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| Registros recuperados : 177 | |
| 24. |  | CUNHA, E. R.; SILVA, C. G.; SIQUEIRA, L. G. B.; MARTINS, C. F. Isolation, culture and cryopreservation of bovine amniotic cells with Dimethyl sulfoxide, Dimethylformamide or Glycerol. Animal Reproduction, v. 7, n. 3, p. 317, Jul./Sept. 2010. Edição dos resumos do III International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.| Biblioteca(s): Embrapa Cerrados. |
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| 28. | | FERES, L. F.; PALHAO, M. P.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Pregnancy losses after transfer of bovine embryos produced by assisted reproductive technologies. Reproduction, Fertility and Development, v. 31, n. 1, p. 167, 2019. Edição dos resumos da Annual Conference of the International Embryo Technology Society, New Orleans, Louisiana, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 29. | | NUNES, V. R. R.; SIQUEIRA, L. G. B.; SILVA, G. M. da; PFEIFER, L. F. M. Relação entre a vascularização de folículos pré-ovulatórios e o momento da ovulação de vacas Girolando. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 10.; ENCONTRO DE PÓS-GRADUAÇÃO DA EMBRAPA RONDÔNIA, 5., 2019, Porto Velho, RO. Anais... Porto Velho, RO: Embrapa Rondônia, 2019. p. 11.| Biblioteca(s): Embrapa Rondônia. |
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| 30. | | PFEIFER, L. F. M.; VIANA, J. H. M.; SIQUEIRA, L. G. B.; ARASHIRO, E. K. N. Tecnologias emergentes na ultrassonografia do trato reprodutivo. NOGUEIRA, E.; SILVA, J. C. B.; NICACIO, A. C.; MINGOTI, G. Z. (ed.). Ultrassonografia na reprodução e avaliação de carcaças em bovinos. Brasília, DF: Embrapa, 2021. Cap. 5. e-pub.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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| 37. | | CASTRO, N. A. de; SIQUEIRA, L. G. B.; VIANA, J. H. M.; PFEIFER, L. F. M. Uso da PGF como indutor de ovulação em programas de IATF em vacas de leite pós-parto. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 177 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C. |
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Afiliação: |
C. LESSARD, Agriculture and Agri-Food Canada, Western College of Veterinary Medicine; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; O. D'AMOURS, Université Laval, Canada; R. SULLIVAN, Université Laval, Canada; P. LECLERC, Université Laval, Canada; C. PALMER, University of Saskatchewan, Canada. |
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Título: |
Infertility in a beef bull due to a failure in the capacitation process. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 76, n. 5, p. 891-899, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2011.04.020 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process. MenosThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to ... Mostrar Tudo |
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Palavras-Chave: |
Absence of fertilization; Bovine infertility; Capacitation; Sperm morphology. |
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Thesaurus NAL: |
sperm motility. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02419naa a2200253 a 4500
001 1908898
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2011.04.020$2DOI
100 1 $aLESSARD, C.
245 $aInfertility in a beef bull due to a failure in the capacitation process.$h[electronic resource]
260 $c2011
520 $aThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process.
650 $asperm motility
653 $aAbsence of fertilization
653 $aBovine infertility
653 $aCapacitation
653 $aSperm morphology
700 1 $aSIQUEIRA, L. G. B.
700 1 $aD'AMOURS, O.
700 1 $aSULLIVAN, R.
700 1 $aLECLERC, P.
700 1 $aPALMER, C.
773 $tTheriogenology$gv. 76, n. 5, p. 891-899, 2011.
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