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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/02/2013 |
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Data da última atualização: |
24/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PETROLI, C. D.; SANSALONI, C. P; CARLING, J.; STEANE, D. A.; VAILLANCOURT, R. E.; MYBURG, A. M.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; KILIAN, A.; GRATTAPAGLIA, D. |
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Afiliação: |
CESAR D. PETROLI, UNB; CAROLINA P. SANSALONI, UNB; JASON CARLING, DIVERSITY ARRAYS TECHNOLOGY PTY LTD.; DOROTHY A. STEANE, UNIVERSITY OF TASMANIA; RENE E. VAILLANCOURT, UNIVERSITY OF TASMANIA; ALEXANDER A. MYBURG, UNIVERSITY OF PRETORIA; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; ANDRZEJ KILIAN, DIVERSITY ARRAYS TECHNOLOGY PTY LTD.; DARIO GRATTAPAGLIA, CENARGEN. |
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Título: |
Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
PLoS ONE, v. 7, n. 9, set. 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Diversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference genome is yet available to allow such detailed characterization. MenosDiversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in pla... Mostrar Tudo |
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Palavras-Chave: |
Caracterização genómica; Dart genotyping; Genoma do Eucalipto; Microsatellite genotyping. |
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Thesagro: |
Genoma. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02933naa a2200289 a 4500
001 1949877
005 2024-06-24
008 2012 bl uuuu u00u1 u #d
100 1 $aPETROLI, C. D.
245 $aGenomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome.$h[electronic resource]
260 $c2012
520 $aDiversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference genome is yet available to allow such detailed characterization.
650 $aGenoma
653 $aCaracterização genómica
653 $aDart genotyping
653 $aGenoma do Eucalipto
653 $aMicrosatellite genotyping
700 1 $aSANSALONI, C. P
700 1 $aCARLING, J.
700 1 $aSTEANE, D. A.
700 1 $aVAILLANCOURT, R. E.
700 1 $aMYBURG, A. M.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aKILIAN, A.
700 1 $aGRATTAPAGLIA, D.
773 $tPLoS ONE$gv. 7, n. 9, set. 2012.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/03/2024 |
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Data da última atualização: |
20/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COSTA, R. G. B.; PAIXÃO, M. G.; SOUZA, G. N. de; PINTO, S. M.; ABREU, L. R. de. |
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Afiliação: |
RENATA GOLIN BUENO COSTA, EPAMIG; MARCEL GOMES PAIXÃO, EPAMIG; GUILHERME NUNES DE SOUZA, CNPGL; SANDRA MARIA PINTO, UNIVERSIDADE FEDERAL DE LAVRAS; LUIZ RONALDO DE ABREU, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Milk composition and health status of quarters adjacent to an on-farm protocol-treated clinical-mastitis gland. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Animal Production Science, v. 64, AN23176, 2024. |
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DOI: |
https://doi.org/10.1071/AN23176 |
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Idioma: |
Inglês |
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Conteúdo: |
CONTEXT- The hypothesis of interdependency among cows’ mammary glands has been shown, and quarters adjacent to glands with clinical mastitis may have their composition and health status impaired. AIMS- The main focus of this study was to compare foremilk composition (fat, total protein, lactose, solids non-fat (SNF), and chloride) and health status (somatic cell count (SCC), differential leukocytes) of healthy mammary glands adjacent to a gland previously treated (on average, 24 days after treatment, varying from 20 to 39 days) for clinical mastitis with foremilk samples of healthy mammary glands of healthy cows. Our previous study compared these traits (foremilk composition and health status) between healthy mammary glands adjacent to a mastitisinfected quarter and healthy mammary glands from healthy cows (no clinical mastitis) immediately after the identification of mastitis on case quarters. METHODS- The study was designed as a prospective case–control study and the enrolled cows (cases and controls) were matched by days in milk and parity. Case cows (n = 50) were defined as cows that previously had a single quarter infected with mild or moderate clinical mastitis, and which were treated according to an on-farm protocol 1 day after mastitis identification. Control cows (n = 50) were defined as cows that had not suffered clinical mastitis in the current lactation. Foremilk samples from each quarter of case and control cows were collected so as to assess concentrations of fat, total protein, lactose, SCC, and SNF, conduct microbiological analyses, and determine the concentration of chloride and differential leukocyte counts. Multivariate linear regression and logistic regression analyses were performed to assess possible associations of milk-composition and health-status traits between quarter types (case or control cows). KEY RESULTS-On average, 24 days after the treatment of the case quarter began, milk composition (fat, lactose, SNF, and chloride) and some health status (lymphocyte and macrophage counts) of adjacent quarters returned to similar levels as in control quarters, while total protein, SCC, and neutrophils remained greater for adjacent quarters. No effect of quarter position or microbiological results of enrolled quarters were identified. CONCLUSIONS-Our results suggested that at least 20 days (average of 24 days) after an episode of clinical mastitis in a neighbouring gland, adjacent quarters were still recovering from this mastitis episode, and we conclude that interdependency of quarters is a physiological systemic two-way immune response route, and each particular milk component has a different behaviour after clinical mastitis. IMPLICATIONS-Our results suggest that clinical mastitis is far more costly for producers and industry than has been previously reported. Bovine mastitis is the disease that causes the most economical