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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
17/12/2018 |
Data da última atualização: |
17/12/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R. |
Afiliação: |
Juliana Santos Souza, Universidade Anhanguera - Uniderp/Campus Agrárias; LUCIMARA CHIARI, CNPGC; ROSANGELA MARIA SIMEAO, CNPGC; MARIANE DE MENDONCA VILELA, CNPGC; Leonardo Rippel Salgado, CNPGC. |
Título: |
Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
American Journal of Plant Science, v. 18, n. 3, p. 314-319, July/Sept. 2018. |
DOI: |
DOI: 10.4236/ajps.2018.92023 |
Idioma: |
Inglês |
Conteúdo: |
Urochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species. MenosUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are th... Mostrar Tudo |
Palavras-Chave: |
Signalgrass; Simple Sequence Repeat; Transferability. |
Thesagro: |
Brachiaria. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188572/1/8-Development-validation-and-characterization.pdf
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Marc: |
LEADER 02299naa a2200229 a 4500 001 2101685 005 2018-12-17 008 2018 bl uuuu u00u1 u #d 024 7 $aDOI: 10.4236/ajps.2018.92023$2DOI 100 1 $aSOUZA, J. S. 245 $aDevelopment, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species.$h[electronic resource] 260 $c2018 520 $aUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species. 650 $aBrachiaria 653 $aSignalgrass 653 $aSimple Sequence Repeat 653 $aTransferability 700 1 $aCHIARI, L. 700 1 $aRESENDE, R. M. S. 700 1 $aVILELA, M. de M. 700 1 $aSALGADO, L. R. 773 $tAmerican Journal of Plant Science$gv. 18, n. 3, p. 314-319, July/Sept. 2018.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
29/04/2009 |
Data da última atualização: |
29/04/2009 |
Tipo da produção científica: |
Artigo de Divulgação na Mídia |
Autoria: |
SOARES, D. M. |
Afiliação: |
Dino Magalhães Soares, CNPAF. |
Título: |
Produtores das várzeas do Tocantins objetivam a Indicação Geográfica (IG). |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: TOCANTINS. Secretaria da Agricultura, Pecuária e Abastecimento. Disponível em: . Acesso em: 6 fev.2009. |
Idioma: |
Português |
Conteúdo: |
Cerca de 38 produtores das várzeas tocantinenses, com apoio de várias entidades, criaram a Associação dos Produtores de Sementes e mudas da Lagoa da Confusão e várzeas do Tocantins - APROSEL - TO. A conquista do selo de identificação geográfica (IG) é um dos objetivos da APROSEL. |
Palavras-Chave: |
Origem geográfica; Tocantins. |
Thesagro: |
Identificação; Semente; Sistema de Produção. |
Categoria do assunto: |
-- |
URL: |
https://seagro.to.gov.br/noticia.php?id=1076
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Marc: |
LEADER 00905naa a2200181 a 4500 001 1217013 005 2009-04-29 008 2009 bl --- 0-- u #d 100 1 $aSOARES, D. M. 245 $aProdutores das várzeas do Tocantins objetivam a Indicação Geográfica (IG). 260 $c2009 520 $aCerca de 38 produtores das várzeas tocantinenses, com apoio de várias entidades, criaram a Associação dos Produtores de Sementes e mudas da Lagoa da Confusão e várzeas do Tocantins - APROSEL - TO. A conquista do selo de identificação geográfica (IG) é um dos objetivos da APROSEL. 650 $aIdentificação 650 $aSemente 650 $aSistema de Produção 653 $aOrigem geográfica 653 $aTocantins 773 $tIn: TOCANTINS. Secretaria da Agricultura, Pecuária e Abastecimento. Disponível em: <http://seagro.to.gov.br/noticia.php?id=1076>. Acesso em: 6 fev.2009.
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Embrapa Arroz e Feijão (CNPAF) |
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