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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
11/01/2018 |
Data da última atualização: |
27/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
DOI: |
10.1016/j.domaniend.2017.03.003 |
Idioma: |
Inglês |
Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
Thesaurus Nal: |
DNA fragmentation; heat stress. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02716naa a2200277 a 4500 001 2085044 005 2023-01-27 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1016/j.domaniend.2017.03.003$2DOI 100 1 $aASCARI, I. J. 245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource] 260 $c2017 520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. 650 $aDNA fragmentation 650 $aheat stress 653 $aMitochondrial membrane potential 653 $aROS production 653 $aThermoprotection 700 1 $aALVES, N. G. 700 1 $aJASMIN, J. 700 1 $aLIMA, R. R. 700 1 $aQUINTAO, C. C. R. 700 1 $aOBERLENDER, G. 700 1 $aMORAES, E. A. 700 1 $aCAMARGO, L. S. de A. 773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
18/08/2009 |
Data da última atualização: |
18/08/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; CARVALHO, M. da C. S.; SILVA FILHO, J. L. da; FERREIRA, G. B. |
Afiliação: |
FLÁVIA CRISTINA DOS SANTOS, EMBRAPA MILHO E SORGO; MANOEL RICARDO DE ALBUQUERQUE FILHO, EMBRAPA MILHO E SORGO; MARIA DA CONCEIÇÃO SANTANA CARVALHO, EMBRAPA ALGODÃO; JOÃO LUÍS DA SILVA FILHO, EMBRAPA ALGODÃO; GILVAN BARBOSA FERREIRA, EMBRAPA RORAIMA. |
Título: |
Gessagem para o algodoeiro cultivado sob sistema integração lavoura-pecuária no Cerrado da Bahia. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios. Fortaleza: UFC: SBCS, 2009. |
Idioma: |
Português |
Palavras-Chave: |
Oeste baiano. |
Thesagro: |
Algodão; Gesso. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00696naa a2200193 a 4500 001 1278512 005 2009-08-18 008 2009 bl uuuu u00u1 u #d 100 1 $aSANTOS, F. C. dos 245 $aGessagem para o algodoeiro cultivado sob sistema integração lavoura-pecuária no Cerrado da Bahia. 260 $c2009 650 $aAlgodão 650 $aGesso 653 $aOeste baiano 700 1 $aALBUQUERQUE FILHO, M. R. de 700 1 $aCARVALHO, M. da C. S. 700 1 $aSILVA FILHO, J. L. da 700 1 $aFERREIRA, G. B. 773 $tIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios. Fortaleza: UFC: SBCS, 2009.
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