Data/hora: 07/06/2024 02:59:41

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 09/06/2021
Data da última atualização: | 09/11/2021
Autoria: | PESSOA, D. D. V.; SANTOS, C. M. dos; VIDAL, M. S.; BALDANI, J. I.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de.
Afiliação: | DANIELLA DUARTE VILLARINHO PESSOA, UFRRJ; CARLOS MAGNO DOS SANTOS, UFRRJ; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB; MICHELLE ZIBETTI TADRA-SFEIR, UFPR; EMANUEL MALTEMPI DE SOUZA, UFPR; JEAN LUIZ SIMOES DE ARAUJO, CNPAB.
Título: | Herbaspirillum seropedicae strain HRC54 expression profile in response to sugarcane apoplastic fluid.
Ano de publicação: | 2021
Fonte/Imprenta: | 3 Biotech, v. 11, Article number 292, 2021.
ISSN: | 2190-5738
DOI: | 10.1007/s13205-021-02848-y
Idioma: | Inglês
Conteúdo: | Bacterial transcriptome profiling in the presence of plant fluids or extracts during microbial growth may provide relevant information on plant?bacteria interactions. Here, RNA sequencing (RNA-Seq) was used to determine the transcriptomic profile of Herbaspirillum seropedicae strain HRC54 at the early stages of response to sugarcane apoplastic fluid. Differentially expressed gene (DEG) analysis was performed using the DESeq2 and edgeR packages, followed by functional annotation using Blast2GO and gene ontology enrichment analysis using the COG and KEGG databases. After 2 h of sugarcane apoplastic fluid addition to the H. seropedicae HRC54 culture, respectively, 44 and 45 genes were upregulated and downregulated. These genes were enriched in bacterial metabolism (e.g., oxidoreductase and transferase), ABC transporters, motility, secretion systems, and signal transduction. RNA-Seq expression profiles of 12 genes identified in data analyses were verified by RT-qPCR. The results suggested that H. seropedicae HRC54 recognized sugarcane apoplastic fluid as the host signal, and some DEGs were closely involved at the early stages of the establishment of plant?bacteria interactions.
Palavras-Chave: | Plant bacteria interactions.
Thesaurus Nal: | Sugarcane.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (41738-1) - UPC - AP - DD -  -  -  - DIS