Data/hora: 21/06/2024 22:21:28

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	27/10/2004	
Data da última atualização: 	04/06/2018	
Autoria: 	CASTRO, C. S. de; SILVESTRE. F. G.; ARAUJO, S. C.; YASBECK, G. de M.; MANGILI, O. C.; CRUZ, I.; CHAVEZ-OLORTEGUI, C.; KALAPOTHAKIS, E.	
Afiliação: 	IVAN CRUZ, CNPMS.	
Título: 	Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Laxosceles intermedia.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	Toxicon, Elmsford, v. 44, p. 273-280, 2004.	
Idioma: 	Inglês	
Conteúdo: 	The venom of Loxosceles intermedia was investigated for the presence of insecticidal toxins active against Spodoptera frugiperda (Lepidoptera: Noctuidade), an insect that has caused great reductions in corn production in Brazil. A combination of gel filtration (Sephadex G-100) and ion-exchange chromatography (Carboxymethyl Cellulose, CM 52) resulted in four major fractions that were submitted to biological assay. Fraction 4 was further purified by a reverse phase HPLC (C18 Column) resulting in peptides active against Spodoptera frugiperda. Three new potential insecticidal toxins named LiTx×1, LiTx×2 and LiTx×3 were identified. The partial amino terminal sequences of these peptides were obtained and used to clone the corresponding cDNAs with the help of degenerate oligonucleotides. The amino acid sequence deduced from the cDNA of LiTx×1, LiTx×2 and LiTx×3 revealed mature proteins of approximately 7.4, 7.9 and 5.6 kDa.	
Palavras-Chave: 	cDNA; Insecticidal toxins; LiTx×1; LiTx×2; LiTx×3; Loxosceles intermedia; Spider; Toxin; Venom.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (17177-1) - UPC - AP - DD -  -  -  - DIS