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| Registros recuperados : 4 | |
| 1. |  | JOSLIN, A.; MARKEWITZ, D.; MORRIS, L.; KATO, O. R.; FIGUEIREDO, R.; OLIVEIRA, F. de A. Crescimento de cinco espécies nativas em successão natural na Amazônia Oriental. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | JOSLIN, A. H.; MARKEWITZ, D.; MORRIS, L. A.; OLIVEIRA, F. de A.; FIGUEIREDO, R. de O.; KATO, O. R. Five native tree species and manioc under slash-and-mulch agroforestry in the eastern Amazon of Brazil: plant growth and soil responses. Agroforestry Systems, v. 81, n. 1, p. 1-14, 2011.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | JOSLIN, A. H.; MARKEWITZ, D.; MORRIS, L. A.; OLIVEIRA, F. de A.; FIGUEIREDO, R. O.; KATO, O. R. Soil and plant N-budget 1 year after planting of a slash-and-mulch agroforestry system in the eastern Amazon of Brazil. Agroforestry Systems, v. 87, n. 6, p. 1339-1349, Dec. 2013.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 4 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
07/10/2022 |
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Data da última atualização: |
07/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MACEDO, T. J. S.; MENEZES, V. G.; BARBERINO, R. S.; SILVA, R. L. S.; GOUVEIA, B. B.; MONTE, A. P. O.; LINS, T. L. B. G.; SANTOS, J. M. S.; BEZERRA, M. E. S.; WISCHRAL, A.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; BATISTAM A. M.; MATOS, M. H. T. |
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Afiliação: |
T. J. S. MACEDO, UNIVASF; V. G. MENEZES, UNIVASF; R. S. BARBERINO, UNIVASF; R. L. S. SILVA, UNIVASF; B. B. GOUVEIA, UNIVASF; A. P. O. MONTE, UNIVASF; T. L. B. G. LINS, UNIVASF; J. M. S. SANTOS, UNIVASF; M. E. S. BEZERRA, UNIVASF; A. WISCHRAL, UFRPE; M. A. A. QUEIROZ, UNIVASF; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; A. M. BATISTA, URFPE; M. H. T. MATOS., UNIVASF. |
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Título: |
Leptin decreases apoptosis and promotes the activation of primordial follicles through the phosphatidylinositol-3-kinase/protein kinase B pathway in cultured ovine ovarian tissue. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Zygote, v. 29, n. 6, p. 445-451, 2021. |
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DOI: |
https://doi.org/10.1017/S0967199421000034 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effects of leptin on primordial follicle survival and activation after in vitro culture of ovine ovarian tissue and if leptin acts through the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) pathway. Ovarian fragments were fixed for histology (fresh control) or cultured for 7 days in control medium (α-MEMþ) alone or supplemented with leptin (1, 5, 10, 25 or 50 ng/ml). Follicle morphology, activation and apoptosis were analyzed. Next, the fragments were cultured in the medium that showed the best results in the absence or the presence of the PI3K inhibitor (LY294002), and immunohistostaining of p-Akt protein was assessed. After culture, the percentage of normal follicles decreased (P < 0.05) in all treatments compared with the fresh control. Moreover, control medium and 1 ng/ml leptin had similar (P > 0.05) percentages of normal follicles, which were significantly higher than those in other treatments. However, culture with 1 ng/ml leptin maintained apoptosis similarly (P > 0.05) to that of the fresh control and lower (P < 0.05) than that in α-MEMþ. Leptin did not influence follicle activation (P > 0.05) compared with the control medium (α-MEMþ). Culture in 1 ng/ml leptin with LY294002 decreased the normal follicles and increased apoptosis, inhibited follicle activation (P < 0.05), and reduced p-Akt immunostaining, compared with the medium containing 1 ng/ml leptin without PI3K inhibitor. In conclusion, leptin at 1 ng/ml reduces apoptosis and promotes the activation of primordial follicles compared with the fresh control after in vitro culture of ovine ovarian tissue possibly through the PI3K/Akt pathway. MenosThis study evaluated the effects of leptin on primordial follicle survival and activation after in vitro culture of ovine ovarian tissue and if leptin acts through the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) pathway. Ovarian fragments were fixed for