Data/hora: 21/06/2024 15:04:37

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 21/05/2015
Data da última atualização: | 18/05/2023
Autoria: | SOARES, T. L.; SOUZA, E. H. de; SAMPAIO, L. F. S.; COSTA, M. A. P. de C.; SILVA, S. de O. e; SEREJO, J. A. dos S.
Afiliação: | TALIANE LEILA SOARES; EVERTON HILO DE SOUZA; LAECIO FERNANDES SOUZA SAMPAIO; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA; SEBASTIÃO DE OLIVEIRA E SILVA; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF.
Título: | Effect of collection time on the viability of banana pollen grains.
Ano de publicação: | 2015
Fonte/Imprenta: | African Journal of Biotechnology, v.14, n. 4, p.1207-1214, April, 2015.
ISSN: | 1684-5315
Idioma: | Inglês
Conteúdo: | The objective of this study was to assess the pollen viability of six improve ddiploid banana plants (AA) collected in different periods of the day , in two seasons of the year (winter and summer), usingin vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10am, 12pm, 2pm and 4pm were evaluated . We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%KNO3, 0.03%Ca(NO3)2.4H2O, and 0.02%MgSO4.7H2O, solidified with 0.8% agar, adjusted todifferent pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride(TTC).The highest pollen germination rates and viability were obtained at 8amand the lowest at 4pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer,the pollen viability and in vitrogermination rates werehighest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollencollection time for use in cross breedingprograms for plant improvemen.
Thesagro: | Banana.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (30669-1) - UPC - AP - DD - Publicação digital -  -  - DIS