| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
|
Data corrente: |
30/08/2016 |
|
Data da última atualização: |
10/08/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PFEIFER, L. F. M.; SIQUEIRA, L. G. B.; ARASHIRO, E. K. N.; CASTRO, N. A. de; VIANA, J. H. M. |
|
Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL. |
|
Título: |
Prostaglandin F2a or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 6, p. 738-744, jun. 2016. |
|
DOI: |
https://doi.org/10.1590/S0100-204X2016000600005 |
|
Idioma: |
Inglês |
|
Conteúdo: |
ABSTRACT - The objective of this work was to compare two types of ovulation inducers ? estradiol benzoate (EB group) or prostaglandin F2? (PG group) ?, in postpartum dairy cows subjected to timed artificial insemination (TAI). Lactating Holstein?Gir crossbred cows (n=118) with 60 to 120 days of milk production were used in this study. All cows were treated with an intravaginal progesterone?releasing device (CIDR) plus 2 mg of estradiol benzoate (EB) on day 0. The CIDR inserts were removed on day 9, and all cows received 500 ?g sodium cloprostenol. Twenty?four hours after CIDR removal, cows were randomly assigned into two treatment groups and were given either 1 mg of EB i.m. (EB group) or 500 ?g sodium cloprostenol i.m. (PG group). TAI were performed at 52 to 54 hours, in both EB and PG groups, after CIDR removal. No differences were observed in the ovulation (85.2% vs 72.7%) and pregnancy rates (40.7% vs 38.2%) between the EB and PG groups, respectively. Fertility is similar in the TAI protocols that use prostaglandin F2? or EB to induce ovulation in lactating dairy cows. RESUMO - O objetivo deste trabalho foi comparar dois indutores de ovulação – benzoato de estradiol (grupo BE) ou prostaglandina F2α (grupo PG) –, em vacas leiteiras pós‑parto submetidas à inseminação artificial em tempo fixo (IATF). Neste estudo, utilizaram-se vacas Girolando lactantes (n=118) com 60 a 120 dias em lactação. Todas as vacas foram tratadas com um implante intravaginal liberador de progesterona (CIDR) associado a 2 mg de benzoato de estradiol (BE) no dia 0. O CIDR foi retirado no dia 9, e todas as vacas receberam 500 µg i.m. de cloprostenol sódico (análogo à prostaglandina F2α). Vinte e quatro horas após a remoção do CIDR, as vacas foram aleatoriamente separadas em dois grupos, que receberam 1 mg de BE i.m. (grupo BE) ou 500 µg de cloprostenol sódico i.m. (grupo PG). As IATF foram realizadas de 52 a 54 horas, em ambos os grupos, após a remoção do CIDR. Não se observaram diferenças nas taxas de ovulação (85,2 vs. 72,7%) e de prenhez (40,7 vs. 38,2%) entre os grupos BE e PG, respectivamente. A fertilidade é semelhante em protocolos de IATF que utilizam prostaglandina F2α ou BE para induzir a ovulação em vacas leiteiras em lactação. MenosABSTRACT - The objective of this work was to compare two types of ovulation inducers ? estradiol benzoate (EB group) or prostaglandin F2? (PG group) ?, in postpartum dairy cows subjected to timed artificial insemination (TAI). Lactating Holstein?Gir crossbred cows (n=118) with 60 to 120 days of milk production were used in this study. All cows were treated with an intravaginal progesterone?releasing device (CIDR) plus 2 mg of estradiol benzoate (EB) on day 0. The CIDR inserts were removed on day 9, and all cows received 500 ?g sodium cloprostenol. Twenty?four hours after CIDR removal, cows were randomly assigned into two treatment groups and were given either 1 mg of EB i.m. (EB group) or 500 ?g sodium cloprostenol i.m. (PG group). TAI were performed at 52 to 54 hours, in both EB and PG groups, after CIDR removal. No differences were observed in the ovulation (85.2% vs 72.7%) and pregnancy rates (40.7% vs 38.2%) between the EB and PG groups, respectively. Fertility is similar in the TAI protocols that use prostaglandin F2? or EB to induce ovulation in lactating dairy cows. RESUMO - O objetivo deste trabalho foi comparar dois indutores de ovulação – benzoato de estradiol (grupo BE) ou prostaglandina F2α (grupo PG) –, em vacas leiteiras pós‑parto submetidas à inseminação artificial em tempo fixo (IATF). Neste estudo, utilizaram-se vacas Girolando lactantes (n=118) com 60 a 120 dias em lactação. Todas as vacas foram tratadas com um implante intravaginal liberador de progesteron... Mostrar Tudo |
|
Palavras-Chave: |
Período de carência; Sincronização; Withdrawal period. |
|
Thesagro: |
Bovino; Ciclo estral; Inseminação artificial. |
|
Thesaurus Nal: |
Artificial insemination; Cattle; Estrus; Estrus synchronization. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146872/1/Prostaglandin-F2.pdf
|
|
Marc: |
LEADER 03232naa a2200301 a 4500
001 2062868
005 2022-08-10
008 2016 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0100-204X2016000600005$2DOI
100 1 $aPFEIFER, L. F. M.
