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|
|
| Registros recuperados : 371 | |
| 242. |  | REIS, E. M.; GASSEN, F.; SILVA, C. E. Sobrevivência de Bipolaris sorokiniana em restos culturais do trigo. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 27., 1994, Itajai. Fitopatologia Brasileira, Brasília, DF, v. 19, p. 336, ago. 1994. Suplemento. E em REUNIÃO NACIONAL DE PESQUISA DE TRIGO, 17., 1994, Passo Fundo. Resumos... Passo Fundo: EMBRAPA-CNPT, 1994. P. 92. O resumo na RENAPET apresenta mais o coautor R. T. CASA. 633.11060081- R444r.| Biblioteca(s): Embrapa Trigo. |
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| 246. | | SANTOS, H. P. dos; REIS, E. M.; BAIER, A. C. Sistemas de cultivo para triticale. I. Efeitos no rendimento de grãos e nas doenças do sistema radicular do triticale, e outras culturas de verão, em plantio direto, 1987 a 1988. In: REUNIÃO BRASILEIRA DE TRITICALE, 3., 1989, Cascavel. Anais... Cascavel: OCEPAR, 1990. p. 235-244| Biblioteca(s): Embrapa Trigo. |
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| 248. | | CAVALCANTI, N. de B.; OLIVEIRA, C. A. V.; BRITO, L. T. de L.; REIS, E. M. Algumas causas da nao adocao das tecnologias de convivencia com a seca pelos pequenos produtores rurais da regiao semi-arida do Nordeste brasileiro: um estudo de caso. In: CONGRESSO BRASILEIRO DE ECONOMIA E SOCIOLOGIA RURAL, 33., 1995, Curitiba. Política agrícola e abertura de mercado: anais. Brasília, DF: SOBER, 1995. v. 2, p. 1415-1418. E em UFV-Debate, Viçosa, n. 22/23, p.3-13, jan./dez. 1996.| Biblioteca(s): Embrapa Semiárido. |
|  |
| 249. | | SEVERO, R.; REIS, E. M.; PORTO, M. D. M.; MATSUMURA, A. T. S.; PORTO, O. M. Aspectos epidemiológicos da "queda anormal de frutos jovens de citros" no RS. Fitopatologia Brasileira, Brasília, DF, v. 18, p. 316, ago. 1993. Suplemento.| Biblioteca(s): Embrapa Trigo. |
| |
| 254. | | FORCELINI, C. A.; ECCO, M.; REIS, E. M.; MORAES, M. H. D.; MENTEN, J. O. Controle de Drechslera sorokiniana e Erysiphe graminis f. sp. tritici pelo tratamento de sementes de trigo com fungicidas. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 22., 1989, Recife. Fitopatologia Brasileira, Brasília, DF, v. 14, n. 2, p. 143, jul. 1989. Resumos.| Biblioteca(s): Embrapa Trigo. |
| |
| 256. | | REIS, E. M.; CASA, R. T.; BIANCHIN, V.; DANELLI, A. L. D. Manejo integrado de doenças do trigo. In: SANTOS, H. P. dos; FONTANELI, R. S.; SPERA, S. T. (Ed.). Sistemas de produção para cereais de inverno sob plantio direto no sul do Brasil. Passo Fundo: Embrapa Trigo, 2010. Cap. 6, p. 161-205. Cap. 6, p. 161-205.| Biblioteca(s): Embrapa Trigo. |
| |
| 257. | | BARCELLOS, A. L.; GRAZZIOTIN, C.; BIASI, T. M.; REIS, E. M.; AUGUSTIN, L. Especialização fisiológica da ferrugem da folha (Puccinia coronata cda.) da aveia, no sul do Brasil em 1994 e 1995. In: REUNIÃO DA COMISSÃO SULBRASILEIRA DE PESQUISA DE AVEIA, 16., 1996, Florianópolis. Resultados experimentais. Florianópolis: UFSC: Comissão Sulbrasileira de Pesquisa de Aveia, 1996. p. 198-200| Biblioteca(s): Embrapa Trigo. |
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| 258. | | BARCELLOS, A. L.; BIASI, T. M.; GRAZZIOTIN, C.; REIS, E. M.; AUGUSTIN, L. Especialização fisiológica da ferrugem da folha (Puccinia coronata Cda.) da aveia, no sul do Brasil. In: REUNIÃO SUL-BRASILEIRA DE PESQUISA DE AVEIA, 17., 1997, Passo Fundo. Resultados experimentais... Passo Fundo: UPF; Comissão Sul-Brasileira de Pesquisa de Aveia, 1997. p. 509-512| Biblioteca(s): Embrapa Trigo. |
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| 260. | | FONTANELI, R. S.; SANTOS, H. P. dos; REIS, E. M.; AMBROSI, I. Efeitos da rotação de culturas com pastagens anuais de inverno, sob plantio direto, no rendimento de grãos de trigo. In: REUNIÃO NACIONAL DE PESQUISA DE TRIGO, 18., 1999, Passo Fundo. Anais... Passo Fundo: Embrapa Trigo, 1999. v. 2 p. 679-683| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 371 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
21/03/2019 |
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Data da última atualização: |
21/03/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. |
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Afiliação: |
MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; FAUSTO DE SOUZA SOBRINHO, CNPGL; RODRIGO DA ROCHA FRAGOSO, CPAC; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCIO ELIAS FERREIRA, Cenargen. |
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Título: |
A phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago, 2019 |
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Idioma: |
Inglês |
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Notas: |
Plant and Animal Genome XXVII Conference (PAG). |
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Conteúdo: |
Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses. MenosRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, ... Mostrar Tudo |
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Palavras-Chave: |
Brasil. |
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Thesagro: |
Capim Urochloa; Genoma. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194810/1/abstract-PAG-XXVII.pdf
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Marc: |
LEADER 02499nam a2200205 a 4500
001 2107378
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008 2019 bl uuuu u00u1 u #d
100 1 $aPESSOA FILHO, M. A. C. de P.
245 $aA phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing.$h[electronic resource]
260 $aIn: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago$c2019
500 $aPlant and Animal Genome XXVII Conference (PAG).
520 $aRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses.
650 $aCapim Urochloa
650 $aGenoma
653 $aBrasil
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aFERREIRA, M. E.
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