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| Registros recuperados : 136 | |
| 101. |  | CUNHA, J. R. B.; WISCHRAL, D.; PELAEZ, R. D. R.; JESUS, M. A. de; SALES-CAMPOS, C.; CAMPANHA, R. B.; MENDES, T. D.; MENDONCA, S.; DIAS, E. S.; SIQUEIRA, F. G. de. Bioactives and extracellular enzymes obtained from fermented macrofungi cultivated in cotton and Jatropha seed cakes. Microorganisms , v. 10, n. 8, 1670, 2022.| Biblioteca(s): Embrapa Agroenergia. |
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| 102. | | MAGALHAES JUNIOR, A. M. de; FAGUNDES, P. R. R.; FRANCO, D. F.; MORAIS, O. P. de; SIQUEIRA, F. G. de; STRECK, E. A.; AGUIAR, G. A.; FACCHINELLO, P. H. K. BRS AG: first cultivar of irrigated rice used for alcohol production or animal feed. Crop Breeding and Applied Biotechnology, v. 17, p. 72-77, 2017.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Clima Temperado. |
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| 103. | | SILVA, E. A. da; PACHECO, T. F.; MENDES, T. D.; LEMOS, M.; CARVALHO, F. B. de P.; FAVARO, L. C. de L.; MENDONCA, S.; SIQUEIRA, F. G. de; SOUZA JUNIOR, M. T. Combinação de extratos enzimáticos de basidiomicetos e ascomicetos para hidrólise do cacho vazio de dendê. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 88-94.| Biblioteca(s): Embrapa Agroenergia. |
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| 104. | | SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. Analytical Methods, n. 2, 2020.| Biblioteca(s): Embrapa Agroenergia. |
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| 105. | | SANTANA, M. W. P. R. de; RODRIGUES, K. B.; SANTOS, F. P. dos; MENDES, T. D.; DAMASO, M. C. T.; FAVARO, L. C. de L.; SIQUEIRA, F. G. de. Clonagem e expressão de genes de monoxigenases de polissacarídeos líticas (LPMOs) de fungos em Pichia pastoris. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 36-47.| Biblioteca(s): Embrapa Agroenergia. |
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| 106. | | VIEIRA, V. de O.; CONCEIÇÃO, A. A.; SILVA, C. de O. G.; ROMERO-PELAEZ, R. D.; LOBO JUNIOR, M.; ABDELNUR, P. V.; ALMEIDA, J. R. M. de; ALMEIDA, E. G.; SIQUEIRA, F. G. de. Characterization of extracellular secondary metabolites in Oudemansiella canarii BRM-044600 displaying antifungal activity against the phytopathogen Sclerotinia sclerotiorum. World Journal of Microbiology and Biotechnology, v. 37, 203, 2021.| Biblioteca(s): Embrapa Agroenergia; Embrapa Arroz e Feijão. |
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| 107. | | MENEZES, B. S.; SOLIDADE, L. S.; CONCEIÇÃO, A. A.; SANTOS JUNIOR, M. N.; LEAL, P. L.; BRITO, E. S. de; CANUTO, K. M.; MENDONCA, S.; SIQUEIRA, F. G. de; MARQUES, L. M. Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-in ammatory properties. AMB Express, v. 10, n. 117, 2020.| Biblioteca(s): Embrapa Agroenergia. |
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| 108. | | MENEZES, B. S.; SOLIDADE, L. S.; CONCEIÇÃO, A. A.; SANTOS JUNIOR, M. N.; LEAL, P. L.; BRITO, E. S. de; CANUTO, K. M.; MENDONCA, S.; SIQUEIRA, F. G. de; MARQUES, L. M. Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties. AMB Express, v. 10, n. 117, 2020.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 109. | | MENEZES, B. dos S.; MORAIS, K. dos S.; LEITE, T. C. R.; ALCANTARA, L. M.; FILHO, E. X. F.; BRASIL, B. dos S. A. F.; SIQUEIRA, F. G. de. Production of holocellulases and amylases by filamentous fungi grown on alternative substrates. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 110. | | LYRA, B. M.; GUIMARÃES, M. B.; PEREIRA, L. C.; VALE, J. do; CAMPANHA, R. B.; CONCEIÇÃO, A. A.; BATISTA, P. O. M. D.; SIQUEIRA, F. G. de; MENDONCA, S. Propriedades antimicrobianas e antioxidantes de extratos produzidos a partir de colonizados de basidiomicetos em tortas oleaginosas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 29-36 il.| Biblioteca(s): Embrapa Agroenergia. |
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| 111. | | CUNHA, J. R. B.; WISCHRAL, D.; PELÁEZ, R. D. R.; MAGALHÃES, P. de O.; GUIMARÃES, M. B.; JESUS, M. A. de; SALES-CAMPOS, C.; MENDES, T. D.; DIAS, E. S.; MENDONCA, S.; SIQUEIRA, F. G. de. Aqueous extracts of fermented macrofungi cultivated in oilseed cakes as a carbon source for probiotic bacteria and potential antibacterial activity. Metabolites, v. 13, n. 854, 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 112. | | NETO, C. B. S.; GOMES, T. G.; ARAÚJO, A. P.; SOUZA, A. de; MIDORIKAWA, G. E. O.; ALVES, G. S. C.; SIQUEIRA, F. G. de; MILLER, R. N. G. Análise transcriptômica de genes de macrofungos durante a destoxificação em torta de caroço de algodão. IN: V SIMPÓSIO DO PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA MOLECULAR, 5., Brasília, DF. [Anais...] Brasília: UnB, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 113. | | CASTRO, C. P. de; ARAÚJO, A. P. F.; CUNHA, J. R. B.; SOARES NETO, C. B.; CONCEIÇÃO, A. A.; ABREU, M. L. T. de; DIAS, E. S.; MENDONCA, S.; SIQUEIRA, F. G. de. Fistulina hepatica CC102: pré-tratamento biológico da torta do caroço de algodão em dietas para suínos. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 34.| Biblioteca(s): Embrapa Agroenergia. |
