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61. | | PASSOS, S. R.; CORREIA, M. E. F.; SOARES, L. H. de B.; SIMÕES ARAÚJO, J. L.; RUMJANEK, N. G.; XAVIER, G. R. Isolamento de microrganismos com potencial celulolítico e fixadores de nitrogênio associados ao trato intestinal do gongolo (Trigoniulus corallinus). In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009. Biblioteca(s): Embrapa Agrobiologia. |
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62. | | SILVA, H. A. P. da; GONÇALO, T. P.; GALISA, P. de S.; MENESES, C. H. S. G.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L. Prospecção de genes expressos em nódulos de feijão-caupi submetido ao estresse térmico e hídrico. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2009. Biblioteca(s): Embrapa Agrobiologia. |
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63. | | ALVES, G. C.; GALISA, P. S.; MACEDO, A. V. M.; SANTOS, C. L. R.; VITAL, M. S.; SIMÕES-ARAÚJO, J. L .; REIS, V. M. Prospection of differentially expressed genes in two maize genotypes inoculated with Herbaspirillum Seropédicae. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p Biblioteca(s): Embrapa Agrobiologia. |
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64. | | ZILLI, J. É.; VALICHESKI, R. R.; RUMJANEK, N. G.; SIMÕES-ARAÚJO, J. L.; FREIRE FILHO, F. R.; NEVES, M. C. P. Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do Cerrado em caupi. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 5, p. 811-818, maio 2006. Biblioteca(s): Embrapa Meio-Norte. |
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65. | | ZILLI, J. E.; VALICHESKI, R. R.; RUMJANEK, N. G.; SIMÕES-ARAÚJO, J. L.; FREIRE FILHO, F. R.; NEVES, M. C. P. Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do cerrado em caupi. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 5, p. 811-818, mai. 2006. Symbiotic efficiency of cowpea Bradyrhizobium strains in cerrado soils. Biblioteca(s): Embrapa Agrobiologia. |
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66. | | GALVÃO, P. G.; GITAHY, P. de M.; MELO, L. H. V.; TEIXEIRA, M. S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Caracterização preliminar do plasmídeo pBt44 de Bacillus thuringiensis subsp. Kurstaki S76 com atividade contra a broca da cana-de-açúcar Diatraea saccharalis. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida. Resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 p. 1 CD-ROM. SICONBIOL. Biblioteca(s): Embrapa Agrobiologia. |
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69. | | SILVA, C. F. da; SIMÕES-ARAÚJO, J. L.; SILVA, E. M. R. da; PEREIRA, M. G.; FREITAS, M. S. M.; MARTINS, M. A.; SAGGIN JÚNIOR, O. J. Fungos micorrízicos arbusculares e proteína do solo relacionada à glomalina em área degradada por extração de argila e revegetada com eucalipto e acácia. Ciência Florestal, Santa Maria, RS, v. 22, n. 4, p. 749-761, out./dez. 2012. Biblioteca(s): Embrapa Florestas. |
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70. | | CORRÊA, R. L.; SILVA, T. F.; SIMÕES-ARAÚJO, J. L.; BARROSO, P. A. V.; VIDAL, M. S.; VASLIN, M. F. S. Molecular characterization of a virus from the family luteoviridae associated with cotton blue disease. Archives of Virology, v. 7, n. 150, p. 1357-1367, jul., 2005. Biblioteca(s): Embrapa Algodão. |
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71. | | CORRÊA, R. L.; SILVA, T. F.; SIMÕES-ARAÚJO, J. L.; BARROSO, P. A. V.; VIDAL, M. S.; VASLIN, M. F. S. Molecular characterization of a virus from the family Luteoviridae associated with cotton blue disease. Archives of Virology, Vienna, v. 150, n. 7, p. 1357-1367, jul. 2005. Biblioteca(s): Embrapa Agrobiologia. |
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72. | | RODRIGUES, E. P.; SOARES, C. P.; SILVA, J. R.; OLIVEIRA, A. L. M.; SIMÕES-ARAÚJO, J. L.; VIDAL, M. S.; BALDANI, J. I. Mutação na região regulatória do grupo gênico tonB-exBD1D2 sugere o seu envolvimento no transporte de auxinas em Gluconacetobacter diazotrophicus. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Anais... Salvador: SBG, 2008. p.120. UFRJ; UFRRJ; UEL. Biblioteca(s): Embrapa Agrobiologia. |
