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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/03/2006 |
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Data da última atualização: |
26/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GERMANO, M. G.; MENNA, P.; MOSTASSO, F. L.; HUNGRIA, M. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Reading, v. 56, n. 1, p. 217-229, Jan. 2006. |
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DOI: |
10.1099/ijs.0.02917-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. MenosGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts... Mostrar Tudo |
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Thesagro: |
Fixação de Nitrogênio. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03220naa a2200181 a 4500
001 1468978
005 2024-07-26
008 2006 bl uuuu u00u1 u #d
024 7 $a10.1099/ijs.0.02917-0$2DOI
100 1 $aGERMANO, M. G.
245 $aRFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species.$h[electronic resource]
260 $c2006
520 $aGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined.
650 $aFixação de Nitrogênio
700 1 $aMENNA, P.
700 1 $aMOSTASSO, F. L.
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, Reading$gv. 56, n. 1, p. 217-229, Jan. 2006.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 20 | |
| 3. |  | MORAES, B. S.; SOUZA, A. das G.; CHAGAS, E. A.; SMIDERLE, O. J.; SOUZA, O. M. de. Biometria de sementes, emergência e vigor de plântulas de camu-camu em função da coloração do tegumento. In: ENCONTRO DO PROGRAMA DE INICIAÇÃO CIENTÍFICA, 13., 2014, Boa Vista, RR. Anais... Boa Vista, RR: UFRR, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 4. | | VILENA, J. O.; CHAGAS, E. A.; BACELAR-LIMA. C. G.; ALVES, J. K. B.; SOUZA, O. M. de. Avaliação intraespecífica de acessos de Myrciaria dubia coletados na Serra da Lua, Região do Bonfim. In: ENCONTRO DO PROGRAMA DE INICIAÇÃO CIENTÍFICA, 11., 2012, Boa Vista. Resumos... Boa Vista, RR: UFRR, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 7. | | TAVEIRA, D. L. L.; SOUZA, O. M. de; SIQUEIRA, H. da S.; MELO, V. F.; CHAGAS, E. A. Desenvolvimento de plantas de caçari em resposta a doses de potássio sob condições de campo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 24., 2016, São Luis. Fruticultura: fruteiras nativas e sustentabilidade. São Luis, MA: SBF, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 8. | | SOUZA, O. M. de; BACELAR-LIMA, C. G.; CHAGAS, E. A.; CRUZ, L. de S.; PORTO, W. S. Fenologia da frutificação de populações naturais de camu-camu (Myrciaria dubia (H.B.K.) Mcvaugh) em Roraima. In: ENCONTRO DO PROGRAMA DE INICIAÇÃO CIENTÍFICA, 11., 2012, Boa Vista. Resumos... Boa Vista, RR: UFRR, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 10. | | RIBEIRO, M. I. G.; CHAGAS, E. A.; SANTOS, V. A. dos; OLIVEIRA, J. V. de; SOUZA, O. M. de; RODRIGUEZ, C. R. A. Acúmulo de massa seca na formação de mudas de camu-camu com utilização de diferentes substratos. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 11. | | CHAGAS, E. A.; RIBEIRO, M. I. G.; SOUZA, O. M. de; SANTOS, V. A. de; LOZANO, R. M. B.; BACELAR-LIMA, C. G. Alternatives substrates for production of seedlings camu-camu. Revista de Ciências Agrárias, v. 56, n. Supl., p. 1-7, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Roraima. |
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| 12. | | SOUZA, O. M. de; CHAGAS, E. A.; LOPES, M. T. G.; MAGALHÃES, M. A.; BACELAR-LIMA, C. G.; MORAIS, B. S.; RIBEIRO, M. I. G. Caracterização biométrica de frutos oriundos de populações de taperebazeiro spondias mombin l.(anacardiaceae) da Amazônia Brasileira. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 13. | | BACELAR-LIMA, C. G.; CHAGAS, E. A.; SMIDERLE, O. J.; SOUZA, O. M. de; RIBEIRO, M. I. G.; ALVES, J. K. B. Estudo comparativo da biometria de sementes de camu-camu coletadas em diferentes regiões de Roraima. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 14. | | RIBEIRO, M. I. G.; CHAGAS, E. A.; SANTOS. V. A. dos; OLIVEIRA, J. V. de; SOUZA, O. M. de; RODRIGUEZ, C. R. A. Efeito de diferentes substratos no crescimento e desenvolvimento de porta-enxertos de camu-camu. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 15. | | MORAIS, B. S.; CHAGAS, E. A.; LOZANO, R. M. B.; GRAÇAS, A. de S. das; SOUZA, O. M. de; ALBUQUERQUE, T. C. S. de. Efeito de diferentes concentracões de adubação foliar no desenvolvimento e crescimento de porta-enxertos de camu-camu. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 16. | | RIBEIRO, A. I. G.; CHAGAS, E. A.; SANTOS, V. A. dos; LOZANO, R. M. B.; SOUZA, O. M. de; ARAÚJO, W. F. Efeito da cobertura e substrato na emergência de camu-camu e crescimento de porta-enxerto. In: ENCONTRO DO PROGRAMA DE INICIAÇÃO CIENTÍFICA, 11., 2012, Boa Vista. Resumos... Boa Vista, RR: UFRR, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 17. | | MOTA-FILHO, A. B.; BACELAR-LIMA, C. G.; SOUZA, O. M. de; CHAGAS, E. A.; ARAÚJO, M. da C.; CHAGAS, P. C. Protocolos para germinação e conservação do pólen de camu-camu in vitro. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 18. | | LIMA, H. E. de; SANTOS, V. A. dos; SOUZA, O. M. de; SILVA, D. P. da; FERNANDES, S. B.; CHAGAS, E. A.; ALBUQUERQUE, T. C. S. de. Influência de telas com diferentes cores e malhas sobre a produção de mudas de cupuaçuzeiro. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 19. | | SOUZA, O. M. de; BACELAR-LIMA, C. G.; ALVES, J. K. B.; CHAGAS, E. A.; CRUZ, L. de S.; PORTO, W. S. Fenologia da frutificação de populações naturais de camu-camu (myrciaria dubia (h.b.k.) Mcvaugh) em Roraima. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 20. | | SOUZA, O. M. de; SANTOS, V. A. dos; LIMA, H. E. de; CHAGAS, E. A.; BACELAR-LIMA, C. G.; SOUZA, A. das G.; ALBUQUERQUE, T. C. S. de. Telas de sombreamento no desenvolvimento de mudas de açaízeiro. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| Registros recuperados : 20 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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