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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
23/11/2018 |
Data da última atualização: |
18/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PINHEIRO, D. H.; VÉLEZ, A. M.; FISHILEVICH, E.; WANG, H.; CARNEIRO, N. P.; VALENCIA-JIMÉNEZ, A.; VALICENTE, F. H.; NARVA, E. K.; SIEGFRIED, B. D. |
Afiliação: |
Daniele H. Pinheiro, Universidade Federal de Lavras; Ana M. Vélez, University of Lincoln; Elane Fishilevich, University of Lincoln; Haichuan Wang, University of Lincoln; NEWTON PORTILHO CARNEIRO, CNPMS; Arnubio Valencia-Jiménez, Universidad de Caldas, Colombia; FERNANDO HERCOS VALICENTE, CNPMS; Kenneth E. Narva, DowDuPont; Blair D. Siegfried, University of Florida. |
Título: |
Clathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeCont. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Plos One, v. 13, n. 8, e0201849, Aug. 2018. |
DOI: |
10.1371/journal.pone.0201849 |
Idioma: |
Inglês |
Conteúdo: |
The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H+ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led to a significant decrease in the effects of laccase2 dsRNA reporter, indicating that these genes are involved in RNAi response. However, the knockdown of either Arf72A or Rab7 did not suppress the response to laccase2 dsRNA. The silencing of the silC gene did not lead to a significant reduction in mortality or increase in the expression of V-ATPase A reporter. While the silencing of the silA gene significantly decreased insect mortality, significant changes in V-ATPase A expression were not detected. These results suggest that clathrin-dependent endocytosis is a biological mechanism that plays an important role during RNAi response in WCR adults. The fact that no definitive support for the roles of silA or silC in RNAi response was obtained support the idea that RNAi response varies greatly in different insect species, demanding additional studies focused on elucidating their involvement in this mechanism. |
Palavras-Chave: |
Clatrina; Endocitose. |
Thesagro: |
Gene; Inseto; Proteína. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186961/1/Clathrin-dependent.pdf
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Marc: |
LEADER 02340naa a2200289 a 4500 001 2099972 005 2019-02-18 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0201849$2DOI 100 1 $aPINHEIRO, D. H. 245 $aClathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeCont.$h[electronic resource] 260 $c2018 520 $aThe cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H+ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led to a significant decrease in the effects of laccase2 dsRNA reporter, indicating that these genes are involved in RNAi response. However, the knockdown of either Arf72A or Rab7 did not suppress the response to laccase2 dsRNA. The silencing of the silC gene did not lead to a significant reduction in mortality or increase in the expression of V-ATPase A reporter. While the silencing of the silA gene significantly decreased insect mortality, significant changes in V-ATPase A expression were not detected. These results suggest that clathrin-dependent endocytosis is a biological mechanism that plays an important role during RNAi response in WCR adults. The fact that no definitive support for the roles of silA or silC in RNAi response was obtained support the idea that RNAi response varies greatly in different insect species, demanding additional studies focused on elucidating their involvement in this mechanism. 650 $aGene 650 $aInseto 650 $aProteína 653 $aClatrina 653 $aEndocitose 700 1 $aVÉLEZ, A. M. 700 1 $aFISHILEVICH, E. 700 1 $aWANG, H. 700 1 $aCARNEIRO, N. P. 700 1 $aVALENCIA-JIMÉNEZ, A. 700 1 $aVALICENTE, F. H. 700 1 $aNARVA, E. K. 700 1 $aSIEGFRIED, B. D. 773 $tPlos One$gv. 13, n. 8, e0201849, Aug. 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
07/01/2005 |
Data da última atualização: |
07/01/2005 |
Autoria: |
ELISEI, C.; ARAÚJO, F. R.; SCOFIELD, A.; FERNANDES, K. R.; FORLANO, M. D.; SOARES, C. O.; MASSARD, C. L. |
Título: |
Análise do polimorfismo de Plasmodium juxtanucleare por meio de PCR - RFLP. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 245, 2004. Suplemento 1, ref. 156. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. |
Idioma: |
Português |
Notas: |
CNPGC. |
Palavras-Chave: |
PCR. |
Thesagro: |
Plasmodium Juxtanucleare; Polimorfismo. |
Thesaurus NAL: |
polymerase chain reaction; polymorphism. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00904naa a2200253 a 4500 001 1325691 005 2005-01-07 008 2004 bl uuuu u00u1 u #d 100 1 $aELISEI, C. 245 $aAnálise do polimorfismo de Plasmodium juxtanucleare por meio de PCR - RFLP. 260 $c2004 500 $aCNPGC. 650 $apolymerase chain reaction 650 $apolymorphism 650 $aPlasmodium Juxtanucleare 650 $aPolimorfismo 653 $aPCR 700 1 $aARAÚJO, F. R. 700 1 $aSCOFIELD, A. 700 1 $aFERNANDES, K. R. 700 1 $aFORLANO, M. D. 700 1 $aSOARES, C. O. 700 1 $aMASSARD, C. L. 773 $tRevista Brasileira de Parasitologia Veterinária, São Paulo$gv.13, p. 245, 2004. Suplemento 1, ref. 156. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004.
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