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74. | | MELO, L. H. V. de; GALVÃO, P. G.; GITAHY, P. de M.; DE SOUZA, M. T.; SILVA, F. R.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Sequenciamento preliminar de um plasmídeo de Bacillus thuringiensis subsp. Kurstaki s76. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. {São Paulo}: SBM, 2007. 1 p. 1 CD-ROM. Parceria: não tem Biblioteca(s): Embrapa Agrobiologia. |
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75. | | CHEN, W. -M.; FARIA, S. M. de; STRALIOTTO, R.; PITARD, R. M.; SIMÕES-ARAÚJO, J. L.; CHOU, J. -H.; CHOU, Y. -J.; BARRIOS, E.; PRESCOTT, A. R.; ELLIOTT, G. N.; SPRENT, J. I.; YOUNG, J. P. W.; JAMES, E. K. Proof that Burkholderia strains form effective symbioses with legumes> a study of novel Mimosa-nodulating strains from south America. Applied and Environmental Microbiology, Washington, v. 71, n. 11, p. 7461-7471, nov. 2005. Biblioteca(s): Embrapa Agrobiologia. |
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76. | | ROUWS, L. F. M.; MENESES, C. H. G.; GALVÃO, P. G.; VENTURA, H, G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitogen fixation process and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p Biblioteca(s): Embrapa Agrobiologia. |
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77. | | ROUWS, L. F. M.; MENESSES, C. H. G.; GALVÃO, P. G.; VENTURA, H. G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitrogen fixation processs and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p Biblioteca(s): Embrapa Agrobiologia. |
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78. | | FISCHER, D.; PFITZNER, B.; SCHIMID, M.; SIMÕES-ARAUJO J. L.; REIS, V. M.; PEREIRA, W.; ORMEÑO-ORRILHO, E.; HAI, B.; HOFMANN, A.; SCHLOTER, M.; MARTINEZ-ROMERO, E.; BALDANI, J. I.; HARTMANN, A. Molecular characterisaton of the dizotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.) Plant and Soil Online, 27 may 2011. Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 78 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
07/11/2005 |
Data da última atualização: |
07/11/2005 |
Autoria: |
CHEN, W. -M.; FARIA, S. M. de; STRALIOTTO, R.; PITARD, R. M.; SIMÕES-ARAÚJO, J. L.; CHOU, J. -H.; CHOU, Y. -J.; BARRIOS, E.; PRESCOTT, A. R.; ELLIOTT, G. N.; SPRENT, J. I.; YOUNG, J. P. W.; JAMES, E. K. |
Título: |
Proof that Burkholderia strains form effective symbioses with legumes> a study of novel Mimosa-nodulating strains from south America. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 71, n. 11, p. 7461-7471, nov. 2005. |
Idioma: |
Inglês |
Conteúdo: |
Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (Br3461) and Mimosa priga (MAP3-5), and hence this confirms strongly that Burkholderia strains can from effective symbioses with legumes. MenosTwenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (... Mostrar Tudo |
Palavras-Chave: |
Biological nitrogen fixation; BNF; Burkholderia caribensis; Burkholderia phymatum; Burkholderia tuberum; FBN; Fixação biológica de mitrogênio; Mimosa bumucronata. |
Thesagro: |
Ácido Nucléico; DNA; Simbiose. |
Thesaurus NAL: |
Mimosa pigra; Mimosa pudica; nucleic acids; symbiosis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02937naa a2200445 a 4500 001 1597242 005 2005-11-07 008 2005 bl --- 0-- u #d 100 1 $aCHEN, W. -M. 245 $aProof that Burkholderia strains form effective symbioses with legumes> a study of novel Mimosa-nodulating strains from south America. 260 $c2005 520 $aTwenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (Br3461) and Mimosa priga (MAP3-5), and hence this confirms strongly that Burkholderia strains can from effective symbioses with legumes. 650 $aMimosa pigra 650 $aMimosa pudica 650 $anucleic acids 650 $asymbiosis 650 $aÁcido Nucléico 650 $aDNA 650 $aSimbiose 653 $aBiological nitrogen fixation 653 $aBNF 653 $aBurkholderia caribensis 653 $aBurkholderia phymatum 653 $aBurkholderia tuberum 653 $aFBN 653 $aFixação biológica de mitrogênio 653 $aMimosa bumucronata 700 1 $aFARIA, S. M. de 700 1 $aSTRALIOTTO, R. 700 1 $aPITARD, R. M. 700 1 $aSIMÕES-ARAÚJO, J. L. 700 1 $aCHOU, J. -H. 700 1 $aCHOU, Y. -J. 700 1 $aBARRIOS, E. 700 1 $aPRESCOTT, A. R. 700 1 $aELLIOTT, G. N. 700 1 $aSPRENT, J. I. 700 1 $aYOUNG, J. P. W. 700 1 $aJAMES, E. K. 773 $tApplied and Environmental Microbiology, Washington$gv. 71, n. 11, p. 7461-7471, nov. 2005.
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