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83. | | GUIMARÃES, C. T.; SILVA, L. da C.; MENDES, F. F.; PASTINA, M. M.; SOUZA, I. R. P. de; DAMASCENO, C. M. B. Mapeamento de QTLs e seleção assistida por marcadores moleculares. In: DELIMA, R. O.; BORÉM, A. (Ed.). Melhoramento de milho. Viçosa, MG: Universidade Federal de Viçosa, 2018. cap. 13, p. 307-328. Biblioteca(s): Embrapa Milho e Sorgo. |
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84. | | RAFAEL, H. A.; SOUZA, I. R. P. de; BARROS, B. A.; PINTO, M. O.; MOREIRA, R. O.; GONÇALVES, I. A. M.; CARVALHO, S. G. M. Molecular detection of the Sugarcane Mosaic Virus causing mosaic disease in maize and sorghum in Brazil. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 94. Biblioteca(s): Embrapa Milho e Sorgo. |
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86. | | RAPOSERIRAS, R.; SOUZA, I. R. P. de; MAGALHAES, J. V. de; GUIMARAES, C. T.; MARRIEL, I. E.; SCHAFFERT, R. E.; ALVES, V. M. C. Mucilage production and differential tolerance to aluminum toxicity in maize (Zea mays L.). In: INTERNATIONAL PLANT NUTRITION COLLOQUIUM, 15., 2005, Beijing, China. Proceedings... Beijing: Tsinghua University, 2005. p. 736-737. Biblioteca(s): Embrapa Milho e Sorgo. |
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87. | | PINTO, M. de O.; SOUZA, I. R. P. de; PAULA, A. L. S. P.; GUIMARAES, P. E. de O.; TRINDADE, R. dos S.; GUIMARAES, L. J. M. Introgressão da resistência ao mosaico-comum em milho assistida por marcadores SNP. In: CONGRESSO NACIONAL DE MILHO E SORGO, 32., 2018, Lavras. Soluções integradas para os sistemas de produção de milho e sorgo no Brasil: resumos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2018. p. 282. Biblioteca(s): Embrapa Milho e Sorgo. |
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90. | | SOUZA, I. R. P. de; GUILHEN, J. H. S.; ANDRADE, C. de L. T. de; PINTO, M. de O.; LANA, U. G. de P.; PASTINA, M. M. Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. Revista Brasileira de Milho e Sorgo, v. 18, n. 3, p. 322-339, 2019. Biblioteca(s): Embrapa Milho e Sorgo. |
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92. | | SOUZA, I. R. P. de; ALVES, V. M. C.; PARENTONI, S. N.; OLIVEIRA, A. C. de; TEIXEIRA, F. F.; ADAM, J. W. Mac. Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 3, p. 219-224, 2002. Biblioteca(s): Embrapa Milho e Sorgo. |
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93. | | MENDES, F. F.; SOUZA, I. R. P. de; MAGALHAES, P. C.; GUIMARAES, L. J. M.; SOUSA, S. M. de; PARENTONI, S. N.; GUIMARAES, C. T. Co-localization of QTLs for root traits, P efficiency indexes and grain yield in maize under low-P soil. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 6., 2015, Brasília. [Resumos]. Brasília, DF: Sociedade Brasileira de Biotecnologia, 2015. Biblioteca(s): Embrapa Milho e Sorgo. |
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94. | | AZEVEDO, T. C. de; TRINDADE, R. dos S.; GUIMARÃES, F. F. M.; LANA, U. G. de P.; GUIMARÃES, S. A.; SOUZA, I. R. P. de. Comparação de protocolos de duplicação cromossômica para a obtenção de duplo-haploides em milho. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/CNPq, 17., 2019, Sete Lagoas. [Trabalhos apresentados]. Sete Lagoas: Embrapa Milho e Sorgo, 2019. Biblioteca(s): Embrapa Milho e Sorgo. |
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96. | | SCHUELTER, A. R.; SOUZA, I. R. P. de; TAVARES, F. F.; SANTOS, M. X. dos; OLIVEIRA, E. de; GUIMARAES, C. T. Controle genético da resistência do milho a mancha por Phaeosphaeria. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 2, n. 1, p. 80-86, jan./abr. 2003. Biblioteca(s): Embrapa Milho e Sorgo. |
