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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
31/10/2016 |
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Data da última atualização: |
09/02/2017 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
JARDINE, J. G.; NESHICH, I. A. P.; MAZONI, I.; YANO, I. H.; MORAES, F. R. de; SALIM, J. A; BORRO, L.; NISHIMURA, L. S.; NESHICH, G. |
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Afiliação: |
JOSÉ GILBERTO JARDINE, CNPTIA; IZABELLA AGOSTINHO PENA NESHICH, Unicamp; IVAN MAZONI, CNPTIA; INÁCIO HENRIQUE YANO, CNPTIA; FÁBIO ROGÉRIO DE MORAES, Unesp; JOSÉ AUGUSTO SALIM, FEEC/Unicamp; LUIZ BORRO, Unicamp; LETÍCIA SAYURI NISHIMURA, Unicamp; GORAN NESHICH, CNPTIA. |
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Título: |
Computational Molecular Biology and its applications in agriculture. |
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Título original: |
Biologia computacional molecular e suas aplicações na agricultura. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S. (Ed.). Information and communication technologies and their relations with agriculture. Brasília, DF: Embrapa, 2016. ch. 6, |
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Páginas: |
p. 103-118. |
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ISBN: |
978-85-7035-582-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Computational Biology, a new applied science. Rational design of drugs, pharmaceuticals and agrochemicals. Computational Biology at Embrapa. |
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Palavras-Chave: |
Bioinformática; Biologia computacional. |
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Thesagro: |
Agricultura. |
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Thesaurus Nal: |
Agriculture; Bioinformatics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01184naa a2200313 a 4500
001 2055668
005 2017-02-09
008 2016 bl uuuu u00u1 u #d
020 $a978-85-7035-582-9
100 1 $aJARDINE, J. G.
240 $aBiologia computacional molecular e suas aplicações na agricultura.
245 $aComputational Molecular Biology and its applications in agriculture.
260 $c2016
300 $ap. 103-118.
520 $aComputational Biology, a new applied science. Rational design of drugs, pharmaceuticals and agrochemicals. Computational Biology at Embrapa.
650 $aAgriculture
650 $aBioinformatics
650 $aAgricultura
653 $aBioinformática
653 $aBiologia computacional
700 1 $aNESHICH, I. A. P.
700 1 $aMAZONI, I.
700 1 $aYANO, I. H.
700 1 $aMORAES, F. R. de
700 1 $aSALIM, J. A
700 1 $aBORRO, L.
700 1 $aNISHIMURA, L. S.
700 1 $aNESHICH, G.
773 $tIn: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S. (Ed.). Information and communication technologies and their relations with agriculture. Brasília, DF: Embrapa, 2016. ch. 6
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C. |
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Afiliação: |
C. LESSARD, Agriculture and Agri-Food Canada, Western College of Veterinary Medicine; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; O. D'AMOURS, Université Laval, Canada; R. SULLIVAN, Université Laval, Canada; P. LECLERC, Université Laval, Canada; C. PALMER, University of Saskatchewan, Canada. |
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Título: |
Infertility in a beef bull due to a failure in the capacitation process. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 76, n. 5, p. 891-899, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2011.04.020 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process. MenosThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to ... Mostrar Tudo |
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Palavras-Chave: |
Absence of fertilization; Bovine infertility; Capacitation; Sperm morphology. |
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Thesaurus NAL: |
sperm motility. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02419naa a2200253 a 4500
001 1908898
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2011.04.020$2DOI
100 1 $aLESSARD, C.
245 $aInfertility in a beef bull due to a failure in the capacitation process.$h[electronic resource]
260 $c2011
520 $aThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process.
650 $asperm motility
653 $aAbsence of fertilization
653 $aBovine infertility
653 $aCapacitation
653 $aSperm morphology
700 1 $aSIQUEIRA, L. G. B.
700 1 $aD'AMOURS, O.
700 1 $aSULLIVAN, R.
700 1 $aLECLERC, P.
700 1 $aPALMER, C.
773 $tTheriogenology$gv. 76, n. 5, p. 891-899, 2011.
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