Data/hora: 18/06/2024 03:20:01

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais.
Data corrente: | 17/05/2012
Data da última atualização: | 15/08/2017
Autoria: | SILVA, R. de C; LUIS, Z. G.; PEREIRA, J. E. S.
Afiliação: | RAFAEL DE CARVALHO SILVA, UNIVERSIDADE FEDERAL DO AMAZONAS; ZANDERLUCE GOMES LUIS, UNB; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN.
Título: | Short-term storage in vitro and large-scale propagation of grapevine genotypes.
Ano de publicação: | 2012
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 3, p. 344-350, mar. 2012.
Idioma: | Inglês
Notas: | Título em português: Conservação de curto prazo in vitro e propagação em larga escala de genótipos de videira.
Conteúdo: | The objective of this work was to evaluate the large?scale propagation of grapevine genotypes after short?term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short?term storage, the shoots were propagated in up to five successive subcultures, to assess the large?scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short?term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 ?mol L?1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20oC, can be micropropagated in large scale.
Palavras-Chave: | Conservação in vitro; Crescimento mínimo; In vitro conservation; Minimal growth.
Thesagro: | Germoplasma; Micropropagação.
Thesaurus Nal: | ex situ conservation; germplasm; micropropagation; Vitis.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/62932/1/Short-term.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)

Exemplares:

CENARGEN - (34038-1) - UPC - AP - PP - 630.5 -  -  - DIS
AI-SEDE - (53273-1) - UPE - AP - PP - 630.72081 - p474 -  - DIS