Data/hora: 22/06/2024 02:17:19

Biblioteca(s): | Embrapa Agroenergia; Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 06/10/2021
Data da última atualização: | 10/12/2021
Autoria: | PELÁEZ, R. D. R.; WISCHRAL, D.; MENDES, T. D.; PACHECO, T. F.; URBEN, A. F.; HELM, C. V.; MENDONCA, S.; BALAN, V.; SIQUEIRA, F. G. de.
Afiliação: | RUBÉN DARÍO ROMERO PELÁEZ, UFT; DAIANA WISCHRAL; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; ARAILDE FONTES URBEN, Cenargen; CRISTIANE VIEIRA HELM, CNPF; SIMONE MENDONCA, CNPAE; VENKATESH BALAN, University of Houston, United States; FELIX GONCALVES DE SIQUEIRA, CNPAE.
Título: | Co-culturing of micro- and macro-fungi for producing highly active enzyme cocktail for producing biofuels.
Ano de publicação: | 2021
Fonte/Imprenta: | Bioresource Technology Reports, v. 16, 100833, Dec. 2021. 8 p.
DOI: | https://doi.org/10.1016/j.biteb.2021.100833
Idioma: | Inglês
Conteúdo: | Abstract: Mesocarp fiber (MF) and oil palm decanter cake (OPDC) are inexpensive substrates from oil palm refineries with potential to reduce fungal enzyme costs. This work demonstrated the producing enzymes method from mono- and co-cultures of micro- and macro-fungi cultured in MF or OPDC. Macrofungal species evaluated include Panus lecomtei, Trametes versicolor, Schizophyllum commune, Coprinus sp., Chrysosporium lucknowense, and Laetisaria arvalis. Secreted enzyme activities from individually cultured macrofungal strains were compared with Trichoderma reesei enzymes. Fungi co-culturing were evaluated in plates and submerged fermentation. Enzyme cocktail produced by co-culturing T. reesei and P. lecomtei exhibited higher laccase, peroxidases, hemicellulase and cellulase activities. Pretreated sugarcane bagasse (PSB) saccharification, with co-cultured enzyme cocktail, produced 12.3 g.L?1 of glucose, which was 44.7% higher when compared with T. reesei that produced 8.5 g.L?1. This work is the first to report co-culturing fungi to produce superior enzymes cocktail from OPDC that will benefit future lignocellulosic biorefinery.
Palavras-Chave: | Biomass saccharification; Co-cultures; Oil palm decanter cake; Panus lecomtei; T reesei.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

Registro original: | Embrapa Agroenergia (CNPAE)

Exemplares:

CENARGEN - (38634-1) - UPC - AP - DD -  -  -  - DIS
CNPF - (57849-1) - UPC - AP - DD -  -  -  - DIS
CNPAE - (3964-1) - UPC - AP - DD -  -  -  - DIS