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| Registros recuperados : 573 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/09/2002 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
SANTOS, C. A. F.; SIMON, P. W. |
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Afiliação: |
CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 25, n. 2, p. 195-201, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasília x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paried fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six conserved AFLPs only one mapped to different linkage groups in the two populations while four of the less-conserved bands mapped to different linkage groups. Of eight SCAR (sequence characterized amplified regions) primers tested, one conserved AFLP resulted in co-ddominant markers in both populations. Screening among 14 carrot inbreds or cultivars with three AFLP-SCAR primers revealed clear and polymorphic PCR products, with similar molecular sizes on agarose gels. The development of co-dominant markers based on conserved AFLP fragments will be useful to detect seed mixtures among hybrids, to improve and to merge linkage maps and to study diversity and phylogenetic relationships. |
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Palavras-Chave: |
AFLP; Carrot; Genetics markers; Melhoramento genético; Microsatélite; Microsatellites; Molecular markers; SCAR; Sequenciamento. |
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Thesagro: |
Cenoura; Daucus Carota; DNA; Marcador Molecular. |
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Thesaurus NAL: |
Carrots. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166636/1/Carlos.pdf
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Marc: |
LEADER 02287naa a2200301 a 4500
001 1150556
005 2017-11-13
008 2002 bl uuuu u00u1 u #d
100 1 $aSANTOS, C. A. F.
245 $aSome AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot.
260 $c2002
520 $aAmplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasília x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paried fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six conserved AFLPs only one mapped to different linkage groups in the two populations while four of the less-conserved bands mapped to different linkage groups. Of eight SCAR (sequence characterized amplified regions) primers tested, one conserved AFLP resulted in co-ddominant markers in both populations. Screening among 14 carrot inbreds or cultivars with three AFLP-SCAR primers revealed clear and polymorphic PCR products, with similar molecular sizes on agarose gels. The development of co-dominant markers based on conserved AFLP fragments will be useful to detect seed mixtures among hybrids, to improve and to merge linkage maps and to study diversity and phylogenetic relationships.
650 $aCarrots
650 $aCenoura
650 $aDaucus Carota
650 $aDNA
650 $aMarcador Molecular
653 $aAFLP
653 $aCarrot
653 $aGenetics markers
653 $aMelhoramento genético
653 $aMicrosatélite
653 $aMicrosatellites
653 $aMolecular markers
653 $aSCAR
653 $aSequenciamento
700 1 $aSIMON, P. W.
773 $tGenetics and Molecular Biology$gv. 25, n. 2, p. 195-201, 2002.
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Embrapa Semiárido (CPATSA) |
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