Data/hora: 25/06/2024 00:59:07

Biblioteca(s): 	Embrapa Agroindústria Tropical; Embrapa Caprinos e Ovinos.	
Data corrente: 	22/03/2023	
Data da última atualização: 	27/11/2023	
Autoria: 	SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAUJO, J. F.; PEIXOTO, R. M.; AZEVEDO, D. A. A. de; LIMA, A. M. C.; CANUTO, K. M.; RIBEIRO, P. R. V.; SOUZA, A. S. de Q.; SOUZA, S. C. R.; AMORIM, S. L. de; AMARAL, G. P.; SOUZA, V. de; MORAIS, S. M. de; ANDRIOLI, A.; TEIXEIRA, M. F. da S.	
Afiliação: 	ANA LIDIA MADEIRA DE SOUSA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE); RAYMUNDO RIZALDO PINHEIRO, CNPC; JUSCILANIA FURTADO ARAUJO, University Center INTA (UNINTA), Tianguá, CE, Brazil; RENATO MESQUITA PEIXOTO, Vale do Salgado University Center (UNIVS) - Icó, CE, Brazil; Terra Nordeste College (FATENE) - Caucaia, CE, Brazil; DALVA ALANA ARAGAO DE AZEVEDO; ANA MILENA CESAR LIMA, National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Embrapa; KIRLEY MARQUES CANUTO, CNPAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; ANA SHEILA DE QUEIROZ SOUZA; SAMARA CRISTINA ROCHA SOUZA; SARA LUCENA DE AMORIM; GABRIEL PAULA AMARAL, State University of Acaraú Valley - Sobral, CE, Brazil; VIVIANE DE SOUZA, CNPC; SELENE MAIA DE MORAIS, Laboratory of Chemistry and Natural Products (LQPN), Ceará State University, Fortaleza, CE, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; MARIA FATIMA DA SILVA TEIXEIRA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE), Fortaleza, CE, Brazil.	
Título: 	In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Scientific Reports, v. 13, article 4677, Mar. 2023.	
DOI: 	https://doi.org/10.1038/s41598-023-31455-5	
Idioma: 	Inglês	
Conteúdo: 	Abstract: This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.	
Palavras-Chave: 	Antiviral plants; CAEV; Caprine Lentivirus; CLV; Composto bioativo; Ethanolic extract; Extrato de plantas; SRLV.	
Thesagro: 	Azadirachta Indica; Extrato Vegetal; Fitoterapia; Melia Azedarach; Nim; Planta Medicinal.	
Thesaurus Nal: 	Antiviral properties; Goat diseases; Medicinal plants; Phytotherapy; Plant extracts; Sheep diseases.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (40460-1) - UPC - AP - DD -  -  -  - DIS
CNPAT - (17059-1) - UPC - AP - DD -  -  -  - DIS