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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/12/2015 |
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Data da última atualização: |
04/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABAKERLI, R. B.; SPARRAPAN, R.; SAWAYA, A. C. H. F.; EBERLIN, M. N.; JARA, J. L. P.; RODRIGUES, N. R.; FAY, E. F.; LUIZ, A. J. B.; GALVÃO, T. D. L.; MARTINS, D. dos S.; YAMANISHI, O. K; TOLEDO, H. H. B. de. |
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Afiliação: |
ROSANGELA BLOTTA ABAKERLI, CPQBA-Unicamp; REGINA SPARRAPAN; ALEXANDRA CHRISTINE HELENA FRANKLAND SAWAYA, IB-Unicamp; MARCOS NOGUEIRA EBERLIN, IQ-UNICAMP; JOSE LUIS PAZ JARA, OQ-UNICAMP; NADIA REGINA RODRIGUES, CPQBA-UNICAMP; ELISABETH FRANCISCONI FAY, CNPMA; ALFREDO JOSE BARRETO LUIZ, CNPMA; TARCILO DAVID LOBO GALVAO, EBDA; DAVID DOS SANTOS MARTINS, INCAPER; OSVALDO KIYOSHI YAMANISHI, UNB; HELOISA HELENA BARRETO DE TOLEDO, IAL. |
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Título: |
Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Food Chemistry, Essex, v. 188, p. 71-76, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC?ITD, were at the average level of 2 mg kg1. |
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Palavras-Chave: |
Benzylisothiocyanate; Dithiocarbamate; Papaya; Residue analysis. |
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Thesagro: |
Carica papaya; Cromatografia; Espectrofotometria; Resíduo químico; Substância tóxica. |
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Thesaurus Nal: |
carbon disulfide; Papayas. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02300naa a2200385 a 4500
001 2030744
005 2023-01-04
008 2015 bl uuuu u00u1 u #d
100 1 $aABAKERLI, R. B.
245 $aCarbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.$h[electronic resource]
260 $c2015
520 $aAbstract: Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC?ITD, were at the average level of 2 mg kg1.
650 $acarbon disulfide
650 $aPapayas
650 $aCarica papaya
650 $aCromatografia
650 $aEspectrofotometria
650 $aResíduo químico
650 $aSubstância tóxica
653 $aBenzylisothiocyanate
653 $aDithiocarbamate
653 $aPapaya
653 $aResidue analysis
700 1 $aSPARRAPAN, R.
700 1 $aSAWAYA, A. C. H. F.
700 1 $aEBERLIN, M. N.
700 1 $aJARA, J. L. P.
700 1 $aRODRIGUES, N. R.
700 1 $aFAY, E. F.
700 1 $aLUIZ, A. J. B.
700 1 $aGALVÃO, T. D. L.
700 1 $aMARTINS, D. dos S.
700 1 $aYAMANISHI, O. K
700 1 $aTOLEDO, H. H. B. de
773 $tFood Chemistry, Essex$gv. 188, p. 71-76, 2015.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
27/02/2019 |
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Data da última atualização: |
27/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, R. C. U.; LARA, L. A. de C.; CHIARI, L.; BARRIOS, S. C. L.; VALLE, C. B. do; VALERIO, J. R.; TORRES, F. Z. V.; GARCIA, A. A. F.; SOUZA, A. P. de. |
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Afiliação: |
Rebecca Caroline Ulbricht Ferreira, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética; Letícia Aparecida de Castro Lara, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; LUCIMARA CHIARI, CNPGC; SANZIO CARVALHO LIMA BARRIOS, CNPGC; CACILDA BORGES DO VALLE, CNPGC; JOSE RAUL VALERIO, CNPGC; FABRICIA ZIMERMANN VILELA TORRES, CNPGC; Antonio Augusto Franco Garcia, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departameto de Genética; Anete Pereira de Souza, Universidade de Campinas - Unicamp/Centro de Biologia Molecular e Engenharia Genética. |
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Título: |
Genetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 10, February, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Urochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle. MenosUrochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies... Mostrar Tudo |
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Palavras-Chave: |
Allele dosage; Linkage map; Signalgrass; SNP. |
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Thesagro: |
Brachiaria. |
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Thesaurus NAL: |
Polyploidy; Quantitative traits. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/193444/1/Genetic-mapping-with-alele-dosage-information.pdf
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Marc: |
LEADER 02764naa a2200301 a 4500
001 2106558
005 2019-02-27
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aGenetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance.$h[electronic resource]
260 $c2019
520 $aUrochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle.
650 $aPolyploidy
650 $aQuantitative traits
650 $aBrachiaria
653 $aAllele dosage
653 $aLinkage map
653 $aSignalgrass
653 $aSNP
700 1 $aLARA, L. A. de C.
700 1 $aCHIARI, L.
700 1 $aBARRIOS, S. C. L.
700 1 $aVALLE, C. B. do
700 1 $aVALERIO, J. R.
700 1 $aTORRES, F. Z. V.
700 1 $aGARCIA, A. A. F.
700 1 $aSOUZA, A. P. de
773 $tFrontiers in Plant Science$gv. 10, February, 2019.
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