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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/10/1993 |
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Data da última atualização: |
06/10/1993 |
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Autoria: |
JARRET, R. L.; VUYLSTEKE, D. R.; GAWEL, N. J.; PIMENTEL, R. B.; DUNBAR, L. J. |
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Afiliação: |
United States Department of Agriculture, Agricultural Research Service, Regional Plant Introduction Station, 1109 Experiment Street, Griffin, GA 30223 USA (address for offprints). |
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Título: |
Detecting genetic diversity in diploid bananas using PCR and primers from a highly respectitive DNA sequence. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Euphytica International Journal of Plant Breeding, v.68, n.1-2, 1993. |
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ISSN: |
0014-2336 |
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Idioma: |
Inglês |
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Conteúdo: |
The polymerase chain reaction (PCR) was used to detect polymorphisms among 29 diploid clones of Musa acuminata Colla. from Papua New Guinea. Primer sequences were derived from a 520 bp highly respectitive DNA sequence isolated from M.acuminata ssp. malaccensis. Primers, used individually, detected a total of 48 polymorphisms that were scored as unit characters and used to generate a Jaccard's similarity index. Principal coordinate analysis (PCO) was used to cluster clones and the unweighted paired-group method of analysis (UPGMA) was used to compute genetic distance among the materials examined. The abundance of diversity within the PNG diploids examined reflects the exterme genetic variability within the M.acuminata gene pool. PCR,utilizing primes from a highly respetitive sequence, is a rapid means of detecting genetic diversity in M. acuminata. |
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Palavras-Chave: |
Diploid banana; PCR; Plant germplasm; Repetitive sequence. |
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Thesagro: |
Musa Acuminata. |
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Thesaurus Nal: |
Papua New Guinea; polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01636naa a2200265 a 4500
001 1632831
005 1993-10-06
008 1993 bl uuuu u00u1 u #d
022 $a0014-2336
100 1 $aJARRET, R. L.
245 $aDetecting genetic diversity in diploid bananas using PCR and primers from a highly respectitive DNA sequence.
260 $c1993
520 $aThe polymerase chain reaction (PCR) was used to detect polymorphisms among 29 diploid clones of Musa acuminata Colla. from Papua New Guinea. Primer sequences were derived from a 520 bp highly respectitive DNA sequence isolated from M.acuminata ssp. malaccensis. Primers, used individually, detected a total of 48 polymorphisms that were scored as unit characters and used to generate a Jaccard's similarity index. Principal coordinate analysis (PCO) was used to cluster clones and the unweighted paired-group method of analysis (UPGMA) was used to compute genetic distance among the materials examined. The abundance of diversity within the PNG diploids examined reflects the exterme genetic variability within the M.acuminata gene pool. PCR,utilizing primes from a highly respetitive sequence, is a rapid means of detecting genetic diversity in M. acuminata.
650 $aPapua New Guinea
650 $apolymerase chain reaction
650 $aMusa Acuminata
653 $aDiploid banana
653 $aPCR
653 $aPlant germplasm
653 $aRepetitive sequence
700 1 $aVUYLSTEKE, D. R.
700 1 $aGAWEL, N. J.
700 1 $aPIMENTEL, R. B.
700 1 $aDUNBAR, L. J.
773 $tEuphytica International Journal of Plant Breeding$gv.68, n.1-2, 1993.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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