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97. | | LOPES, M. J. C.; SOUZA, I. R. P. de; MAGALHAES, P. C.; GAMA, E. E. G. e; ALVES, J. D.; MAGALHAES, M. M. Oxidação protéica e peroxidação lipídica em plantas de diferentes ciclos de seleção do milho 'Saracura', sob encharcamento contínuo. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 4, n. 3, p. 362-373, 2005. Biblioteca(s): Embrapa Milho e Sorgo. |
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98. | | SOUZA, J. B. de; QUEIROZ, C. G. de S.; SOUZA, I. R. P. de; ALVES, V. M. C.; PARENTONI, S. N.; CARNEIRO, N. P.; SCHAFFERT, R. E. Oxidação de proteínas em raízes de milho sob toxidez de alumínio. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 10.; CONGRESSO LATINO AMERICANO DE FISIOLOGIA VEGETAL, 12., 2005, Recife. Anais... Recife: SBFV, 2005. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
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99. | | LOPES, M. J. C.; SOUZA, I. R. P. de; MAGALHAES, P. C.; GAMA, E. E. G. e; ALVES, J. D.; MAGALHAES, M. M.; MARIGUELE, K. H. Peroxidação lipídica foliar em plântulas de diferentes ciclos de seleção do milho 'Saracura' (BRS-4154) sob encharcamento contínuo. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 10.; CONGRESSO LATINO AMERICANO DE FISIOLOGIA VEGETAL, 12., 2005, Recife. Anais... Recife: SBFV, 2005. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
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100. | | SOUZA, J. B.; QUEIROZ, C. G. S.; ISAIAS, R. M. S.; SOUZA, I. R. P. de; PARENTONI, S. N.; SCHAFFERT, R. E.; ALVES, V. M. C. Peroxidação de lipídios de membrana e alterações morfo-anatômicas em raizes de milho sob toxidez de alumínio. Brazilian Journal of Plant Physiology, Campinas, v. 15, p. 383, 2003. Suplemento.
Edição dos resumos do IX Congresso Brasileiro de Fisiologia Vegetal, 2003, Atibaia, SP. Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 158 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/12/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
PURCINO, A. A. C.; LIMA, T. R.; PINTO, A. C.; CARNEIRO, N. P.; SOUZA, I. R. P. de; MARRIEL, I. E.; PARENTONI, S. N.; DURAES, F.; CARVALHO, L. |
Afiliação: |
ANTONIO ALVARO CORSETTI PURCINO, CNPMS; THALES LIMA ROCHA, CENARGEN; NEWTON PORTILHO CARNEIRO, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; Frederico Durães, Embrapa Milho e Sorgo; I. Carvalho, bolsista Embrapa Recursos Genéticos e Biotecnologia. |
Título: |
Glutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Maydica, Bergamo, v. 53, p. 101- 109, 2008. |
Idioma: |
Inglês |
Conteúdo: |
Although nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids. MenosAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Alt... Mostrar Tudo |
Palavras-Chave: |
Glutamine synthetase; Nitrogcn use effi-cicncy; Nitrogen use efficiency; Zea mays L. |
Thesagro: |
Milho; Zea Mays. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 02778naa a2200289 a 4500 001 1491623 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 100 1 $aPURCINO, A. A. C. 245 $aGlutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency.$h[electronic resource] 260 $c2008 520 $aAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids. 650 $aMilho 650 $aZea Mays 653 $aGlutamine synthetase 653 $aNitrogcn use effi-cicncy 653 $aNitrogen use efficiency 653 $aZea mays L 700 1 $aLIMA, T. R. 700 1 $aPINTO, A. C. 700 1 $aCARNEIRO, N. P. 700 1 $aSOUZA, I. R. P. de 700 1 $aMARRIEL, I. E. 700 1 $aPARENTONI, S. N. 700 1 $aDURAES, F. 700 1 $aCARVALHO, L. 773 $tMaydica, Bergamo$gv. 53, p. 101- 109, 2008.
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