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Registro Completo |
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
14/11/2005 |
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Data da última atualização: |
27/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COSTA, J. G. da. |
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Afiliação: |
JOAO GOMES DA COSTA, CPAF-AC. |
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Título: |
Correlações entre caracteres do fruto do cupuaçuzeiro. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 15., 1998, Poços de Caldas. Frutas: este mercado vale ouro: resumos. Lavras: Ufla: SBF, 1998. |
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Páginas: |
p. 336. |
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Idioma: |
Português |
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Conteúdo: |
A crescente demanda associada a escassez de conhecimentos requerem o desenvolvimento de pesquisas com a cultura do cupuaçuzeiro. Este trabalho teve como objetivo conhecer as associações existentes entre caracteres do fruto do cupuaçuzeiro, para subsidiar os trabalhos de melhoramento genético da cultura. |
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Palavras-Chave: |
Correlación fenotípica. |
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Thesagro: |
Características Agronômicas; Cupuaçu; Fenótipo; Fruto; Theobroma Grandiflorum. |
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Thesaurus Nal: |
Agronomic traits; Fruits; Phenotypic correlation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/502952/1/11801.pdf
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Marc: |
LEADER 01047nam a2200229 a 4500
001 1502952
005 2024-05-27
008 1998 bl uuuu u00u1 u #d
100 1 $aCOSTA, J. G. da
245 $aCorrelações entre caracteres do fruto do cupuaçuzeiro.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 15., 1998, Poços de Caldas. Frutas: este mercado vale ouro: resumos. Lavras: Ufla: SBF$c1998
300 $ap. 336.
520 $aA crescente demanda associada a escassez de conhecimentos requerem o desenvolvimento de pesquisas com a cultura do cupuaçuzeiro. Este trabalho teve como objetivo conhecer as associações existentes entre caracteres do fruto do cupuaçuzeiro, para subsidiar os trabalhos de melhoramento genético da cultura.
650 $aAgronomic traits
650 $aFruits
650 $aPhenotypic correlation
650 $aCaracterísticas Agronômicas
650 $aCupuaçu
650 $aFenótipo
650 $aFruto
650 $aTheobroma Grandiflorum
653 $aCorrelación fenotípica
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Embrapa Acre (CPAF-AC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA, G. T. de; HELL, R. C. R.; SOUZA, J. F. da S.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Easy in vitro synthesis of optimised functioning reporter mRNA from common eGFP plasmid. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biotechnology, v. 60, n. 10, p. 762-771, 2018. |
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DOI: |
10.1007/s12033-018-0112-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which has been observed as difficult-to-transfect DNA constructs. eGFP expression could be detected both by flow cytometry and by fluorescence microscopy after transfection with the polyadenylated mRNAs. Upon cytometric analysis, we noted a marked difference among the mRNA groups (p < 0.01), both in fluorescent population percentage and in florescence intensity. We showed here the necessity of the polyadenylation step in order to achieve cell expression of the eGFP observable under fluorescence microscopy. The presence of the Kozak sequence, as a 5' element, seems to augment significantly the level of protein produced upon mRNA transfection. We presented here an easy protocol to allow production of functioning mRNAs from any DNA construct. The molecules produced may aid in the standardisation and controlling most of the RNA-related experiments as well as it gives proper guidance for researchers performing expression of other proteins through mRNA transfection. MenosAbstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which ... Mostrar Tudo |
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Palavras-Chave: |
CRISPR/Cas9; EGFP; GFP; Kozak; MRNA; Sequence. |
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Thesagro: |
RNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03181naa a2200253 a 4500
001 2102521
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s12033-018-0112-5$2DOI
100 1 $aSOUZA, G. T. de
245 $aEasy in vitro synthesis of optimised functioning reporter mRNA from common eGFP plasmid.$h[electronic resource]
260 $c2018
520 $aAbstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which has been observed as difficult-to-transfect DNA constructs. eGFP expression could be detected both by flow cytometry and by fluorescence microscopy after transfection with the polyadenylated mRNAs. Upon cytometric analysis, we noted a marked difference among the mRNA groups (p < 0.01), both in fluorescent population percentage and in florescence intensity. We showed here the necessity of the polyadenylation step in order to achieve cell expression of the eGFP observable under fluorescence microscopy. The presence of the Kozak sequence, as a 5' element, seems to augment significantly the level of protein produced upon mRNA transfection. We presented here an easy protocol to allow production of functioning mRNAs from any DNA construct. The molecules produced may aid in the standardisation and controlling most of the RNA-related experiments as well as it gives proper guidance for researchers performing expression of other proteins through mRNA transfection.
650 $aRNA
653 $aCRISPR/Cas9
653 $aEGFP
653 $aGFP
653 $aKozak
653 $aMRNA
653 $aSequence
700 1 $aHELL, R. C. R.
700 1 $aSOUZA, J. F. da S.
700 1 $aCAMARGO, L. S. de A.
773 $tMolecular Biotechnology$gv. 60, n. 10, p. 762-771, 2018.
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