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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
17/08/2023 |
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Autoria: |
HOOKEY, J. V.; RICHARDSON, J. F.; COOKSON, B. D. |
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Título: |
Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 36, n. 4, p. 1083-1089, Apr. 1998. |
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DOI: |
10.1128/JCM.36.4.1083-1089.1998. |
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Idioma: |
Inglês |
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Conteúdo: |
A typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates. MenosA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both stra... Mostrar Tudo |
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Palavras-Chave: |
Bacterial typing techniques; DNA bacterial; Methicillin Resistance. |
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Thesagro: |
Biologia Molecular; Microbiologia; Staphylococcus Aureus. |
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Thesaurus Nal: |
Coagulase positive staphylococci; Genotype; Polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02530naa a2200265 a 4500
001 1524190
005 2023-08-17
008 1998 bl uuuu u00u1 u #d
024 7 $a10.1128/JCM.36.4.1083-1089.1998.$2DOI
100 1 $aHOOKEY, J. V.
245 $aMolecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.$h[electronic resource]
260 $c1998
520 $aA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates.
650 $aCoagulase positive staphylococci
650 $aGenotype
650 $aPolymerase chain reaction
650 $aBiologia Molecular
650 $aMicrobiologia
650 $aStaphylococcus Aureus
653 $aBacterial typing techniques
653 $aDNA bacterial
653 $aMethicillin Resistance
700 1 $aRICHARDSON, J. F.
700 1 $aCOOKSON, B. D.
773 $tJournal of Clinical Microbiology$gv. 36, n. 4, p. 1083-1089, Apr. 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
18/04/2022 |
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Data da última atualização: |
18/04/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GASPARONI, W.; FERREIRA, A. P. L.; GENRO, T. C. M.; SILVEIRA, M. C. T. da. |
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Afiliação: |
WENDELL GASPARONI, UNIPAMPA; ANA PAULA LÜDTKE FERREIRA, UNIPAMPA; TERESA CRISTINA MORAES GENRO, CPPSUL; MARCIA CRISTINA T DA SILVEIRA, CPPSUL. |
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Título: |
H-Pasture: uma aplicação móvel para estimativa da disponibilidade de forragem e ajuste de carga em pastagens sob lotação contínua ou rotativa. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 13., 2021, Bagé. Anais... Bagé: Universidade Federal do Pampa, 2021. |
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Páginas: |
p. 154-163. |
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Idioma: |
Português |
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Notas: |
SBIAGRO 2021. |
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Conteúdo: |
Abstract. This article introduces the mobile application H-Pasture, which uses linear regression models to provide tools for helping farmers and rural technicians perform grazing management activities using the pasture height metric. H-Pasture uses Google?s Flutter/Dart technologies, makes forage weight estimations within the registered areas, builds reports, and produces recommendations for stocking rate under rotative or variable rate continuous pasture. Resumo. Este trabalho apresenta o aplicativo móvel H-Pasture, que usa modelos de regressão linear para fornecer ferramentas que auxiliem produtores e técnicos rurais no manejo de pastagem por meio da métrica de altura do pasto. O aplicativo foi desenvolvido com a tecnologia Flutter/Dart e faz estimativas de massa de forragem nas áreas cadastradas, gera relatórios e produz recomendações para auxiliar no ajuste de carga em pastagens sob pastejo rotativo ou contínuo com taxa variável. |
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Palavras-Chave: |
Aplicativo móvel. |
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Thesagro: |
Forragem; Pastagem; Taxa de Lotação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142268/1/sbiagro2021-H-Pasture.pdf
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Marc: |
LEADER 01727nam a2200217 a 4500
001 2142268
005 2022-04-18
008 2021 bl uuuu u00u1 u #d
100 1 $aGASPARONI, W.
245 $aH-Pasture$buma aplicação móvel para estimativa da disponibilidade de forragem e ajuste de carga em pastagens sob lotação contínua ou rotativa.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 13., 2021, Bagé. Anais... Bagé: Universidade Federal do Pampa$c2021
300 $ap. 154-163.
500 $aSBIAGRO 2021.
520 $aAbstract. This article introduces the mobile application H-Pasture, which uses linear regression models to provide tools for helping farmers and rural technicians perform grazing management activities using the pasture height metric. H-Pasture uses Google?s Flutter/Dart technologies, makes forage weight estimations within the registered areas, builds reports, and produces recommendations for stocking rate under rotative or variable rate continuous pasture. Resumo. Este trabalho apresenta o aplicativo móvel H-Pasture, que usa modelos de regressão linear para fornecer ferramentas que auxiliem produtores e técnicos rurais no manejo de pastagem por meio da métrica de altura do pasto. O aplicativo foi desenvolvido com a tecnologia Flutter/Dart e faz estimativas de massa de forragem nas áreas cadastradas, gera relatórios e produz recomendações para auxiliar no ajuste de carga em pastagens sob pastejo rotativo ou contínuo com taxa variável.
650 $aForragem
650 $aPastagem
650 $aTaxa de Lotação
653 $aAplicativo móvel
700 1 $aFERREIRA, A. P. L.
700 1 $aGENRO, T. C. M.
700 1 $aSILVEIRA, M. C. T. da
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Embrapa Pecuária Sul (CPPSUL) |
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