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Embrapa Caprinos e Ovinos (CNPC) |
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