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| 3. |  | FRANÇA, A. M. S.; SANO, E. E.; SOUSA, A. O. Avaliação de fusão de imagens CBERS/2B no mapeamento de áreas úmidas do Distrito Federal. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 155-156 (Embrapa Cerrados. Documentos, 243).| Biblioteca(s): Embrapa Cerrados. |
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| 6. | | ORNELLAS, F. L. S.; DANTAS, B. F.; COSTA, D. S.; SOUSA, A. O.; BARBOSA, R. M. Teste de vigor baseado na liberação de etanol em sementes de melão. Informativo ABRATES, Londrina, v. 27, n. 2, p. 204, ago. 2017. Número especial. Edição dos Resumos do XX Congresso Brasileiro de Sementes, Foz do Iguaçu, ago. 2017.| Biblioteca(s): Embrapa Semiárido. |
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| 7. | | SOUSA, A. O.; RODRIGUES, B. A.; NARCISO, M. G.; MENESES, M. V.; GUIMARAES, C. M. Software de obtenção do Índice de Vegetação por Diferença Normalizada. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 94. (Embrapa Arroz e Feijão. Documentos, 309).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | ALMEIDA, A. P.; ANDRIOLI, A.; SOUSA, A. O.; MACIEL, T. T. Superovulação em caprinos nativos da raça Moxotó. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 31., 2004, São luis. A medicina veterinária no novo milênio: transformação social, preservação ambiental e segurança alimentar: resumos. São Luis, Sociedade Brasileira de Medicina Veterinária, 2004. Seção biotecnologia da reprodução. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | ORNELLAS, F. L. S.; SOUSA, A. O. de; PIROVANI, C. P.; ARAÚJO, M. do N; COSTA, D. S. da; DANTAS, B. F.; BARBOSA, R. M. Gene expression, biochemical and physiological activities in evaluating melon seed vigor through ethanol release. Scientia Horticulturae, v. 261, 108884, 2020.| Biblioteca(s): Embrapa Semiárido. |
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| 12. | | GONÇALVES, L. P.; ALVES, T. F. O.; MARTINS, C. P. S.; SOUSA, A. O.; GIRARDI, E. A.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Analysis of oxidative damage in varieties of Citrus rootstocks subjected to water deficit. WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [S.l.]: International Advanced Biology Consortium, 2013.Oline. S02001.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". Plos One, July, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios. BMC Plant Biology, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | GONÇALVES, L. P.; ALVES, T. F. O.; MARTINS, C. P. S.; SOUSA, A. O. de; SANTOS, I. C. dos; PIROVANI, C. P.; ALMEIDA, A-A. F.; COELHO FILHO, M. A.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; GIRARDI, E. A.; COSTA, M. G. C. Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange. Acta Physiologiae Plantarum, June, .38- 174, 2016. 0137-5881| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/07/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. |
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Afiliação: |
SARA PEREIRA MENEZES, UESC; DSON MARIO DE ANDRADE SILVA, UESC; ELINE MATOS LIMA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; BRUNO SILVA ANDRADE; LIVIA SANTOS LIMA LEMOS; ARINA PERES GRAMACHO; ABELMON DA SILVA GESTEIRA, CNPMF; CARLOS PRIMINHO PIROVANI; FABIENNE MICHELI, UESC. |
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Título: |
The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Plant Biology, 2014. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. MenosBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first rep... Mostrar Tudo |
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Thesagro: |
Cacau; Theobroma Cacao. |
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Categoria do assunto: |
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Marc: |
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100 1 $aMENEZES, S. P.
245 $aThe pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios.$h[electronic resource]
260 $c2014
520 $aBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively.
650 $aCacau
650 $aTheobroma Cacao
700 1 $aSILVA, D. M. de A.
700 1 $aLIMA, E. M.
700 1 $aSOUSA, A. O. de
700 1 $aANDRADE, B. S.
700 1 $aLEMOS, L. S. L.
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700 1 $aGESTEIRA, A. da S.
700 1 $aPIROVANI, C. P.
700 1 $aMICHELI, F.
773 $tBMC Plant Biology, 2014.
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