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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
10/03/2022 |
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Data da última atualização: |
10/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. E. F.; ARRAIS, A. M.; MELLO, M. R. B. DE; VERGANI, G. B.; FIGUEIRA, L. M.; ESTEVES, S. N.; PEREIRA, V. S. do A.; GARCIA, A. R.; BARTLEWSKI, P. M.; FONSECA, J. F. da. |
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Afiliação: |
MARIA EMILIA FRANCO OLIVEIRA, UNESP; ALINE MATOS ARRAIS, UFRRJ; MARCO ROBERTO BOURG DE MELLO, UFRRJ; GABRIEL BRUN VERGANI, UNESP; LUCAS MACHADO FIGUEIRA, UFF; SERGIO NOVITA ESTEVES, CPPSE; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; ALEXANDRE ROSSETTO GARCIA, CPPSE; PAWEL MIECZYSLAW BARTLEWSKI, University of Guelph; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, dec., 2021. |
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Páginas: |
9 p. |
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DOI: |
10.1111/rda.14077 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n= 21) or 12-day (G-12SOV, n= 21) progesterone (P4)- based estruses synchronization protocol coupled with superovula-tory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6? 7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary be-tween the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G- 12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p> .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p< .05) in G-12SOV (12.3± 1.7/12.1± 1.9) than in G-9SOV (7.9 ± 1.4/8.2± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p> .05) in G - 1 2SOV (5.8± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles >2 mm in diameter) at the sixth pFSH dose (at P4- device removal) was positively correlated (p < .05) with the number of CL (r= .95) and viable embryos (r= .91) in G-12SOV. The ewes with >10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOVgroup had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with >10 ovulations are main contributors to viable embryo production in Morada Nova ewes. MenosThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n= 21) or 12-day (G-12SOV, n= 21) progesterone (P4)- based estruses synchronization protocol coupled with superovula-tory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6? 7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary be-tween the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G- 12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p> .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p< .05) in G-12SOV (12.3± 1.7/12.1± 1.9) than in G-9SOV (7.9 ± 1.4/8.2± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p> .05) in G - 1 2SOV (5.8± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles >2 mm in diameter) at the sixth pFSH dose (at P4- device removal) was positively correlated (p < .05) with the number of CL (r= .95) and viable embryos (r= .91) in G-12SOV. The ewes with >10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOVgroup ha... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Naturalized sheep breed; Non surgical embryo recovery; NSER. |
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Thesaurus Nal: |
Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02798naa a2200313 a 4500
001 2140721
005 2022-03-10
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.14077$2DOI
100 1 $aOLIVEIRA, M. E. F.
245 $aStudy of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing.$h[electronic resource]
260 $c2021
300 $a9 p.
520 $aThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n= 21) or 12-day (G-12SOV, n= 21) progesterone (P4)- based estruses synchronization protocol coupled with superovula-tory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6? 7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary be-tween the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G- 12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p> .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p< .05) in G-12SOV (12.3± 1.7/12.1± 1.9) than in G-9SOV (7.9 ± 1.4/8.2± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p> .05) in G - 1 2SOV (5.8± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles >2 mm in diameter) at the sixth pFSH dose (at P4- device removal) was positively correlated (p < .05) with the number of CL (r= .95) and viable embryos (r= .91) in G-12SOV. The ewes with >10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOVgroup had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with >10 ovulations are main contributors to viable embryo production in Morada Nova ewes.
650 $aSuperovulation
653 $aIn vivo embryo production
653 $aNaturalized sheep breed
653 $aNon surgical embryo recovery
653 $aNSER
700 1 $aARRAIS, A. M.
700 1 $aMELLO, M. R. B. DE
700 1 $aVERGANI, G. B.
700 1 $aFIGUEIRA, L. M.
700 1 $aESTEVES, S. N.
700 1 $aPEREIRA, V. S. do A.
700 1 $aGARCIA, A. R.
700 1 $aBARTLEWSKI, P. M.
700 1 $aFONSECA, J. F. da
773 $tReproduction in Domestic Animals, dec., 2021.
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| 6. |  | PIANA, Z.; SILVA, W. R. da. Respostas de sementes de milho, com diferentes níveis de vigor, a disponibilidade hídrica. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 9, p. 1525-1531, set. 1998. Título em inglês: Responses of corn seeds with different levels of vigor to water availability.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | SILVA, W. R. da; RASEIRA, M. do C. B. Brilho e reversão de cor em novas seleções de amora-preta. In:SIMPÓSIO NACIONAL DO MORANGO, 6.; ENCONTRO SOBRE PEQUENAS FRUTAS E FRUTAS NATIVAS DO MERCOSUL, 5., 2012, Pelotas. Palestras e resumos... Pelotas: Embrapa Clima Temperado, 2012. 231 p. Organizadores: Marcia Vizzotto, Carlos Reisser Júnior, Rosa Lia Barbieri, Rodrigo Cezar Franzon.| Tipo: Resumo em Anais de Congresso |
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