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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
26/07/2018 |
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Data da última atualização: |
26/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. |
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Afiliação: |
ANDRE LUIZ DE ANDRADE CARVALHO, UFV; LARISSA CASTRO DE REZENDE, UFV; LUCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLAVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFV; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biological Control, v. 118, p. 16-25, 2018. |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2017.11.014 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h. MenosAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions th... Mostrar Tudo |
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Palavras-Chave: |
Bioreactor; Liquid media; Produção massal. |
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Thesagro: |
Fungo Para Controle Biológico. |
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Thesaurus Nal: |
Biological control agents; Biomass production; Bioreactors; Clonostachys rosea. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02628naa a2200313 a 4500
001 2093587
005 2018-07-26
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2017.11.014$2DOI
100 1 $aCARVALHO, A. L. de A.
245 $aOptimizing the mass production of Clonostachys rosea by liquid-state fermentation.$h[electronic resource]
260 $c2018
520 $aAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h.
650 $aBiological control agents
650 $aBiomass production
650 $aBioreactors
650 $aClonostachys rosea
650 $aFungo Para Controle Biológico
653 $aBioreactor
653 $aLiquid media
653 $aProdução massal
700 1 $aREZENDE, L. C. de
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aPINTO, Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V.
700 1 $aBETTIOL, W.
773 $tBiological Control$gv. 118, p. 16-25, 2018.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
29/08/2017 |
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Data da última atualização: |
22/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
AGUIAR, D. R. de; OLIVEIRA JUNIOR, R. C. de; BELDINI, T. P.; SILVA, R. da; TAPAJÓS, R. |
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Afiliação: |
Diego Ribeiro de Aguiar, INPA; RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; Troy Patrick Beldini, UFOPA; Rodrigo da Silva, UFOPA; Raphael Tapajós, UFOPA. |
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Título: |
Evapotranspiration and control mechanisms in managed Amazonian forest in, Pará, Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
African Journal of Agricultural Research, v. 12, n. 18, p. 1577-1586, May 2017. |
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DOI: |
10.5897/AJAR2017.12146 |
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Idioma: |
Inglês |
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Conteúdo: |
This work examines whether management causes changes in evapotranspiration (ET) surface conductance (gs), aerodynamic conductance (ga) and the decoupling factor (Ω) in managed and natural forest sites in a tropical rain forest in the Amazon. The study was conducted in the Tapajós National Forest (FNT) in managed (logged) and natural (unlogged) forests, which have micrometeorological towers for data capture. For ET estimation, the Penman-Monteith (PM) and Eddy Covariance (EC) equations were used. The models were significantly different only for unlogged (PM 134.9±15.9 mm.month-1 and EC 100.9±11.1 mm.month-1), while the means of the logged site were PM 111.1±15.7 mm.month-1 and EC 108.5±18.3 mm.month-1. Each area has different characteristics for the surface variables, ga, gs and Ω, and therefore the sites were different from each other for the study variables. However, logged ET did not differ for the PM, while EC decreased in the year after the management intervention, and was then followed by an increase. |
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Palavras-Chave: |
Condutância aerodinâmica; Fator de dissociação; Tapajós; Vapor de água. |
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Thesagro: |
Floresta Tropical. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163139/1/DIEGO-2017.pdf
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Marc: |
LEADER 01800naa a2200241 a 4500
001 2074598
005 2021-12-22
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5897/AJAR2017.12146$2DOI
100 1 $aAGUIAR, D. R. de
245 $aEvapotranspiration and control mechanisms in managed Amazonian forest in, Pará, Brazil.$h[electronic resource]
260 $c2017
520 $aThis work examines whether management causes changes in evapotranspiration (ET) surface conductance (gs), aerodynamic conductance (ga) and the decoupling factor (Ω) in managed and natural forest sites in a tropical rain forest in the Amazon. The study was conducted in the Tapajós National Forest (FNT) in managed (logged) and natural (unlogged) forests, which have micrometeorological towers for data capture. For ET estimation, the Penman-Monteith (PM) and Eddy Covariance (EC) equations were used. The models were significantly different only for unlogged (PM 134.9±15.9 mm.month-1 and EC 100.9±11.1 mm.month-1), while the means of the logged site were PM 111.1±15.7 mm.month-1 and EC 108.5±18.3 mm.month-1. Each area has different characteristics for the surface variables, ga, gs and Ω, and therefore the sites were different from each other for the study variables. However, logged ET did not differ for the PM, while EC decreased in the year after the management intervention, and was then followed by an increase.
650 $aFloresta Tropical
653 $aCondutância aerodinâmica
653 $aFator de dissociação
653 $aTapajós
653 $aVapor de água
700 1 $aOLIVEIRA JUNIOR, R. C. de
700 1 $aBELDINI, T. P.
700 1 $aSILVA, R. da
700 1 $aTAPAJÓS, R.
773 $tAfrican Journal of Agricultural Research$gv. 12, n. 18, p. 1577-1586, May 2017.
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Embrapa Amazônia Oriental (CPATU) |
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