histology (fresh control) or cultured for 7 days in control medium (α-MEMþ) alone or supplemented with leptin (1, 5, 10, 25 or 50 ng/ml). Follicle morphology, activation and apoptosis were analyzed. Next, the fragments were cultured in the medium that showed the best results in the absence or the presence of the PI3K inhibitor (LY294002), and immunohistostaining of p-Akt protein was assessed. After culture, the percentage of normal follicles decreased (P < 0.05) in all treatments compared with the fresh control. Moreover, control medium and 1 ng/ml leptin had similar (P > 0.05) percentages of normal follicles, which were significantly higher than those in other treatments. However, culture with 1 ng/ml leptin maintained apoptosis similarly (P > 0.05) to that of the fresh control and lower (P < 0.05) than that in α-MEMþ. Leptin did not influence follicle activation (P > 0.05) compared with the control medium (α-MEMþ). Culture in 1 ng/ml leptin with LY294002 decreased the normal follicles and increased apoptosis, inhibited follicle activation (P < 0.05), and reduced p-Akt immunostaining, compared with the medium containing 1 ng/ml leptin without PI3K inhibitor. In conclusion, leptin at 1 ng/ml reduc... Mostrar Tudo |
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Palavras-Chave: |
Adipocina; Danos no DNA; Folículos ovarianos; Leptina; Oócito; Primordial folículo. |
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Thesagro: |
Ovelha. |
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Thesaurus NAL: |
Adipokines; DNA damage; Leptin; Oocytes; Ovarian follicles; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02982naa a2200445 a 4500
001 2147186
005 2022-10-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199421000034$2DOI
100 1 $aMACEDO, T. J. S.
245 $aLeptin decreases apoptosis and promotes the activation of primordial follicles through the phosphatidylinositol-3-kinase/protein kinase B pathway in cultured ovine ovarian tissue.
260 $c2021
520 $aThis study evaluated the effects of leptin on primordial follicle survival and activation after in vitro culture of ovine ovarian tissue and if leptin acts through the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) pathway. Ovarian fragments were fixed for histology (fresh control) or cultured for 7 days in control medium (α-MEMþ) alone or supplemented with leptin (1, 5, 10, 25 or 50 ng/ml). Follicle morphology, activation and apoptosis were analyzed. Next, the fragments were cultured in the medium that showed the best results in the absence or the presence of the PI3K inhibitor (LY294002), and immunohistostaining of p-Akt protein was assessed. After culture, the percentage of normal follicles decreased (P < 0.05) in all treatments compared with the fresh control. Moreover, control medium and 1 ng/ml leptin had similar (P > 0.05) percentages of normal follicles, which were significantly higher than those in other treatments. However, culture with 1 ng/ml leptin maintained apoptosis similarly (P > 0.05) to that of the fresh control and lower (P < 0.05) than that in α-MEMþ. Leptin did not influence follicle activation (P > 0.05) compared with the control medium (α-MEMþ). Culture in 1 ng/ml leptin with LY294002 decreased the normal follicles and increased apoptosis, inhibited follicle activation (P < 0.05), and reduced p-Akt immunostaining, compared with the medium containing 1 ng/ml leptin without PI3K inhibitor. In conclusion, leptin at 1 ng/ml reduces apoptosis and promotes the activation of primordial follicles compared with the fresh control after in vitro culture of ovine ovarian tissue possibly through the PI3K/Akt pathway.
650 $aAdipokines
650 $aDNA damage
650 $aLeptin
650 $aOocytes
650 $aOvarian follicles
650 $aSheep
650 $aOvelha
653 $aAdipocina
653 $aDanos no DNA
653 $aFolículos ovarianos
653 $aLeptina
653 $aOócito
653 $aPrimordial folículo
700 1 $aMENEZES, V. G.
700 1 $aBARBERINO, R. S.
700 1 $aSILVA, R. L. S.
700 1 $aGOUVEIA, B. B.
700 1 $aMONTE, A. P. O.
700 1 $aLINS, T. L. B. G.
700 1 $aSANTOS, J. M. S.
700 1 $aBEZERRA, M. E. S.
700 1 $aWISCHRAL, A.
700 1 $aQUEIROZ, M. A. A.
700 1 $aARAUJO, G. G. L. de
700 1 $aBATISTAM A. M.
700 1 $aMATOS, M. H. T.
773 $tZygote$gv. 29, n. 6, p. 445-451, 2021.
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