Data/hora: 05/06/2024 18:23:22

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	06/04/2004	
Data da última atualização: 	27/07/2007	
Autoria: 	ALMEIDA, A. M. R.; PIUGA, F. F.; MARIN, S. R. R.; SILVEIRA, C. A. da; BINNECK, E.; RIBEIRO DO VALLE, P. Z. R.	
Título: 	Powdery mildews of soybean and weeds in Brazil: identification by the ribosomal DNA sequences.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.	
Páginas: 	p. 73-74.	
Série: 	(Embrapa Soja. Documentos, 228).	
Idioma: 	Inglês	
Notas: 	Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.	
Conteúdo: 	Powdery mildew, an obligate plant parasite, is very common on soybean and weeds that grow around soybean fields. Some species are associated with specific hosts from the same botanical species while others are able to infect a wide range of hosts. Soybean plants along with Sonchus oleraceus, Emilia sonchifolia, Taraxacum officinale and Bidens pilosa are all severely infected by powdery mildew. Mycelium was collected with a small brush and transferred to new plants from the same host and kept inside a transparent plastic cage. Colonies that developed on the new leaves were washed with distilled water and the mycelium concentrated by centrifugation was used for DNA extraction using CTAB procedure. Sequencing was performed by the chain-termination method using the ABI Big Dye Terminator Cycle sequencing kit v 2.0 on an ABI PRISM model 3100.DNA sequencer. Sequences were aligned with the Clustal V package. The data demonstrated that all soybean isolates (3) belong to the species Erysiphe diffusa (Cooke & Peck) U. Braun & S. Takamatsu, formerly Microsphaera diffusa. Isolates from sunflower, Sonchus oleraceus, and Emilia sonchifolia belong to the species Golovinomyces cichoracearum (DC.) V. P. Heluta, while isolates from Taraxacum officinale Wigg. were infected by Podosphaera fusca (Fr.) U. Braun & N. Shishkoff. The sequence of the rDNA spanning ITS1, 5.8 S gene, and ITS2 for isolates from Bidens pilosa could not be identified solely by the sequence. Two species, Neoerysiphe cumminsiana (U. Braun) U. Braun and N. galeopsidis (DC.) U. Braun shared high homology with the sequence: 100% and 97%, respectively, which suggests requirement of additional morphological analysis. So far there is no report of occurrence of Neoerysiphe spp. on Bidens pilosa excepting for an invali1d record in Cuba (Amano, K. Host range and geographical distributions of the powdery mildew fungi. Japan Scientific Society Press, Tokyo, Japan, 1986).	
Categoria do assunto: 	--	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (23689-1) - UMT - PL - -- - RF 633.34072 - W927a -  - DIS