| |
|
|
| Registros recuperados : 474 | |
| 82. |  | FONTES, J. M.; MACHADO, C. C.; SOUZA, A. P. de; VIEIRA, L. B.; LORENZONI, R. A. Desenvolvimento de um sistema informatizado para planejamento e controle de manutencao em maquinas florestais: Siplam. Revista Arvore, Vicosa, v.21, n.2, p.279-288, 1997.| Biblioteca(s): Embrapa Florestas. |
|   |
| 83. | | OLIVEIRA, P. B. de; VALLE, C. B. do; SOUZA, A. P.; CANCADO, L. J. Desenvolvimento e caracterização de marcadores moleculares do tipo microssatélites para Brachiaria decumbens. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 8., 2012, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2012. 2 p. (Embrapa Gado de Corte. Documentos, 198).| Biblioteca(s): Embrapa Gado de Corte. |
|  |
| 86. | | SOUZA, A. P.; MACHADO, C. C.; GRIFFITH, J. J.; NEVES, A. R. Controle de impacto ambiental na exploração florestal: proposta de normas. Silvicultura, São Paulo, v. 12, n. 42, t. 32, p. 301-305, 1992. Edição dos Anais do Congresso Florestal Brasileiro, 6., 1990, Campos do Jordão.| Biblioteca(s): Embrapa Florestas. |
| |
| 87. | | VIGNA, B.; JUNGMANN, L.; PAIVA, J.; FRANCISCO, P. M.; VALLE, C. B. do; SOUZA, A. P. Characterization of the parents of a Brachiaria humidicola intraespecific cross that segregates for apomixis, using microsatellites. In: CONGRESSO INTERNACIONAL DE REPRODUÇÃO SEXUAL EM PLANTAS, 20., 2008, Brasília, DF. [Anais...]. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 240 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 259). Capa e conteúdo em inglês. Título em inglês: XX International Congress on Sexual Plant Reproduction. p. 194-195 CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 88. | | SOUZA, A. P. de; SILVA, Z.; SUPERTI, B. F. V.; MARQUES, M. G. Caminhos da Biotecnologia da Reprodução em Suínos. Suinocultura Industrial, Itu, ed. 271, ano 38, n. 04, p. 18-23, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 91. | | SOUSA, A. C. B.; JANK, L.; CAMPOS, T.; SFORÇA, D. A.; JUNGMANN, L.; SOUZA, A. P. Caracterização molecular do banco de germoplasma de Panicum maximum Jacq. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE,5., 2009, Campo Grande, MS. [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 93. | | SOUSA, A. C. B.; BOAVENTURA, L. R.; SILVA, G. M. B.; JUNGMANN, L.; SOUZA, A. P. Caracterização molecular de genótipos tetraplóides selecionados do germoplasma de panicum maximum. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 4., 2008, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2008. 1 CD-ROM. Editores técnicos Valdemir Antônio Laura, Paulo Henrique Nogueira Biscola. 1 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 96. | | CARVALHO, D. F. de; ROCHA, H. S. da; BONOMO, R.; SOUZA, A. P. de. Estimativa da evapotranspiração de referência a partir de dados meteorológicos limitados. PESQUISA AGROPECUÁRIA BRASILEIRA, Brasília, DF, v. 50, n. 1, p.1-11, jan., 2015. Título em inglês: Estimating reference evapotranspiration with limited meteorological data.| Biblioteca(s): Embrapa Solos / UEP-Recife; Embrapa Unidades Centrais. |
| |
| Registros recuperados : 474 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
30/01/2017 |
|
Data da última atualização: |
02/03/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de. |
|
Afiliação: |
REBECCA, C. U. FERREIRA, UNICAMP; LETÍCIA J. CANÇADO; CACILDA BORGES DO VALLE, CNPGC; LUCIMARA CHIARI, CNPGC; ANETE P DE SOUZA, UNICAMP. |
|
Título: |
Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
BMC Research Notes, v. 9, n. 152, p. 1-11, 2016. |
|
Idioma: |
Português |
|
Conteúdo: |
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability MenosBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence ... Mostrar Tudo |
|
Palavras-Chave: |
Enriched library; Transferability. |
|
Thesaurus NAL: |
Forage; Microsatellite repeats; Urochloa brizantha. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154227/1/Microsatellite-loci-for-Urochloa.pdf
|
|
Marc: |
LEADER 02829naa a2200229 a 4500
001 2062128
005 2017-03-02
008 2016 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aMicrosatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species.$h[electronic resource]
260 $c2016
520 $aBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability
650 $aForage
650 $aMicrosatellite repeats
650 $aUrochloa brizantha
653 $aEnriched library
653 $aTransferability
700 1 $aCANÇADO, L. J.
700 1 $aVALLE, C. B. do
700 1 $aCHIARI, L.
700 1 $aSOUZA, A. P. de
773 $tBMC Research Notes$gv. 9, n. 152, p. 1-11, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
