Data/hora: 23/06/2024 20:26:53

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 30/04/1997
Data da última atualização: | 19/10/2007
Autoria: | ARAGÃO, F. J. L.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; RIBEIRO, S. G.; SMITH, F. D.; SANFORD, J. C.; FARIA, J. C.; RECH, E. L.
Afiliação: | EMBRAPA-CENARGEN.
Título: | Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.
Ano de publicação: | 1996
Fonte/Imprenta: | Theoretical and Applied Genetics, Berlin, v.93, n.l/2, p.142-150, l996.
Páginas: | p. 142-150
Idioma: | Inglês
Conteúdo: | Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
Palavras-Chave: | Bean; Biolistica; Electrical; Feijão transgênico; Particle; Transgenic bean.
Thesagro: | Feijão; Phaseolus Vulgaris.
Thesaurus Nal: | biolistics.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

Exemplares:

CENARGEN - (10038-1) - UPC - AP - -- - 575.105 -  -  - DIS
CNPAF - (21271-1) - UPC - AP - PP - 1996.009 -  - 1996.009 - DIS