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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
05/03/2001 |
Data da última atualização: |
05/03/2001 |
Autoria: |
JACINTO, J. M. M.; IMAÑA-ENCINAS, J. |
Título: |
Análise silvicultural de seis espécies utilizadas na arborização de Brasília. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: CONGRESSO E EXPOSIÇÃO INTERNACIONAL SOBRE FLORESTAS, 6., 2000, Porto Seguro. Resumos técnicos. Rio de Janeiro: Instituto Ambiental Biosfera, 2000. p.11. |
Idioma: |
Português |
Palavras-Chave: |
Analise silvicultural; Brasilia. |
Thesagro: |
Arborização. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00580naa a2200157 a 4500 001 1299812 005 2001-03-05 008 2000 bl uuuu u00u1 u #d 100 1 $aJACINTO, J. M. M. 245 $aAnálise silvicultural de seis espécies utilizadas na arborização de Brasília. 260 $c2000 650 $aArborização 653 $aAnalise silvicultural 653 $aBrasilia 700 1 $aIMAÑA-ENCINAS, J. 773 $tIn: CONGRESSO E EXPOSIÇÃO INTERNACIONAL SOBRE FLORESTAS, 6., 2000, Porto Seguro. Resumos técnicos. Rio de Janeiro: Instituto Ambiental Biosfera, 2000. p.11.
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Embrapa Florestas (CNPF) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
06/11/2017 |
Data da última atualização: |
06/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CARVALHO, M. J. S.; OLIVEIRA, E. J. de; SOUZA, A. da S.; PEREIRA, J. S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de. |
Afiliação: |
M. J. S. CARVALHO; EDER JORGE DE OLIVEIRA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; J. S. PEREIRA; M. S .A. S. DIAMANTINO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
Título: |
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n.2, 2017. |
Série: |
1676-5680 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. MenosThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic ro... Mostrar Tudo |
Thesagro: |
Mandioca. |
Thesaurus NAL: |
cassava. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02559naa a2200217 a 4500 001 2078909 005 2017-11-06 008 2017 bl --- 0-- u #d 100 1 $aCARVALHO, M. J. S. 245 $aCleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture.$h[electronic resource] 260 $c2017 490 $a1676-5680 520 $aThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. 650 $acassava 650 $aMandioca 700 1 $aOLIVEIRA, E. J. de 700 1 $aSOUZA, A. da S. 700 1 $aPEREIRA, J. S. 700 1 $aDIAMANTINO, M. S. A. S. 700 1 $aOLIVEIRA, S. A. S. de 773 $tGenetics and Molecular Research$gv. 16, n.2, 2017.
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