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| Registros recuperados : 177 | |
| 121. |  | SIQUEIRA, L. G. B.; ARASHIRO, E. K.; GHETTI, A. M.; SOUZA, E. D.; FERES, L. F.; PFEIFER, L. F. M.; FONSECA, J. F. da; VIANA, J. H. M. Vascular and morphological features of the corpus luteum 12 to 20 days after timed artificial insemination in dairy cattle. Journal of Dairy Science, v. 102, n. 6, p. 5612-5626, 2019.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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| 122. | | ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. Reproductive Biology and Endocrinology, v. 11, article 73, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 123. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; DINIZ, E. S.; CAMARGO, L. S. A.; AMORIM, L. S.; FONSECA, J. F.; FERNANDES, C. A. C.; TORRES, C. A. A. Aferição de ecogenicidade luteal com o uso de diferentes transdutores de ultra-som. Acta Scientiae Veterinariae, v. 34, supl. 1, p. s281, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 124. | | CAMARGO, L. S. de A.; PEREIRA, M. M.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Altered gene expression in bovine somatic cell nuclear-transfered embryos after trichostatin a treatment. Reproduction, Fertility and Development, v. 24, n. 1, p. 121-122, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 125. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; TORRES, C. A. A.; SOUZA, E. D.; AMORIM, L. S.; OLIVEIRA, J. L.; CAMARGO, L. S. A.; Jr, P. L. J. M. Corpora lutea morphological and echotextural attributes related to plasma progesterona concentration in heifers. In: REPRODUCTION, FERTILITY AND DEVELOPMENT. 20. 2008, Anais. Colorado. Csiro, Academy of Science, 2008. 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 126. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; TORRES, C. A. A.; SOUZA, E. D.; AMORIM, L. S.; FONSECA, J. F. da; CAMARGO, L. S. A.; MONTEIRO JÚNIOR, P. L. J. Corpora lutea morphological and echotextural attributes related to plasma progesterone concentration in heifers. Reproduction, Fertility and Development, v. 20, n. 1, p. 237-238, 2008. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, Denver, Colorado, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 127. | | CARVALHEIRA, L. R.; BRANDÃO, F. Z.; GOES, T. J. F.; QUINTAO, C. C. R.; OLIVEIRA, C. S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; TORRES FILHO, R. A.; CARVALHO, B. C. de. DNA fragmentation in oocytes of Holstein x Gyr cows supplemented with organic chromium under heat stress in climatic chamber. Animal Reproduction, v. 15, n. 3, p. 418, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 128. | | VIANA, J. H. M.; ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; GHETTI, A. M.; AREAS, V. S.; GUIMARÃES, C. R. B.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C. Doppler ultrasonography as a tool for ovarian management. Animal Reproduction, v. 10, n. 3, p. 215-222, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 129. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. Theriogenology, v. 126, p. 68-74, 2019.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 130. | | ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; FONSECA, J. F. da; BRUSCHI, J. H.; CAMARGO, L. S.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Dinâmica luteal em cabras da raça Toggenburg: caracterização morfológica e endócrina. Acta Scientiae Veterinariae Porto Alegre, v. 36, Supl. 2, p. s453, 2008. Edição dos Resumos XXII Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 131. | | SOUZA, J. F. da S. e; SOUZA, E. D. de; LEMOS, D. R. de; MUNK, M.; OLIVEIRA, C. S.; QUINTAO, C. C. R.; SARAIVA, N. Z.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A. Bovine oocyte and cumulus cells can exhibit distinct response to heat shock during in vitro maturation. Animal Reproduction, v. 20, n. 2, 2023. Abstracts of 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Biblioteca(s): Embrapa Gado de Leite. |
