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| Registros recuperados : 64 | |
| 6. |  | SILVA, T. D. DA; SOUZA, M, T. DE; SOUZA, M. T. DE; CIPRIANO, R. R.; BIZZO, H. R.; DESCHAMPS, C. Essential Oil Variation in Brazilian Varronia curassavica Jacq. in Response to Drying and Edaphoclimatic Conditions. Journal of Agricultural Science, v. 13, n. 8, 2021. 15 p.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | JANK, L.; SAVIDAN, Y. H.; SOUZA, M. T. de; COSTA, J. C. G. Avaliação do germoplasma de Panicum maximum introduzido da Africa. 1. Produção forrageira. Revista da Sociedade Brasileira de Zootecnia, Viçosa, MG, v.23, n.3, p.433-440, maio/jun. 1994.| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | SOUZA, M. T.; RIBEIRO, R. C.; RODRIGUES, A. L. N.; LEMOS, W. P. Entomofauna presente em plantios de Heliconia spp. e bastão-do-imperador Etlingera elatior no município de Belém, Pará. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 5.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO - 2007), 11., 2008, Belém, PA. Mudanças climáticas globais: sustentabilidade na Amazônia: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2008.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | MOTTA, I. de S.; LEONEL, L. A. K.; PADOVAN, M. P.; SOUZA, M. T. de. Horticultura agroecológica em escala familiar em Mato Grosso do Sul. In: SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 2., 2008, Dourados. A construção participativa da agroecologia em Mato Grosso do Sul: anais. Dourados: Embrapa Agropecuária Oeste, 2008. 1 CD-ROM. Organizado por: Alberto Feiden, Milton Parron Padovan, Adalgiza Inês Campolim, Aurélio Vinícius Borsato, Ivo de Sá Motta, João Batista Catto, Tércio Jacques Fehlauer.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 18. | | SOUZA, M. T.; RIBEIRO, R. C.; RODRIGUES, A. L. N.; LEMOS, W. de P. Principais entomófagos em cultivos de bastão-do-imperador (Eltingera elatior) no nordeste paraense. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida: resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 CD-ROM. SICONBIOL. ID - 159.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SOUZA, M. T.; RIBEIRO, R. C.; RODRIGUES, A. L. N.; LEMOS, W. de P. Principais inimigos naturais em cultivos protegidos de bastão-do-imperador (Etlingera elatior) e helicônia spp. no Município de Belém, Pará. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida: resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 CD-ROM. SICONBIOL. ID - 160.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 64 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/09/2008 |
|
Data da última atualização: |
03/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. |
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Afiliação: |
Ana Flávia Parente, UnB; Ildinete Silva-Pereira, UnB; José Ivo Baldani, Embrapa Agrobiologia; Victor Hugo da Silva Tibúrcio, UnB; Sônia Nair Báo, UnB; Marlene T. de Souza, UnB. |
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Título: |
Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UnB. |
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Conteúdo: |
Collectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein. MenosCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique e... Mostrar Tudo |
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Palavras-Chave: |
Cana-de-açúcar. |
|
Thesagro: |
Bacillus Thuringiensis; Controle Biológico; Diatraea Saccharalis. |
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Categoria do assunto: |
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Marc: |
LEADER 02517naa a2200241 a 4500
001 1598585
005 2009-02-03
008 2008 bl --- 0-- u #d
100 1 $aPARENTE, A. F.
245 $aConstruction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein$btwo potential marker organisms to study bacteria-plant interactions.
260 $c2008
500 $aParceria: UnB.
520 $aCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein.
650 $aBacillus Thuringiensis
650 $aControle Biológico
650 $aDiatraea Saccharalis
653 $aCana-de-açúcar
700 1 $aSILVA-PEREIRA, I.
700 1 $aBALDANI, J. I.
700 1 $aTIBÚRCIO, V. H. da S.
700 1 $aBÁO, S. N.
700 1 $aDE SOUZA, M. T.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 54, p. 786-790, 2008.
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