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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/06/1997 |
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Data da última atualização: |
12/06/1997 |
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Autoria: |
BUTT, T. M.; HUMBER, R. A. |
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Afiliação: |
USDA-ARS, Plant Protection Research Unit, US Plant Soil and Nutrition Laboratory, Tower Road, Ithaca, New York. |
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Título: |
An immunofluorescence study of mitosis in a mite-pathogen, Neozygites sp. (Zygomycetes: Entomophthorales). |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Protoplasma, v. 151, p. 115-123, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Mitosis in a mite-pathogenic species of Neozygites (Zygomycetes: Entomophthorales) was investigated by indirect immunofluorescence microscopy using an antibody against a-tubulin for visualization of microtubules (MTs). DAPI and rhodamine-conjugated phalloidin were used to stain chromatin and actin, respctively. Salient features of mitosis in Neozygites sp. are a strong tendency for mitotic synchrony in any given cell, conical protrucions at the poles of metaphase and anaphase and nuclei revealed by actin stain ing, absence of astral and other cytoplasmic MTs, a spindle that occupies most of the nuclear volume at metaphase, a spindle that remains symmetrical throughout most of mitosis, kinetochore MTs that shorten duriing anaphase A, a central spindle that elongates during anaphase B, pushing the daughter nuclei into the cell apices, and interpolar MTs that continue to elongate even after separation of the daughter nuclei. |
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Palavras-Chave: |
Immunofluorescence; Neozigites sp. |
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Thesaurus Nal: |
Entomophthorales; microtubules; mitosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01482naa a2200193 a 4500
001 1647922
005 1997-06-12
008 1989 bl --- 0-- u #d
100 1 $aBUTT, T. M.
245 $aAn immunofluorescence study of mitosis in a mite-pathogen, Neozygites sp. (Zygomycetes$bEntomophthorales).
260 $c1989
520 $aMitosis in a mite-pathogenic species of Neozygites (Zygomycetes: Entomophthorales) was investigated by indirect immunofluorescence microscopy using an antibody against a-tubulin for visualization of microtubules (MTs). DAPI and rhodamine-conjugated phalloidin were used to stain chromatin and actin, respctively. Salient features of mitosis in Neozygites sp. are a strong tendency for mitotic synchrony in any given cell, conical protrucions at the poles of metaphase and anaphase and nuclei revealed by actin stain ing, absence of astral and other cytoplasmic MTs, a spindle that occupies most of the nuclear volume at metaphase, a spindle that remains symmetrical throughout most of mitosis, kinetochore MTs that shorten duriing anaphase A, a central spindle that elongates during anaphase B, pushing the daughter nuclei into the cell apices, and interpolar MTs that continue to elongate even after separation of the daughter nuclei.
650 $aEntomophthorales
650 $amicrotubules
650 $amitosis
653 $aImmunofluorescence
653 $aNeozigites sp
700 1 $aHUMBER, R. A.
773 $tProtoplasma$gv. 151, p. 115-123, 1989.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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