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| 132. | | SIQUEIRA, L. G. B.; TRIBULO, P.; CHEN, Z.; DENICOL, A. C.; ORTEGA, M. S.; NEGRON-PEREZ, V.M.; KANNAMPUZHA-FRANCIS, J. P.; POHLER, K. G.; RIVERA, R. M.; HANSEN, P. J. Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. Biology of Reproduction, v. 96, n. 4, p. 743-757, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 133. | | MINARÉ, T. P.; ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CORDEIRO, C. E. C.; FERNANDES, C. A. C.; VIANA, J. H. M. Characterization of individual differences in the efficiency of superovulation in the Gyr breed. Animal Reproduction, v.10, n.3, p. 546, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 134. | | AMORIM, L. S.; TORRES, C. A. A.; AMORIM, E. A. M.; GUIMARÃES, J. D.; SILVA FILHO, J. M.; FONSECA, J. F. da; VIANA, J. H. M.; SIQUEIRA, L. G. B. Características físicas e morfológicas do sêmen de touros da raça nelore confinados e tratados com somatotrofina bovina recombinante (r-bST). Acta Scientiae Veterinariae, Porto Alegre, v. 34, supl. 1, p. s377, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 135. | | PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Biology Open, v. 8, n. 5, article bio037937, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 136. | | AMORIM, L. da S.; TORRES, C. A. A.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; GUIMARÃES, J. D.; CARVALHO, G. R. de; ALVES, N. G.; OLIVEIRA, M. M. N. F. de. Embryo development and follicular status of Toggenburg does fed urea diet. Revista Brasileira de Zootecnia, v. 40, n. 2, p. 277-285, 2011.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 137. | | AMORIM, L. S.; TORRES, C. A. A.; ALVES, N. G.; FONSECA, J. F. da; VIANA, J. H. M.; AMORIM, E. A. M.; RODRIGUES, M. T.; SIQUEIRA, L. G. B.; SOUZA, E. D.; ZAMBRINI, F.; M. JÚNIOR, P.; WALKER, D. J. Efeito do nitrogênio na dieta na qualidade de oócitos recuperados por laparotomia em cabras da raça Saanen. Acta Scientiae Veterinariae, Porto Alegre, v. 35, p. s1073, 2007. Suplemento 3. Edição dos anais da 21o. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões - SBTE, Salvador, 2007.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 138. | | AMORIM, L. S.; TORRES, C. A. A.; ALVES, N. G.; FONSECA, J. F.; VIANA, J. H. M.; AMORIM, E. A. M.; RODRIGUES, M. T.; SIQUEIRA, L. G. B.; SOUZA, E. D.; ZAMBRINI, F. N.; WALKER, D. J. Efeito do nitrogênio na dieta na qualidade de oócitos recuperados por laparotomia em cabras da raça Saanen. Acta Scientiae Veterinariae, v. 35, p. s1073, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 139. | | ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; PALHÃO, M. P.; RABELO, N. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PAIM, C. A. S.; VIANA, J. H. M. Follicular fluid and granulosa cell recovery from bovine follicles of different diameters using an adapted transvaginal-guided follicular aspiration system. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 38., 2012, Phoenix, Arizona. Abstracts... [S.l.: s.n.], 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 140. | | CARVALHEIRA, L. R.; CARVALHO, B. C. de; RIBEIRO, L. S.; GOES, T. J. F.; VIEIRA, S. F.; FONTES, W. F.; TORRES FILHO, R. A.; OLIVEIRA, C. S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; BRANDÃO, F. Z. Follicular population and oocyte quality from Holstein x Gyr cows under heat stress in climatic chamber and supplemented with organic chromium. Animal Reproduction, v. 15, n. 3, p. 403, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 177 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
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DOI: |
10.1016/j.theriogenology.2018.12.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production. |
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Thesaurus NAL: |
Embryo transfer; Luteinizing hormone. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02857naa a2200253 a 4500
001 2106166
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource]
260 $c2019
520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.
650 $aEmbryo transfer
650 $aLuteinizing hormone
653 $aIn vivo embryo production
700 1 $aARASHIRO, E. K. N.
700 1 $aMINARE, T. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aGRAZIA, J. G. V.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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