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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/02/2008 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
EGITO, A. S. do; GIRARDET, J. -M.; LAGUNA, L. E.; POIRSON, C.; MOLLÉ, D.; MICLO, L.; HUMBERT, G.; GAILLARD, J. -L. |
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Afiliação: |
ANTONIO SILVIO DO EGITO, CNPC; J.-M. Girardet, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); LUIS EDUARDO LAGUNA, CNPC; C. Poirson, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); D. Mollé, Institut National de la Recherche Agronomique; L. Miclo, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); G. Humbert, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); J.-L. Gaillard, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA). |
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Título: |
Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
International Dairy Journal, v. 17, n. 7, p. 816-825, Jul. 2007. |
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DOI: |
10.1016/j.idairyj.2006.09.012 |
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Idioma: |
Inglês |
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Conteúdo: |
Milk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target. |
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Palavras-Chave: |
Bovine casein; Cheese; Enzima coagulante; Plant rennet; Sunflower. |
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Thesagro: |
Albizia lebbeck; Caseína; Coagulação; Coalho; Extrato Vegetal; Leite. |
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Thesaurus Nal: |
Albizia; Aspartic proteinases; Chymosin; Cynara cardunculus; Kappa-casein; Proteolysis; Sheep. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02194naa a2200433 a 4500
001 1514197
005 2019-04-05
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1016/j.idairyj.2006.09.012$2DOI
100 1 $aEGITO, A. S. do
245 $aMilk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein.$h[electronic resource]
260 $c2007
520 $aMilk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target.
650 $aAlbizia
650 $aAspartic proteinases
650 $aChymosin
650 $aCynara cardunculus
650 $aKappa-casein
650 $aProteolysis
650 $aSheep
650 $aAlbizia lebbeck
650 $aCaseína
650 $aCoagulação
650 $aCoalho
650 $aExtrato Vegetal
650 $aLeite
653 $aBovine casein
653 $aCheese
653 $aEnzima coagulante
653 $aPlant rennet
653 $aSunflower
700 1 $aGIRARDET, J. -M.
700 1 $aLAGUNA, L. E.
700 1 $aPOIRSON, C.
700 1 $aMOLLÉ, D.
700 1 $aMICLO, L.
700 1 $aHUMBERT, G.
700 1 $aGAILLARD, J. -L.
773 $tInternational Dairy Journal$gv. 17, n. 7, p. 816-825, Jul. 2007.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
17/12/2021 |
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Data da última atualização: |
17/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, D. T. da; SMANIOTTO, F. A.; COSTA, I. F.; BARANZELLI, J.; MULLER, A.; SOMACAL, S.; MONTEIRO, C. S.; VIZZOTTO, M.; RODRIGUES, E.; BARCIA, M. T.; EMANUELLI, T. |
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Afiliação: |
DARIANE TRIVISIOL DA SILVA; FRANCIELE ALINE SMANIOTTO; ISABELLA FERREIRA COSTA; JULIA BARANZELLI; ALINE MULLER; SABRINA SOMACAL; CAMILA SANT'ANNA MONTEIRO; MARCIA VIZZOTTO, CPACT; ELISEU RODRIGUES; MILENE TEIXEIRA BARCIA; TATIANA EMANUELLI. |
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Título: |
Natural deep eutectic solvent (NADES): A strategy to improve the bioavailability of blueberry phenolic compounds in a ready-to-use extract. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Food Chemistry, v. 364, 130370, Dec. 2021. |
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DOI: |
doi.org/10.1016/j.foodchem.2021.130370 |
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Idioma: |
Inglês |
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Conteúdo: |
This study investigated whether a ready-to-use extract obtained using a natural deep eutectic solvent (NADES) affects the pharmacokinetic profile of blueberry phenolic compounds compared to organic solvent (SORG)-extracted compounds. SORG extract was administered as an aqueous solution after solvent removal. Wistar rats received a single dose of crude extract of blueberry obtained using NADES (CE-NADES) or SORG (CE-SORG), followed by LC-DAD-MS/MS analysis of blood and cecal feces. Non-compartmental pharmacokinetic analysis revealed that CE-NADES increased the bioavailability of anthocyanins by 140% compared to CE-SORG. CE-NADES increased the stability of phenolic compounds during in vitro digestion by delaying gastric chyme neutralization. These results suggest that besides being an eco-friendly solvent for the extraction of phytochemicals, choline chloride:glycerol:citric acid-based NADES can be used as a ready-to-use vehicle for increasing oral absorption of bioactive compounds such as anthocyanins. |
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Palavras-Chave: |
Composto. |
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Thesagro: |
Extrato; Mirtilo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229347/1/Artigo-Natural-deep-eutectic-solvent.pdf
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Marc: |
LEADER 01878naa a2200289 a 4500
001 2138030
005 2021-12-17
008 2021 bl uuuu u00u1 u #d
024 7 $adoi.org/10.1016/j.foodchem.2021.130370$2DOI
100 1 $aSILVA, D. T. da
245 $aNatural deep eutectic solvent (NADES)$bA strategy to improve the bioavailability of blueberry phenolic compounds in a ready-to-use extract.$h[electronic resource]
260 $c2021
520 $aThis study investigated whether a ready-to-use extract obtained using a natural deep eutectic solvent (NADES) affects the pharmacokinetic profile of blueberry phenolic compounds compared to organic solvent (SORG)-extracted compounds. SORG extract was administered as an aqueous solution after solvent removal. Wistar rats received a single dose of crude extract of blueberry obtained using NADES (CE-NADES) or SORG (CE-SORG), followed by LC-DAD-MS/MS analysis of blood and cecal feces. Non-compartmental pharmacokinetic analysis revealed that CE-NADES increased the bioavailability of anthocyanins by 140% compared to CE-SORG. CE-NADES increased the stability of phenolic compounds during in vitro digestion by delaying gastric chyme neutralization. These results suggest that besides being an eco-friendly solvent for the extraction of phytochemicals, choline chloride:glycerol:citric acid-based NADES can be used as a ready-to-use vehicle for increasing oral absorption of bioactive compounds such as anthocyanins.
650 $aExtrato
650 $aMirtilo
653 $aComposto
700 1 $aSMANIOTTO, F. A.
700 1 $aCOSTA, I. F.
700 1 $aBARANZELLI, J.
700 1 $aMULLER, A.
700 1 $aSOMACAL, S.
700 1 $aMONTEIRO, C. S.
700 1 $aVIZZOTTO, M.
700 1 $aRODRIGUES, E.
700 1 $aBARCIA, M. T.
700 1 $aEMANUELLI, T.
773 $tFood Chemistry$gv. 364, 130370, Dec. 2021.
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Embrapa Clima Temperado (CPACT) |
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