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| 114. | | SANTANA, T. D. D.; MAGALHÃES, P. O. B.; SIQUEIRA, F. G. de; MENDONCA, S.; FERNANDES, A. P. A.; GODOY, T. G.; MACHADO, A. E. V.; COSTA, C. M. C.; URBEN, A. F.; HELM, C. V.; COSTA, A. M. Fitases, proteases e lacases produzidas por basidiomicetos em cultivo submerso com tortas de pinhão-manso ou caroço de algodão como fonte de carbono. In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba. Anais do simpósio. Sorocaba: UNISO; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2015. p. 216-217.. Pôster 27.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 115. | | SANTANA, T. D. D.; MAGALHÃES, P. O. B.; SIQUEIRA, F. G. de; MENDONCA, S.; FERNANDES, A. P. A.; GODOY, T. G.; MACHADO, A. E. V.; COSTA, C. M. C.; URBEN, A. F.; HELM, C. V.; COSTA, A. M. Fitases, proteases e lacases produzidas por basidiomicetos em cultivo submerso com tortas de pinhão-manso ou caroço de algodão como fonte de carbono. In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba. Anais do simpósio. Sorocaba: UNISO; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2015. p. 216-217. Pôster 27.| Biblioteca(s): Embrapa Florestas. |
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| 116. | | SANTANA, T. D. D.; MAGALHÃES, P. O. B.; SIQUEIRA, F. G. de; MENDONCA, S.; FERNANDES, A. P. A.; GODOY, T. G.; MACHADO, A. E. V.; COSTA, C. M. C.; URBEN, A. F.; HELM, C. V.; COSTA, A. M. Fitases, proteases e lacases produzidas por basidiomicetos em cultivo submerso com tortas de pinhão-manso ou caroço de algodão como fonte de carbono In: SIMPÓSIO INTERNACIONAL SOBRE COGUMELOS NO BRASIL, 8.; SIMPÓSIO NACIONAL SOBRE COGUMELOS COMESTÍVEIS, 7., 2015, Sorocaba, SP. Anais ... Sorocaba: Uniso, 2015. Pôster 27| Biblioteca(s): Embrapa Agroenergia. |
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| 117. | | RODRIGUES, D. A.; DEMUNER, A. J.; BARBOSA, L. C. A.; PEREIRA, G. A. M.; FABRIS, J. D.; SIQUEIRA, F. G. de; PEREIRA, M. T.; SILVA JUNIOR, A.; CARVALHO, O. V. Removing phorbol esters from the biomass to add extra value to the byproduct from deoiling seeds of Jatropha curcas in the biodiesel industry. Biomass Conversion and Biorefinery, 2021. On-line first.| Biblioteca(s): Embrapa Agroenergia. |
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| 118. | | SOARES NETO, C. B.; CONCEIÇÃO, A. A.; GOMES, T. G.; RIBEIRO, J. A. de A.; CAMPANHA, R. B.; BARROSO, P. A. V.; MACHADO, A. E. V.; MENDONCA, S.; SIQUEIRA, F. G. de; MILLER, R. N. G. A Comparison of Physical, Chemical, Biological and Combined Treatments for Detoxifcation of Free Gossypol in Crushed Whole Cottonseed. Waste and Biomass Valorization, v. 12, p. 3965-3975, 2021. PDF: il. color.| Biblioteca(s): Embrapa Agroenergia; Embrapa Territorial. |
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| 119. | | SOARES NETO, C. B.; CONCEIÇÃO, A. A.; GOMES, T. G.; RIBEIRO, J. A. de A.; CAMPANHA, R. B.; BARROSO, P. A. V.; MACHADO, A. E. V.; MENDONCA, S.; SIQUEIRA, F. G. de; MILLER, R. N. G. A Comparison of Physical, Chemical, Biological and Combined Treatments for Detoxification of Free Gossypol in Crushed Whole Cottonseed. Waste and Biomass Valorization, nov. 2020. On-line first. Data Availability: Sequences enabling molecular identification were deposited in GenBank (https://www.ncbi.nlm.nih.gov) under accession numbers MK603977, MK603978, MK603976, MK603979 and MK603975.| Biblioteca(s): Embrapa Agroenergia. |
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| 120. | | GOMES, T. G.; MACHADO, A. E. V.; ARAÚJO, A. P.; LAVIOLA, B. G.; RIBEIRO, J. A. de A.; CAMPANHA, R. B.; HELM, C. V.; MENDONCA, S.; SIQUEIRA, F. G. de. Degradação de ésteres de forbol da torta de pinhão-manso por macrofungos e potencial como substrato para produção de cogumelos comestíveis. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 95-97.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 136 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/03/2020 |
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Data da última atualização: |
27/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
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Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
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Palavras-Chave: |
Caracterização de LPMOs. |
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Categoria do assunto: |
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Marc: |
LEADER 02626naa a2200241 a 4500
001 2121326
005 2020-04-27
008 2020 bl uuuu u00u1 u #d
100 1 $aSILVA, C. de O. G.
245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource]
260 $c2020
520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products.
653 $aCaracterização de LPMOs
700 1 $aTEIXEIRA, S. T.
700 1 $aRODRIGUES, K. B.
700 1 $aSOUZA, A. A.
700 1 $aMONCLARO, A. V.
700 1 $aMENDES, T. D.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aFAVARO, L. C. de L.
700 1 $aABDELNUR, P. V.
773 $tAnalytical Methods$gn. 2, 2020.
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