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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
29/09/1998 |
Data da última atualização: |
29/09/1998 |
Autoria: |
EUCLIDES FILHO, K.; FIGUEIREDO, G. R. de; ALVES, R. G. de O.; SILVA, L. O. C. da. |
Afiliação: |
EMBRAPA. Centro Nacional de Pesquisa de Gado de Corte (Campo Grande, MS). |
Título: |
Estimativas de efeitos geneticos aditivos direto e materno em bovinos mesticos. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 35., 1998, Botucatu. Anais... Botucatu: SBZ, 1998. |
Volume: |
v.3. |
Páginas: |
p.467-469. |
Idioma: |
Português |
Notas: |
CNPGC. |
Conteúdo: |
Foram ulilizadas informacoes referentes a pesos a desmama de 883 animais oriundos de seis grupos de acasalamentos, e 10 touros/raca, envolvendo cruzamentos de entre animais das racas Simental, Charoles e Chianina com a raca Nelore, retrocruzamentos e Nelore puro. Os pesos a desmama foram ajustados para as idades do bezerro e da mae, e para grupo contemporaneo (constituido pela combinacao de ano e estacao de nascimento e sexo) a fim de se estimarem os efeitos geneticos direto e materno sobre o peso a desmama. Os efeitos aditivos foram -4,43 kg para a raca Simental, -6,58 kg para a Chianina e -15,24 kg para a raca Charoles. Os efeitos maternos, por outro lado, foram -41,78 kg para a raca Simental, -26,50 kg para Charoles e -26,11 kg para a raca Chianina. |
Palavras-Chave: |
Bovino de corte; Charoles; Crossbreeding; Nelore; Simental. |
Thesagro: |
Cruzamento; Desmama; Melhoramento; Peso. |
Thesaurus Nal: |
beef cattle; breeding; Charolais; Chianina; Nellore; Simmental; weaning weight. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01780naa a2200385 a 4500 001 1318204 005 1998-09-29 008 1998 bl uuuu u00u1 u #d 100 1 $aEUCLIDES FILHO, K. 245 $aEstimativas de efeitos geneticos aditivos direto e materno em bovinos mesticos. 260 $c1998 300 $ap.467-469. v.3. 490 $vv.3. 500 $aCNPGC. 520 $aForam ulilizadas informacoes referentes a pesos a desmama de 883 animais oriundos de seis grupos de acasalamentos, e 10 touros/raca, envolvendo cruzamentos de entre animais das racas Simental, Charoles e Chianina com a raca Nelore, retrocruzamentos e Nelore puro. Os pesos a desmama foram ajustados para as idades do bezerro e da mae, e para grupo contemporaneo (constituido pela combinacao de ano e estacao de nascimento e sexo) a fim de se estimarem os efeitos geneticos direto e materno sobre o peso a desmama. Os efeitos aditivos foram -4,43 kg para a raca Simental, -6,58 kg para a Chianina e -15,24 kg para a raca Charoles. Os efeitos maternos, por outro lado, foram -41,78 kg para a raca Simental, -26,50 kg para Charoles e -26,11 kg para a raca Chianina. 650 $abeef cattle 650 $abreeding 650 $aCharolais 650 $aChianina 650 $aNellore 650 $aSimmental 650 $aweaning weight 650 $aCruzamento 650 $aDesmama 650 $aMelhoramento 650 $aPeso 653 $aBovino de corte 653 $aCharoles 653 $aCrossbreeding 653 $aNelore 653 $aSimental 700 1 $aFIGUEIREDO, G. R. de 700 1 $aALVES, R. G. de O. 700 1 $aSILVA, L. O. C. da 773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 35., 1998, Botucatu. Anais... Botucatu: SBZ, 1998.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
19/04/2023 |
Data da última atualização: |
19/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
DOI: |
http://dx.doi.org/10.4238/gmr19118 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
Thesagro: |
Diagnostico. |
Thesaurus NAL: |
Senecavirus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500 001 2153239 005 2023-04-19 008 2023 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI 100 1 $aRIEGER, J. S. G. 245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource] 260 $c2023 520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. 650 $aSenecavirus 650 $aDiagnostico 700 1 $aMANTOVANI, C. 700 1 $aSUNIGA, P. A. P. 700 1 $aPINTO, I. B. 700 1 $aSOUSA, G. A. A. 700 1 $aGAVA, D. 700 1 $aCANTAO, M. E. 700 1 $aSANTOS, L. R. 700 1 $aARAÚJO, F. R. 700 1 $aZANELLA, J. R. C. 773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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