245 $aProstaglandin F2a or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows.
260 $c2016
520 $aABSTRACT - The objective of this work was to compare two types of ovulation inducers ? estradiol benzoate (EB group) or prostaglandin F2? (PG group) ?, in postpartum dairy cows subjected to timed artificial insemination (TAI). Lactating Holstein?Gir crossbred cows (n=118) with 60 to 120 days of milk production were used in this study. All cows were treated with an intravaginal progesterone?releasing device (CIDR) plus 2 mg of estradiol benzoate (EB) on day 0. The CIDR inserts were removed on day 9, and all cows received 500 ?g sodium cloprostenol. Twenty?four hours after CIDR removal, cows were randomly assigned into two treatment groups and were given either 1 mg of EB i.m. (EB group) or 500 ?g sodium cloprostenol i.m. (PG group). TAI were performed at 52 to 54 hours, in both EB and PG groups, after CIDR removal. No differences were observed in the ovulation (85.2% vs 72.7%) and pregnancy rates (40.7% vs 38.2%) between the EB and PG groups, respectively. Fertility is similar in the TAI protocols that use prostaglandin F2? or EB to induce ovulation in lactating dairy cows. RESUMO - O objetivo deste trabalho foi comparar dois indutores de ovulação – benzoato de estradiol (grupo BE) ou prostaglandina F2α (grupo PG) –, em vacas leiteiras pós‑parto submetidas à inseminação artificial em tempo fixo (IATF). Neste estudo, utilizaram-se vacas Girolando lactantes (n=118) com 60 a 120 dias em lactação. Todas as vacas foram tratadas com um implante intravaginal liberador de progesterona (CIDR) associado a 2 mg de benzoato de estradiol (BE) no dia 0. O CIDR foi retirado no dia 9, e todas as vacas receberam 500 µg i.m. de cloprostenol sódico (análogo à prostaglandina F2α). Vinte e quatro horas após a remoção do CIDR, as vacas foram aleatoriamente separadas em dois grupos, que receberam 1 mg de BE i.m. (grupo BE) ou 500 µg de cloprostenol sódico i.m. (grupo PG). As IATF foram realizadas de 52 a 54 horas, em ambos os grupos, após a remoção do CIDR. Não se observaram diferenças nas taxas de ovulação (85,2 vs. 72,7%) e de prenhez (40,7 vs. 38,2%) entre os grupos BE e PG, respectivamente. A fertilidade é semelhante em protocolos de IATF que utilizam prostaglandina F2α ou BE para induzir a ovulação em vacas leiteiras em lactação.
650 $aArtificial insemination
650 $aCattle
650 $aEstrus
650 $aEstrus synchronization
650 $aBovino
650 $aCiclo estral
650 $aInseminação artificial
653 $aPeríodo de carência
653 $aSincronização
653 $aWithdrawal period
700 1 $aSIQUEIRA, L. G. B.
700 1 $aARASHIRO, E. K. N.
700 1 $aCASTRO, N. A. de
700 1 $aVIANA, J. H. M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 6, p. 738-744, jun. 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
26/12/2019 |
|
Data da última atualização: |
13/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F. |
|
Afiliação: |
Isabella Maiumi Zaidan Blecha, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; Isadora Inácio Sousa, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; ANNA BEATRIZ ROBOTTOM FERREIRA, CTAA; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; FABIANE SIQUEIRA, CNPGC. |
|
Título: |
Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180218, 2019. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. MenosThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform i... Mostrar Tudo |
|
Thesaurus NAL: |
Animal breeding; Marker-assisted selection; Meat tenderness. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207815/1/Alternative-methodologies-for-genotyping.pdf
|
|
Marc: |
LEADER 02589naa a2200217 a 4500
001 2117678
005 2020-01-13
008 2019 bl uuuu u00u1 u #d
100 1 $aBLECHA, I. M. Z.
245 $aAlternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.$h[electronic resource]
260 $c2019
520 $aThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
650 $aAnimal breeding
650 $aMarker-assisted selection
650 $aMeat tenderness
700 1 $aSOUSA, I. I.
700 1 $aFERREIRA, A. B. R.
700 1 $aFEIJO, G. L. D.
700 1 $aTORRES JUNIOR, R. A. de A.
700 1 $aSIQUEIRA, F.
773 $tRevista Brasileira de Zootecnia$gv. 48, e20180218, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
