Data/hora: 09/06/2024 14:31:04

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 07/12/2011
Data da última atualização: | 05/02/2024
Autoria: | LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C.
Afiliação: | C. LESSARD, Agriculture and Agri-Food Canada, Western College of Veterinary Medicine; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; O. D'AMOURS, Université Laval, Canada; R. SULLIVAN, Université Laval, Canada; P. LECLERC, Université Laval, Canada; C. PALMER, University of Saskatchewan, Canada.
Título: | Infertility in a beef bull due to a failure in the capacitation process.
Ano de publicação: | 2011
Fonte/Imprenta: | Theriogenology, v. 76, n. 5, p. 891-899, 2011.
DOI: | https://doi.org/10.1016/j.theriogenology.2011.04.020
Idioma: | Inglês
Conteúdo: | The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process.
Palavras-Chave: | Absence of fertilization; Bovine infertility; Capacitation; Sperm morphology.
Thesaurus Nal: | sperm motility.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (18574-1) - UPC - AP - DD -  -  -  - DIS