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| Registros recuperados : 9 | |
| 5. |  | REGO, T. F. C. do; SANTOS, M. P.; CABRAL, G. B.; CIPRIANO, T. de M.; SOUSA, N. L. de; SOUZA NETO, O. A. de; ARAGÃO, F. J. L. Expression of a DREB 5-A subgroup transcription factor gene from Ricinus communis (RcDREB1) enhanced growth, drought tolerance and pollen viability in tobacco. Plant Cell, Tissue and Organ Culture (PCTOC), v. 146, p. 493-504, 2021. P| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | BALDONI, A. B.; CARVALHO, M. H. DE.; SOUSA, N. L.; NOBREGA, M. B. de M.; MILANI, M.; ARAGÃO, F. J. L. Variability of ricin content in mature seeds of castor bean. Pesquisa Agropecuária Brasileira, v. 46, n. 7, p. 776-779, jul., 2011.| Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 7. | | GOMES JUNIOR, R. A.; BOARI, A. de J.; PEIXOTO, M. A. de A.; BENCHIMOL, R. L.; CUNHA, B. P. de O.; SOUSA, N. L. de; SILVA, J. P. A. da; SILVA, R. T. da; SOUSA, E. R. M. de. Seleção preliminar de linhagens de segundo ciclo de melhoramento de feijão-caupi do tipo manteiguinha na Amazônia Oriental. Belém, PA: Embrapa Amazônia Oriental, 2024. 17 p. (Embrapa Amazônia Oriental. Boletim de pesquisa e desenvolvimento, 164).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | BUCARETCHI, F.; BORRASCA-FERNANDES, C. F.; PRADO, C. C.; LANARO, R.; COSTA, J. L.; PETRONI, O. M.; GIRALDI, T.; BLOTTA, M. H. S. L.; JUSTO-JUNIOR, A. S.; SOUSA, N. L.; ARAGÃO, F. J. L.; CAPITANI, E. M. de; HYSLOP, S. Near-fatal poisoning after ricin injection. Clinical Toxicology, v. 59, n. 2, p. 158-168, 2021.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrossilvipastoril. Para informações adicionais entre em contato com cpamt.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
06/02/2018 |
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Data da última atualização: |
06/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUSA, N. L.; CABRAL, G. B.; VIEIRA, P. M.; BALDONI, A. B.; ARAGAO, F. J. L. |
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Afiliação: |
NATÁLIA L. SOUSA, UNB; GLAUCIA BARBOSA CABRAL, Cenargen; PABLINE M. VIEIRA, INSTITUTO FEDERAL GOIANO; AISY BOTEGA BALDONI TARDIN, CPAMT; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
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Título: |
Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientific Reports, v. .7, n. 1, 2017. (Open). |
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DOI: |
10.1038/s41598-017-15636-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. |
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Palavras-Chave: |
Toxic ribosome-inactivating. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01828naa a2200193 a 4500
001 2087235
005 2018-02-06
008 2017 bl --- 0-- u #d
024 7 $a10.1038/s41598-017-15636-7$2DOI
100 1 $aSOUSA, N. L.
245 $aBio-detoxification of ricin in castor bean (Ricinus communis L.) seeds.$h[electronic resource]
260 $c2017
520 $aRicin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society.
653 $aToxic ribosome-inactivating
700 1 $aCABRAL, G. B.
700 1 $aVIEIRA, P. M.
700 1 $aBALDONI, A. B.
700 1 $aARAGAO, F. J. L.
773 $tScientific Reports$gv. .7, n. 1, 2017. (Open).
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