losses to dairy farmers, including veterinary expenses, milk disposal, loss of production and reduction of animal pregnancies. The milk composition of the mammary quarter affected by mastitis is not the only one compromised, because the milk quality of the entire udder is also affected. A period of 20 days is not enough for animals to fully recover from a case of udder inflammation. Mastitis is more costly to producers and industry than has been previously reported. MenosCONTEXT- The hypothesis of interdependency among cows’ mammary glands has been shown, and quarters adjacent to glands with clinical mastitis may have their composition and health status impaired. AIMS- The main focus of this study was to compare foremilk composition (fat, total protein, lactose, solids non-fat (SNF), and chloride) and health status (somatic cell count (SCC), differential leukocytes) of healthy mammary glands adjacent to a gland previously treated (on average, 24 days after treatment, varying from 20 to 39 days) for clinical mastitis with foremilk samples of healthy mammary glands of healthy cows. Our previous study compared these traits (foremilk composition and health status) between healthy mammary glands adjacent to a mastitisinfected quarter and healthy mammary glands from healthy cows (no clinical mastitis) immediately after the identification of mastitis on case quarters. METHODS- The study was designed as a prospective case–control study and the enrolled cows (cases and controls) were matched by days in milk and parity. Case cows (n = 50) were defined as cows that previously had a single quarter infected with mild or moderate clinical mastitis, and which were treated according to an on-farm protocol 1 day after mastitis identification. Control cows (n = 50) were defined as cows that had not suffered clinical mastitis in the current lactation. Foremilk samples from each quarter of case and control cows were collected so as to assess concentrations of f... Mostrar Tudo |
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Palavras-Chave: |
Mastite. |
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Thesagro: |
Doença Animal; Gado Leiteiro; Leite; Produção Leiteira. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 04097naa a2200241 a 4500
001 2163038
005 2024-03-20
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/AN23176$2DOI
100 1 $aCOSTA, R. G. B.
245 $aMilk composition and health status of quarters adjacent to an on-farm protocol-treated clinical-mastitis gland.$h[electronic resource]
260 $c2024
520 $aCONTEXT- The hypothesis of interdependency among cows’ mammary glands has been shown, and quarters adjacent to glands with clinical mastitis may have their composition and health status impaired. AIMS- The main focus of this study was to compare foremilk composition (fat, total protein, lactose, solids non-fat (SNF), and chloride) and health status (somatic cell count (SCC), differential leukocytes) of healthy mammary glands adjacent to a gland previously treated (on average, 24 days after treatment, varying from 20 to 39 days) for clinical mastitis with foremilk samples of healthy mammary glands of healthy cows. Our previous study compared these traits (foremilk composition and health status) between healthy mammary glands adjacent to a mastitisinfected quarter and healthy mammary glands from healthy cows (no clinical mastitis) immediately after the identification of mastitis on case quarters. METHODS- The study was designed as a prospective case–control study and the enrolled cows (cases and controls) were matched by days in milk and parity. Case cows (n = 50) were defined as cows that previously had a single quarter infected with mild or moderate clinical mastitis, and which were treated according to an on-farm protocol 1 day after mastitis identification. Control cows (n = 50) were defined as cows that had not suffered clinical mastitis in the current lactation. Foremilk samples from each quarter of case and control cows were collected so as to assess concentrations of fat, total protein, lactose, SCC, and SNF, conduct microbiological analyses, and determine the concentration of chloride and differential leukocyte counts. Multivariate linear regression and logistic regression analyses were performed to assess possible associations of milk-composition and health-status traits between quarter types (case or control cows). KEY RESULTS-On average, 24 days after the treatment of the case quarter began, milk composition (fat, lactose, SNF, and chloride) and some health status (lymphocyte and macrophage counts) of adjacent quarters returned to similar levels as in control quarters, while total protein, SCC, and neutrophils remained greater for adjacent quarters. No effect of quarter position or microbiological results of enrolled quarters were identified. CONCLUSIONS-Our results suggested that at least 20 days (average of 24 days) after an episode of clinical mastitis in a neighbouring gland, adjacent quarters were still recovering from this mastitis episode, and we conclude that interdependency of quarters is a physiological systemic two-way immune response route, and each particular milk component has a different behaviour after clinical mastitis. IMPLICATIONS-Our results suggest that clinical mastitis is far more costly for producers and industry than has been previously reported. Bovine mastitis is the disease that causes the most economical losses to dairy farmers, including veterinary expenses, milk disposal, loss of production and reduction of animal pregnancies. The milk composition of the mammary quarter affected by mastitis is not the only one compromised, because the milk quality of the entire udder is also affected. A period of 20 days is not enough for animals to fully recover from a case of udder inflammation. Mastitis is more costly to producers and industry than has been previously reported.
650 $aDoença Animal
650 $aGado Leiteiro
650 $aLeite
650 $aProdução Leiteira
653 $aMastite
700 1 $aPAIXÃO, M. G.
700 1 $aSOUZA, G. N. de
700 1 $aPINTO, S. M.
700 1 $aABREU, L. R. de
773 $tAnimal Production Science$gv. 64, AN23176, 2024.
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