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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
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Data corrente: |
11/04/2002 |
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Data da última atualização: |
11/04/2002 |
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Autoria: |
MOSTASSO, L.; MOSTASSO, F. L.; DIAS, B. G.; VARGAS, M. A. T.; HUNGRIA, M. |
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Título: |
Selection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 73, p. 121-132, 2002. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
The common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species. MenosThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequen... Mostrar Tudo |
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Palavras-Chave: |
Bean. |
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Thesagro: |
Cerrado; Feijão; Fixação de Nitrogênio; Phaseolus Vulgaris; Rhizobium. |
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Thesaurus Nal: |
nitrogen fixation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02437naa a2200253 a 4500
001 1564655
005 2002-04-11
008 2002 bl --- 0-- u #d
100 1 $aMOSTASSO, L.
245 $aSelection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados.
260 $c2002
520 $aThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species.
650 $anitrogen fixation
650 $aCerrado
650 $aFeijão
650 $aFixação de Nitrogênio
650 $aPhaseolus Vulgaris
650 $aRhizobium
653 $aBean
700 1 $aMOSTASSO, F. L.
700 1 $aDIAS, B. G.
700 1 $aVARGAS, M. A. T.
700 1 $aHUNGRIA, M.
773 $tField Crops Research, Amsterdam$gv. 73, p. 121-132, 2002.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
02/01/2012 |
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Data da última atualização: |
30/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OLIVEIRA G R DE; SILVA M C M da; LUCENA W A; NAKASU E Y T; FIRMINO A A P; BENEVENTI M A; SOUZA D DOS S de L e; GOMES JÚNIOR J E; SOUZA JÚNIOR J D de; RIGDEN D J; RAMOS H B; SOCCOL C R; S M F G de. |
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Afiliação: |
GUSTAVO RAMOS DE OLIVEIRA, CENARGEN; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; WAGNER ALEXANDRE LUCENA, CNPA; ERICH YUKIO TEMPEL NAKASU; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; MAGDA A. BENEVENTI; DJAIR DOS SANTOS DE LIMA E SOUZA, UNB; JOSÉ EDILSON GOMES JÚNIOR; JOSÉ DIJAIR ANTONINO DE SOUZA JÚNIOR, UNB; DANIEL J. RIGDEN; HUDSON B. RAMOS; CARLOS R. SOCCOL; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Biotechnology, 2011. (Open Access) |
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Idioma: |
Francês |
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Palavras-Chave: |
Molecular modeling; Phage display. |
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Thesagro: |
Anthonomus Grandis; Bacillus Thuringiensis. |
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Thesaurus NAL: |
cotton; DNA shuffling. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183405/1/1472-6750-11-85.pdf
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Marc: |
LEADER 00908naa a2200325 a 4500
001 1911334
005 2024-04-30
008 2011 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA G R DE
245 $aImproving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis).$h[electronic resource]
260 $c2011
650 $acotton
650 $aDNA shuffling
650 $aAnthonomus Grandis
650 $aBacillus Thuringiensis
653 $aMolecular modeling
653 $aPhage display
700 1 $aSILVA M C M da
700 1 $aLUCENA W A
700 1 $aNAKASU E Y T
700 1 $aFIRMINO A A P
700 1 $aBENEVENTI M A
700 1 $aSOUZA D DOS S de L e
700 1 $aGOMES JÚNIOR J E
700 1 $aSOUZA JÚNIOR J D de
700 1 $aRIGDEN D J
700 1 $aRAMOS H B
700 1 $aSOCCOL C R
700 1 $aS M F G de
773 $tBMC Biotechnology, 2011. (Open Access)
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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