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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
15/03/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOARI, A. de J.; QUADROS, A. F. F.; KAUFFMANN, C. M.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; CORDOVIL, G. A.; GAVINHO, B. E. S.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, T. |
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Afiliação: |
ALESSANDRA DE JESUS BOARI, CPATU; AYANE F. F. QUADROS, UFRA; CATERYNNE M. KAUFFMANN, UFRA; KÉSSIA F. C. PANTOJA, Univesidade Estadual de São Paulo; RUI ALBERTO GOMES JUNIOR, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; RAIMUNDO PARENTE DE OLIVEIRA, CPATU; GABRIELE A. CORDOVIL, UFRA; BRENDA E. S. GAVINHO, UFRA; ELLIOT W. KITAJIMA, ESALQ/USP; ROSANA BLAWID, Universidade Federal Rural de Pernambuco; TATSUYA NAGATA, UNB. |
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Título: |
Near-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 46, n. 4, p. 487-491, Aug. 2021. |
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DOI: |
https://doi.org/10.1007/s40858-021-00420-w |
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Idioma: |
Inglês |
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Conteúdo: |
The sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%). MenosThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% ... Mostrar Tudo |
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Thesagro: |
Feijão; Genoma; Vigna Unguiculata; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02717naa a2200313 a 4500
001 2130679
005 2021-08-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00420-w$2DOI
100 1 $aBOARI, A. de J.
245 $aNear-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection.$h[electronic resource]
260 $c2021
520 $aThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%).
650 $aFeijão
650 $aGenoma
650 $aVigna Unguiculata
650 $aVírus
700 1 $aQUADROS, A. F. F.
700 1 $aKAUFFMANN, C. M.
700 1 $aPANTOJA, K. F. C.
700 1 $aGOMES JUNIOR, R. A.
700 1 $aFREIRE FILHO, F. R.
700 1 $aOLIVEIRA, R. P. de
700 1 $aCORDOVIL, G. A.
700 1 $aGAVINHO, B. E. S.
700 1 $aKITAJIMA, E. W.
700 1 $aBLAWID, R.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 46, n. 4, p. 487-491, Aug. 2021.
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Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
01/02/2018 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
VILELA, E. F.; RECH FILHO, E. L.; MARTHA JUNIOR, G. B.; ALVES, E. R. de A.; LOPES, M. A.; GUIMARAES, E. P.; CABRAL, P. R.; SOARES, C. O.; ROSINHA, G. M. S.; BUAINAIN, A. M.; NUTTI, M. R.; CALEGAR, G. M. |
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Afiliação: |
Evaldo Ferreira Vilela, UFV; ELIBIO LEOPOLDO RECH FILHO, Cenargen; GERALDO BUENO MARTHA JUNIOR, CNPTIA; ELISEU ROBERTO DE ANDRADE ALVES, DE/PR; MAURICIO ANTONIO LOPES, CNPAE; ELCIO PERPETUO GUIMARAES, CNPAF; Paulo Renato Cabral; CLEBER OLIVEIRA SOARES, DEIT; GRACIA MARIA SOARES ROSINHA, CNPGC; Antônio Marcio Buainain; MARILIA REGINI NUTTI, CTAA; GERALDO MAGELA CALEGAR, CNPGL. |
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Título: |
Food and Nutrition Security in Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: IANAS. Challenges and opportunities for food and nutrition security in the Americas: The view of the Academies of Sciences. Ciudad de Mexico: IANAS, 2017. p. 76-109. |
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ISBN: |
978-607-8379-29-3 |
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Idioma: |
Inglês |
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Notas: |
IANAS Regional Report november 2017. Tít. em espanhol: Food and Nutrition Security in Brazil. In: Retos y oportunidades de la seguridad alimentaria y nutricional en las Américas: El punto de vista de las Academias de Ciencias. |
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Conteúdo: |
In the past 40 years, the agricultural public and private sectors of Brazil have been working in close collaboration, to promote one of the most impressive and successful sustainable agricultural developments in a middle income country. Brazil has become an example of a food secure country and one the of world?s most important agricultural export countries. Mention should be made of the outstanding role played by the agricultural research technology developed by Brazilian research organizations, led by the agricultural research system encompassing agricultural universities, the Brazilian Agricultural Research Organization (EMBRAPA) and the state agricultural research organizations. This comprehensive executive summary outlines the future challenges and opportunities for the Brazilian agricultural sector in terms of science, technology and innovation, to keep agriculture improving its performance in a world that faces the enormous challenge of feeding a hungry population now and in the following decades. These challenges and opportunities were identified by a select group of highly qualified Brazilian researchers who have spent a lifetime generating and adapting new technology for the development of the Brazilian agriculture sector. |
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Thesagro: |
Nutrição Humana; Segurança Alimentar. |
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Thesaurus NAL: |
Food and Human Nutrition; Food security. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 02481naa a2200325 a 4500
001 2086913
005 2020-03-05
008 2017 bl uuuu u00u1 u #d
020 $a978-607-8379-29-3
100 1 $aVILELA, E. F.
245 $aFood and Nutrition Security in Brazil.$h[electronic resource]
260 $c2017
500 $aIANAS Regional Report november 2017. Tít. em espanhol: Food and Nutrition Security in Brazil. In: Retos y oportunidades de la seguridad alimentaria y nutricional en las Américas: El punto de vista de las Academias de Ciencias.
520 $aIn the past 40 years, the agricultural public and private sectors of Brazil have been working in close collaboration, to promote one of the most impressive and successful sustainable agricultural developments in a middle income country. Brazil has become an example of a food secure country and one the of world?s most important agricultural export countries. Mention should be made of the outstanding role played by the agricultural research technology developed by Brazilian research organizations, led by the agricultural research system encompassing agricultural universities, the Brazilian Agricultural Research Organization (EMBRAPA) and the state agricultural research organizations. This comprehensive executive summary outlines the future challenges and opportunities for the Brazilian agricultural sector in terms of science, technology and innovation, to keep agriculture improving its performance in a world that faces the enormous challenge of feeding a hungry population now and in the following decades. These challenges and opportunities were identified by a select group of highly qualified Brazilian researchers who have spent a lifetime generating and adapting new technology for the development of the Brazilian agriculture sector.
650 $aFood and Human Nutrition
650 $aFood security
650 $aNutrição Humana
650 $aSegurança Alimentar
700 1 $aRECH FILHO, E. L.
700 1 $aMARTHA JUNIOR, G. B.
700 1 $aALVES, E. R. de A.
700 1 $aLOPES, M. A.
700 1 $aGUIMARAES, E. P.
700 1 $aCABRAL, P. R.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
700 1 $aBUAINAIN, A. M.
700 1 $aNUTTI, M. R.
700 1 $aCALEGAR, G. M.
773 $tIn: IANAS. Challenges and opportunities for food and nutrition security in the Americas: The view of the Academies of Sciences. Ciudad de Mexico: IANAS, 2017. p. 76-109.
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