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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
03/10/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOREIRA, V. J. V; LOURENCO, I. T.; BASSO, M. F.; SÁ. M. E. L. de; MORGANTE, C. V.; MELO, B. P.; ARRAES, F. B. M.; SÁ, D. M. de; SILVA, M. C. M. da; ENGLER, J. de A.; SA, M. F. G. de. |
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Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, UnB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology - INCT; MARIA EUGÊNIA LISEI DE SA, UnB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, UFV; FABRÍCIO BARBOSA MONTEIRO ARRAES, National Institute of Science and Technology - INCT; DIOGO MARTINS DE SA, UnB; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology - INCT; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Minc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Planta, v. 255, n. 2, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Minc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field. MenosMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 ge... Mostrar Tudo |
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Palavras-Chave: |
Interação planta-nematóide; Minc03328; Nematoide das galhas; Novas ferramentas biotecnológica; Planta RNAi; Proteção de cultivos; Proteína efetora. |
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Thesagro: |
Meloidogyne Incognita; Nematóide; Planta. |
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Thesaurus Nal: |
Plants (botany); Root-knot nematodes. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147069/1/Minc03328-effector-gene-downregulation-2022.pdf
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Marc: |
LEADER 03276naa a2200385 a 4500
001 2147069
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
100 1 $aMOREIRA, V. J. V
245 $aMinc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana.$h[electronic resource]
260 $c2022
520 $aMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field.
650 $aPlants (botany)
650 $aRoot-knot nematodes
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aPlanta
653 $aInteração planta-nematóide
653 $aMinc03328
653 $aNematoide das galhas
653 $aNovas ferramentas biotecnológica
653 $aPlanta RNAi
653 $aProteção de cultivos
653 $aProteína efetora
700 1 $aLOURENCO, I. T.
700 1 $aBASSO, M. F.
700 1 $aSÁ. M. E. L. de
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P.
700 1 $aARRAES, F. B. M.
700 1 $aSÁ, D. M. de
700 1 $aSILVA, M. C. M. da
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 255, n. 2, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
14/05/2001 |
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Data da última atualização: |
21/11/2018 |
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Autoria: |
MACHADO, A. T.; SODEK, L.; FERNANDES, M. S. |
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Afiliação: |
ALTAIR TOLEDO MACHADO, CPAC; Ladaslav Sodek, Universidade Estadual de Campinas - Unicamp/Departamento de Fisiologia Vegetal; Mânlio Silvestre Fernandes, Universidade Federal Rural do Rio de Janeiro - UFRRJ/Departamento de Solos. |
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Título: |
N-partitioning, nitrate reductase and glutamine synthetase activities in two contrasting varieties of maize. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 2, p. 249-256, fev. 2001 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Partição de nitrogênio e atividade das enzimas nitrato redutase e glutamina sintetase em duas cultivares contrastantes de milho. |
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Conteúdo: |
In order to identify useful parameters for maize genetic breeding programs aiming at a more efficient use of N, two maize varieties of contrasting N efficiency, Sol da Manhã NF (efficient) and Catetão (inefficient) were compared. Experiments were carried out under field and greenhouse conditions, at low and high N levels. The parameters analysed included total and relative plant and grain N content, biomass and the activities of nitrate reductase and glutamine synthetase in different parts of the plant. It was found that the translocation efficiency of N and photoassimilates to the developing seeds and the source-sink relations were significantly different for the two varieties. N content of the whole plant and grain, cob weight and the relative ear dry weight were useful parameters for characterizing the variety Sol da Manhã NF as to its efficient use of N. Enzymes activity of glutamine synthetase (transferase reaction) and nitrate reductase did not differ among the varieties. |
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Palavras-Chave: |
Melhoramento de planta; Relação fonte-dreno. |
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Thesagro: |
Grão; Melhoramento Genético Vegetal; Milho; Nitrogênio; Transporte de Nutriente. |
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Thesaurus NAL: |
Corn; Nitrogen; Nutrient transport; Plant breeding. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/18933/1/pab99_230.pdf
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Marc: |
LEADER 01990naa a2200289 a 4500
001 1103665
005 2018-11-21
008 2001 bl uuuu u00u1 u #d
100 1 $aMACHADO, A. T.
245 $aN-partitioning, nitrate reductase and glutamine synthetase activities in two contrasting varieties of maize.
260 $c2001
500 $aTítulo em português: Partição de nitrogênio e atividade das enzimas nitrato redutase e glutamina sintetase em duas cultivares contrastantes de milho.
520 $aIn order to identify useful parameters for maize genetic breeding programs aiming at a more efficient use of N, two maize varieties of contrasting N efficiency, Sol da Manhã NF (efficient) and Catetão (inefficient) were compared. Experiments were carried out under field and greenhouse conditions, at low and high N levels. The parameters analysed included total and relative plant and grain N content, biomass and the activities of nitrate reductase and glutamine synthetase in different parts of the plant. It was found that the translocation efficiency of N and photoassimilates to the developing seeds and the source-sink relations were significantly different for the two varieties. N content of the whole plant and grain, cob weight and the relative ear dry weight were useful parameters for characterizing the variety Sol da Manhã NF as to its efficient use of N. Enzymes activity of glutamine synthetase (transferase reaction) and nitrate reductase did not differ among the varieties.
650 $aCorn
650 $aNitrogen
650 $aNutrient transport
650 $aPlant breeding
650 $aGrão
650 $aMelhoramento Genético Vegetal
650 $aMilho
650 $aNitrogênio
650 $aTransporte de Nutriente
653 $aMelhoramento de planta
653 $aRelação fonte-dreno
700 1 $aSODEK, L.
700 1 $aFERNANDES, M. S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 36, n. 2, p. 249-256, fev. 2001
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