Data/hora: 22/09/2024 10:40:14

Biblioteca(s): 	Embrapa Rondônia.	
Data corrente: 	13/12/2021	
Data da última atualização: 	14/12/2021	
Autoria: 	SOUZA, C. A. de; ROCHA, R. B.; SANTOS, M. R. A. dos; LOPES, T. A.; TEIXEIRA, A. L.; ESPINDULA, M. C.	
Afiliação: 	CAROLINA AUGUSTO DE SOUZA, Universidade Federal de Rondônia (UNIR); RODRIGO BARROS ROCHA, CPAF-RO; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; TATIANE ALMEIDA LOPES; ALEXSANDRO LARA TEIXEIRA, CPAF-RO; MARCELO CURITIBA ESPINDULA, CPAF-RO.	
Título: 	In vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Crop Breeding and Applied Biotechnology, v. 21, n. 4, e37692142, 2021.	
ISSN: 	1518-7853 (impresso) / 1984-7033 (online)	
DOI: 	http://dx.doi.org/10.1590/1984- 70332021v21n4a51	
Idioma: 	Inglês	
Conteúdo: 	The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic selfincompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.	
Palavras-Chave: 	Café Conilon; In vitro pollination; Microscópio Fluorescente.	
Thesagro: 	Café Robusta; Coffea Canephora; Cultura In Vitro; Genótipo; Polinização; Reprodução Vegetal; Variação Genética.	
Thesaurus Nal: 	Fluorescence microscopy; Genetic variation; Plant reproduction.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/229056/1/cpafro-18641.pdf	
Registro original: 	Embrapa Rondônia (CPAF-RO)	
Exemplares: 	CPAF-RO - (18641-1) - UPC - AP - DD -  -  -  - DIS