Data/hora: 23/06/2024 07:29:47

Biblioteca(s): 	Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	21/02/2017	
Data da última atualização: 	30/01/2023	
Autoria: 	WOHLRES-VIANA, S.; ARASHIRO, E. K. N; MACHADO, M. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; VIANA, J. H. M.	
Afiliação: 	Wohlres-Viana S., UFJF; Arashiro E. K. N., UNIV. FEDERAL DE ALFENAS; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; M. P. PALHÃO, UNIV. FEDERAL DE ALFENAS; JOAO HENRIQUE MOREIRA VIANA, Cenargen.	
Título: 	Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Reproduction Fertility and Development, v. 29, n. 10, p. 1958-1970, 2017.	
DOI: 	10.1071/RD16284	
Idioma: 	Inglês	
Notas: 	Publicado online em 19 nov. 2016.	
Conteúdo: 	Abstract The aim of the present study was to characterise the roles of intrafollicular oestradiol production and granulosa cell (GC) expression of the LH receptor (LHR) gene and its isoforms during follicular deviation in Bos indicus. Follicular wave emergence was synchronised in heifers from a Bos taurus dairy (Holstein; n=10) and a B. indicus dairy breed (Gir; n=10). Follicles were aspirated individually at sizes corresponding to the periods of predeviation, deviation and postdeviation. Intrafollicular oestradiol (IF-E2) and progesterone (IF-P4) concentrations were determined in the follicular fluid (FF) by radioimmunoassay, and relative expression of P450 aromatase (CYP19A1) and LHR forms was evaluated in GC using real-time quantitative-polymerase chain reaction. Despite differences in the size of the dominant follicle at deviation, changes in CYP19A1 expression and IF-E2 concentrations were similar in follicles of the same diameter in both breeds. A peak in total LHR expression occurred after follicular deviation in association with low expression of LHR isoforms. The results suggest that regulation of LHR function by sequential changes in the expression pattern of LHR isoforms may play a role in the early deviation of the dominant follicle, as observed in B. indicus breeds.	
Palavras-Chave: 	Bovine; Follicular dynamics.	
Thesagro: 	Bos Indicus.	
Thesaurus Nal: 	Alternative splicing; Steroidogenesis.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (23402-1) - UPC - AP - DD -  -  -  - DIS
CENARGEN - (37041-1) - UPC - AP - DD -  -  -  - DIS