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6. | | PEIXOTO, R. C. L.; FERREIRA, J. DE. P.; SOARES, C. DE. P.; VIDAL, M. S.; BALDANI, J. I. Geração de mutante exD2 para avaliar a importância desse gene no transporte de ferro em Gluconacetobacter diazotrophicus. SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. 47 p. Biblioteca(s): Embrapa Agrobiologia. |
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8. | | SOARES, C. DE P.; FERREIRA, J. DE. P.; PEIXOTO, R. C. L.; VIDAL, M. S.; BALDANI, J. I. Envolvimento do sistema TonB-ExbB-ExbD1-ExbD2 no transporte de ferro em Gluconacetobacter diazotrophicus SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. 44 p. Biblioteca(s): Embrapa Agrobiologia. |
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9. | | SILVA, P. R. A. da; DRESCHEL, M. M.; SOARES, C. de P.; VIDAL, M. S.; BALDANI, J. I. Efeito antagônico de bactérias diazotróficas contra fungos patógenos de cana-de-açúcar In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 13., 2013, Seropédica Ciência, saúde e esporte: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2013. Biblioteca(s): Embrapa Agrobiologia. |
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10. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAÚJO, J. L. S.; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter dizaotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de pequisa e desenvolvimento, 52). Biblioteca(s): Embrapa Unidades Centrais. |
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11. | | SOARES, C. de P.; TERRA, L. A.; TADRA-SFEIR, M. Z.; VIDAL, M. S.; BALDANI, J. I. Transcriptome analysis of Gluconacetobacter diazotrophicus PAL5 strain in response to iron. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 50 Session 4, Poster IV.1 Biblioteca(s): Embrapa Agrobiologia. |
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12. | | RODRIGUES, E. P.; SOARES, C. de P.; OLIVEIRA, A. L. M. de; SIMÕES-ARAÚJO, J. L.; VIDAL, M. S.; BALDANI, J. I. Knockout of a L-amino acid oxidase flavin gene of Gluconacetobacter diazotrophicus affected the production of auxin. In: INTERNATIONAL SYMPOSIUM ON NITROGEN FIXATION WITH NON-LEGUMES, 11th., 2008, Gent, Belgium. Abstracts... Belgium: Universiteit Gent, 2008. p. 7. Resumo PS1-3. Parceria: UFRRJ; Universidade Estadual de Londrina. Biblioteca(s): Embrapa Agrobiologia. |
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13. | | RODRIGUES, E. P.; SOARES, C. de P.; OLIVEIRA, A. L. M.; VIDAL, M. S.; SIMÕES ARAÚJO, J. L.; BALDANI, J. I. Isolamento e caracterização de mutantes de Gluconacetobacter diazotrophicus deficientes na produção de auxinas. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. {São Paulo}: SBM, 2007. 1 p. 1 CD-ROM. Parceria: UFRJ, UFRRJ, UEL. Biblioteca(s): Embrapa Agrobiologia. |
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14. | | SOARES, C. DE P.; FERREIRA, J. DE P.; FRANÇA, P. V. L.; RODRIGUES, E. P.; BALDANI, J. I.; VIDAL, M. S. Iron limiting conditions affect nitrogenase activity, biofilm formation and siderophore production in gluconacetobacter diazotrophicus pal 5 strain. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçú: SBG, 2012. 67 Biblioteca(s): Embrapa Agrobiologia. |
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17. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAUJO, J. L. S. de; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter diazotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de Pesquisa e Desenvolvimento, 52). Biblioteca(s): Embrapa Agrobiologia. |
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19. | | POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. Biblioteca(s): Embrapa Agrobiologia. |
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20. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. Epub, 17 Aug., 2016. Biblioteca(s): Embrapa Agrobiologia. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
27/12/2017 |
Data da última atualização: |
03/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
ISSN: |
1572-9699 |
DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
Idioma: |
Inglês |
Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
Thesaurus NAL: |
nitrogen fixation. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02682naa a2200265 a 4500 001 2083708 005 2018-01-03 008 2017 bl uuuu u00u1 u #d 022 $a1572-9699 024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI 100 1 $aPOLESE, V. 245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource] 260 $c2017 520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. 650 $anitrogen fixation 653 $aInternal normalizing 653 $aOrganic acids 653 $aRelative expression 653 $aSugar transport 700 1 $aSOARES, C. de P. 700 1 $aSILVA, P. R. A. da 700 1 $aARAUJO, J. L. S. de 700 1 $aBALDANI, J. I. 700 1 $aVIDAL, M. S. 773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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